GST polymorphisms and early-onset coronary artery disease in young South African Indians

Phulukdaree, Alisa; Khan, Sajidah; Moodley, Devapregasan; Chuturgoon, Anil A.

doi:10.7196/samj.5520

Cited by 27 publications

(17 citation statements)

References 20 publications

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“…Phulukdaree and co-workers reported biochemical measures of CAD patients and healthy controls in 2012 12. As expected, in our study, CAD patients presented with more conventional risk factors, such as higher body mass index (BMI), higher total and LDL cholesterol and triglyceride levels, and a higher prevalence of type 2 diabetes mellitus than the control groups.…”

Section: Sirt1 Rs7895833supporting

confidence: 83%

Sirtuin 1 rs1467568 and rs7895833 in South African Indians with early-onset coronary artery disease

Ramkaran¹,

Phulukdaree²,

Khan³

et al. 2016

CVJA

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SummaryBackgroundSirtuin 1 (SIRT1), a class III histone deacetylase, has been identified as a candidate molecule affecting the epigenetic mechanisms of cardiovascular disease (CVD). Previous studies have shown that some SIRT1 single-nucleotide polymorphisms (SNPs) are associated with body mass index, diabetes, blood pressure, cholesterol metabolism and coronary artery calcification. We investigated two A>G SIRT1 SNPs, rs1467568 and rs7895833, in young South African (SA) Indians with coronary artery disease (CAD) and compared them to Indian and black controls.MethodsFor rs1467568, a total of 287 subjects were recruited into this study (104 CAD patients, 99 age-, gender- and race-matched controls, and 84 age- and gender-matched black controls). For rs7895833, a total of 281 subjects were recruited into this study (100 CAD patients, 99 age-, gender- and race-matched controls, and 82 age- and gender-matched black controls). All patients were male, of Indian ethnicity, stable CAD confirmed on angiography, mean age 37.5 years; range 24–45. All subjects were genotyped using TaqMan SNP genotyping assays.ResultsThe variant allele for both SNPs was found at a higher frequency in the total Indian group compared to the total black population (rs1467568: 41 vs 18.5%, respectively, p < 0.0001, OR = 3.190, 95% CI: 2.058–40943; and rs7895833: 41 vs 22%, respectively, p < 0.0001, OR = 2.466, 95% CI: 1.620– 3.755). Indian controls presented with a higher frequency for both SNPs compared to black controls (rs1467568: 40 vs 18.5%, respectively, p < 0.0001, OR = 2.996, 95% CI: 1.850– 4.853; and rs7895833: 41 vs 22%, respectively, p < 0.0001, OR = 2.513, 95% CI: 1.578–4.004). No difference was seen in the distribution of both SNPs between CAD patients and either control group. We did not observe any association between the SNPs and clinical parameters in CAD patients and controls.Conclusion:Both SNP variant alleles occurred more frequently in SA Indians than in SA blacks. A larger study group and further analysis is required to assess whether these SIRT1 SNPs may serve as risk factors that contribute to Indians developing early-onset CAD.

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Section: Sirt1 Rs7895833supporting

confidence: 83%

Sirtuin 1 rs1467568 and rs7895833 in South African Indians with early-onset coronary artery disease

Ramkaran¹,

Phulukdaree²,

Khan³

et al. 2016

CVJA

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“…Wang et al 38 also observed a significant association between the GSTM1 -null polymorphism and CHD risk in the Chinese population. Phulukdaree et al 39 documented that a 2.6-fold higher risk for developing CAD in young South African Indians was attributed to the GSTM1 null genotype. Our results are not in concordance with the results of Girisha et al 32 and Singh et al ,17 who observed no significant association of GSTM1 null genotype with CAD and acute myocardial infarction in a north Indian population, respectively.…”

Section: Discussionmentioning

confidence: 99%

Association of GSTT1 and GSTM1 gene polymorphisms with coronary artery disease in North Indian Punjabi population: a case-control study

Bhat

Gandhi

2016

Postgraduate Medical Journal

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“…Since, helminth parasites lack p-450 monoxygenases, the Phase-II biotransformations are carried out by GST isoforms and hence are of cardinal importance [ 27 ]. GSTs perform several important functions in the body and are associated with several pathological conditions including cancer [ 28 ], rheumatoid arthritis [ 29 ], osteoporosis [ 30 ], renal dysfunctions [ 31 ], cardiovascular diseases [ 32 , 33 ], and Alzheimer’s disease [ 34 ]. GSTs have been purified and characterized from various helminth parasites including nematodes, trematodes, and cestodes such as Clonorchis sinensis [ 35 ], Setaria digitata [ 36 ], Schistosoma mansoni [ 37 ], Schistosoma japonicum [ 38 ], Fasciola hepatica [ 39 ], Necator americanus [ 40 ], and Echinococcus granulosus [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Partial purification and characterization of glutathione S-transferase from the somatic tissue of Gastrothylax crumenifer (Trematoda: Digenea)

et al. 2017

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Aim:Aim of the present study was to carry out the partial purification and biochemical characterization of glutathione S-transferase (GST) from the somatic tissue of ruminal amphistome parasite, Gastrothylax crumenifer (Gc) infecting Indian water buffalo (Bubalus bubalis).Materials and Methods:The crude somatic homogenate of Gc was subjected to progressive ammonium sulfate precipitation followed by size exclusion chromatography in a Sephacryl S 100-HR column. The partially purified GST was assayed spectrophotometrically, and the corresponding enzyme activity was also recorded in polyacrylamide gel. GST isolated from the amphistome parasite was also exposed to variable changes in temperature and the pH gradient of the assay mixture.Results:The precipitated amphistome GST molecules showed maximum activity in the sixth elution fraction. The GST subunit appeared as a single band in the reducing polyacrylamide gel electrophoresis with an apparent molecular weight of 26 kDa. The GST proteins were found to be fairly stable up to 37°C, beyond this the activity got heavily impaired. Further, the GST obtained showed a pH optima of 7.5.Conclusion:Present findings showed that GST from Gc could be conveniently purified using gel filtration chromatography. The purified enzyme showed maximum stability and activity at 4°C.

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GST polymorphisms and early-onset coronary artery disease in young South African Indians

Cited by 27 publications

References 20 publications

Sirtuin 1 rs1467568 and rs7895833 in South African Indians with early-onset coronary artery disease

Sirtuin 1 rs1467568 and rs7895833 in South African Indians with early-onset coronary artery disease

Association of GSTT1 and GSTM1 gene polymorphisms with coronary artery disease in North Indian Punjabi population: a case-control study

Partial purification and characterization of glutathione S-transferase from the somatic tissue of Gastrothylax crumenifer (Trematoda: Digenea)

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