GST transcriptional changes induced by a toxic Microcystis aeruginosa strain in two bivalve species during exposure and recovery phases

Paulo, Antas; Carneiro, Mariana; Reis, Bruno; Castelo-Branco, Raquel; Azevedo, Joana; Urbatzka, Ralph; Campos, Alexandre; Vasconcelos, Vı́tor; Martins, José

doi:10.1007/s10646-018-1980-y

Cited by 8 publications

(4 citation statements)

References 38 publications

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“…Working with bivalves of several aquatic habitats, Carneiro et al [38] showed that these mollusks presented specific adaptive biotransformation responses to MCs and other cyanobacteria compounds supported by the modulation of distinct cGST classes. Overall, results support the relative importance of the GST system as a protective mechanism to cope with MC-producing cyanobacterial blooms exposure which seems to be different between the bivalve species [56]. Despite the ability of cyanotoxins uptake and elimination, physiological alterations along with biochemical and genotoxic effects have been shown to be induced in bivalves by toxic cyanobacteria [39,57].…”

Section: In Vivo Studiessupporting

confidence: 54%

Review on Cyanobacterial Studies in Portugal: Current Impacts and Research Needs

et al. 2021

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Cyanobacteria have long been associated with harmful effects on humans, animals and aquatic biota. Cyanotoxins are their most toxic metabolite. This review summarizes the current research, impacts and future needs in cyanobacterial studies undertaken in Portugal, the southernmost country of Europe, and with a recent multiplication of cyanotoxicity due to climate change events. Microcystins are still the most prevalent, studied and the only regulated cyanotoxins in Portuguese freshwater systems much like most European countries. With the development of some tools, particularly in molecular studies, the recent discovery of cylindrospermopsins, anatoxins and saxitoxins, both genes and toxins, in North and Center ecosystems of our country highlight current impacts that overall communities are facing with increased risks of exposure and uptake to cyanotoxins. Research needs encompass the expansion of studies at all aspects due to the uprising of these cyanotoxins and reinforces the urgent need of increasing the frequency of surveillance to achieve tangible effects of cyanotoxins in Portugal to ultimately implement regulations on cylindrospermopsins, anatoxins and saxitoxins worldwide.

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Section: In Vivo Studiessupporting

confidence: 54%

Review on Cyanobacterial Studies in Portugal: Current Impacts and Research Needs

et al. 2021

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“…No other mechanisms have been fully elucidated to date, but a few have been hypothesized. GSTs have been implicated in the detoxification of MCs in some higher plants, invertebrates and vertebrates by first conjugating to the MCs to be targeted for removal by further processes (Pflugmacher et al, 1998;Campos and Vasconcelos, 2010;Antas et al, 2018). While the role of GSTs in bacterial degradation of MCs has not been confirmed, they can play a role in biodegradation of other toxic compounds (Allocati et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Insight Into the Molecular Mechanisms for Microcystin Biodegradation in Lake Erie and Lake Taihu

et al. 2019

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“…Induction of sigma 1-class GST transcripts was observed in Ruditapes philippinarum upon 24-h exposure to toxic Microcystis extract. The post-exposure phase was characterized by an early induction (24 h) of sigma 1 and mu transcripts and a later induction (72 h) of the four analyzed GST transcripts (sigma 1, sigma 2, pi, and mu-class GST transcripts) [52]. Also concerning enzymatic activity and protein expression, significant alterations in GST according to bivalve species, were reported.…”

Section: Metabolic Responses Of Mussels To Toxic Cyanobacteriamentioning

confidence: 96%

Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

Oliveira

Díez-Quijada

Turkina

et al. 2020

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Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species are affected by the exposure to freshwater cyanobacteria and cyanotoxins. So far, most of the knowledge has been gathered from freshwater bivalve molluscs. This work aimed to infer the sensitivity of the marine mussel Mytilus galloprovincialis to single as well as mixed toxic cyanobacterial cultures and the underlying molecular responses mediated by toxic cyanobacteria. For this purpose, a mussel exposure experiment was outlined with two toxic cyanobacteria species, Microcystis aeruginosa and Chrysosporum ovalisporum at 1 × 105 cells/mL, resembling a natural cyanobacteria bloom. The estimated amount of toxins produced by M. aeruginosa and C. ovalisporum were respectively 0.023 pg/cell of microcystin-LR (MC-LR) and 7.854 pg/cell of cylindrospermopsin (CYN). After 15 days of exposure to single and mixed cyanobacteria, a depuration phase followed, during which mussels were fed only non-toxic microalga Parachlorella kessleri. The results showed that the marine mussel is able to filter toxic cyanobacteria at a rate equal or higher than the non-toxic microalga P. kessleri. Filtration rates observed after 15 days of feeding toxic microalgae were 1773.04 mL/ind.h (for M. aeruginosa), 2151.83 mL/ind.h (for C. ovalisporum), 1673.29 mL/ind.h (for the mixture of the 2 cyanobacteria) and 2539.25 mL/ind.h (for the non-toxic P. kessleri). Filtering toxic microalgae in combination resulted in the accumulation of 14.17 ng/g dw MC-LR and 92.08 ng/g dw CYN. Other physiological and biochemical endpoints (dry weight, byssus production, total protein and glycogen) measured in this work did not change significantly in the groups exposed to toxic cyanobacteria with regard to control group, suggesting that mussels were not affected with the toxic microalgae. Nevertheless, proteomics revealed changes in metabolism of mussels related to diet, specially evident in those fed on combined cyanobacteria. Changes in metabolic pathways related with protein folding and stabilization, cytoskeleton structure, and gene transcription/translation were observed after exposure and feeding toxic cyanobacteria. These changes occur in vital metabolic processes and may contribute to protect mussels from toxic effects of the toxins MC-LR and CYN.

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GST transcriptional changes induced by a toxic Microcystis aeruginosa strain in two bivalve species during exposure and recovery phases

Cited by 8 publications

References 38 publications

Review on Cyanobacterial Studies in Portugal: Current Impacts and Research Needs

Review on Cyanobacterial Studies in Portugal: Current Impacts and Research Needs

Insight Into the Molecular Mechanisms for Microcystin Biodegradation in Lake Erie and Lake Taihu

Physiological and Metabolic Responses of Marine Mussels Exposed to Toxic Cyanobacteria Microcystis aeruginosa and Chrysosporum ovalisporum

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