GsSNAP33 , a novel Glycine soja SNAP25-type protein gene: Improvement of plant salt and drought tolerances in transgenic Arabidopsis thaliana

Nisa, Zaibun; Mallano, Ali Inayat; Yu, Youjian; Chen, Chao; Duan, Xiangbo; Amanullah, Sikandar; Kousar, Abida; Baloch, Abdul Wahid; Sun, Xiaofen; Tabys, Dina; Zhu, Yanming

doi:10.1016/j.plaphy.2017.07.029

Cited by 17 publications

(14 citation statements)

References 53 publications

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“…Meanwhile, vesicle trafficking may also be influenced by the ion gradients they establish within the expanding cell [ 43 ]. Several reports have shown that over-expression of plant SNARE genes could improve the salt and drought tolerance of transgenic plants [ 44 , 45 , 46 ], which indicates that SNARE proteins might be involved in membrane stability, the K + /Na + ratio, and antioxidant machinery [ 45 ]. NCBI SmartBlast analysis revealed that the IpSNARE (IpSR14) protein has a high sequence identity (47% amino acid identity under 77% query cover) to a SNARE-like superfamily protein from A. thaliana (NP_567842.1; At4g30240).…”

Section: Discussionmentioning

confidence: 99%

Functional Identification of Salt-Stress-Related Genes Using the FOX Hunting System from Ipomoea pes-caprae

Zhang

Zheng

et al. 2018

IJMS

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Ipomoea pes-caprae is a seashore halophytic plant and is therefore a good model for studying the molecular mechanisms underlying salt and stress tolerance in plant research. Here, we performed Full-length cDNA Over-eXpressor (FOX) gene hunting with a functional screening of a cDNA library using a salt-sensitive yeast mutant strain to isolate the salt-stress-related genes of I. pes-caprae (IpSR genes). The library was screened for genes that complemented the salt defect of yeast mutant AXT3 and could grow in the presence of 75 mM NaCl. We obtained 38 candidate salt-stress-related full-length cDNA clones from the I. pes-caprae cDNA library. The genes are predicted to encode proteins involved in water deficit, reactive oxygen species (ROS) scavenging, cellular vesicle trafficking, metabolic enzymes, and signal transduction factors. When combined with the quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analyses, several potential functional salt-tolerance-related genes were emphasized. This approach provides a rapid assay system for the large-scale screening of I. pes-caprae genes involved in the salt stress response and supports the identification of genes responsible for the molecular mechanisms of salt tolerance.

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Section: Discussionmentioning

confidence: 99%

Functional Identification of Salt-Stress-Related Genes Using the FOX Hunting System from Ipomoea pes-caprae

Zhang

Zheng

et al. 2018

IJMS

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“…Three Qbc-SNAREs have been identified in the genomes of Solanum lycopersicum and its relative wild species ( S. chilense , S. pimpinellifolium , S. habrochaites , and S. pennellii ), namely SlSNAP33.1 , SlSNAP33.2 , and SlSNAP30 [ 18 ]. The transcripts accumulation of the three AtSNAP33 orthologs SlSNAP33.2, SchSNAP33.2 , and GsSNAP33 from Glycine soja increase during salt stress [ 18 , 19 ], suggest that SNAP33 orthologues play a role in the plant adaptation to abiotic stress.…”

Section: Introductionmentioning

confidence: 99%

The Exocytosis Associated SNAP25-Type Protein, SlSNAP33, Increases Salt Stress Tolerance by Modulating Endocytosis in Tomato

Salinas-Cornejo

Madrid-Espinoza

Verdugo

et al. 2021

Plants

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In plants, vesicular trafficking is crucial for the response and survival to environmental challenges. The active trafficking of vesicles is essential to maintain cell homeostasis during salt stress. Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) are regulatory proteins of vesicular trafficking. They mediate membrane fusion and guarantee cargo delivery to the correct cellular compartments. SNAREs from the Qbc subfamily are the best-characterized plasma membrane SNAREs, where they control exocytosis during cell division and defense response. The Solanum lycopersicum gene SlSNAP33.2 encodes a Qbc-SNARE protein and is induced under salt stress conditions. SlSNAP33.2 localizes on the plasma membrane of root cells of Arabidopsis thaliana. In order to study its role in endocytosis and salt stress response, we overexpressed the SlSNAP33.2 cDNA in a tomato cultivar. Constitutive overexpression promoted endocytosis along with the accumulation of sodium (Na+) in the vacuoles. It also protected the plant from cell damage by decreasing the accumulation of hydrogen peroxide (H2O2) in the cytoplasm of stressed root cells. Subsequently, the higher level of SlSNAP33.2 conferred tolerance to salt stress in tomato plants. The analysis of physiological and biochemical parameters such as relative water content, the efficiency of the photosystem II, performance index, chlorophyll, and MDA contents showed that tomato plants overexpressing SlSNAP33.2 displayed a better performance under salt stress than wild type plants. These results reveal a role for SlSNAP33.2 in the endocytosis pathway involved in plant response to salt stress. This research shows that SlSNAP33.2 can be an effective tool for the genetic improvement of crop plants.

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“…With the changing of global climate, drought stress is becoming a major environmental problem affecting crop growth, development, and production ( Ahuja et al, 2010 ). GsSNAP33, the G. soja homolog of GhSNAP33, is involved in plant tolerance to drought and salt stress in Arabidopsis ( Nisa et al, 2017 ). We found that GhSNAP33 overexpression was enhanced by ABA and PEG6000 treatments and ectopic expression of GhSNAP33 increased yeast tolerance to mannitol.…”

Section: Discussionmentioning

confidence: 99%

“…HvSNAP34, a SNAP25-type protein in barley, associates with syntaxin (ROR2) and contributes to powdery mildew resistance ( Collins et al, 2003 ). Cynanchum komarovii CkSNAP33 enhances Arabidopsis disease resistance to Verticillium dahliae ( Wang et al, 2017 ), and Glycine soja GsSNAP33 increases tolerance to salt and drought stress in transgenic Arabidopsis ( Nisa et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

GhSNAP33, a t-SNARE Protein From Gossypium hirsutum, Mediates Resistance to Verticillium dahliae Infection and Tolerance to Drought Stress

et al. 2018

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Soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) proteins mediate membrane fusion and deliver cargo to specific cellular locations through vesicle trafficking. Synaptosome-associated protein of 25 kDa (SNAP25) is a target membrane SNARE that drives exocytosis by fusing plasma and vesicular membranes. In this study, we isolated GhSNAP33, a gene from cotton (Gossypium hirsutum), encoding a SNAP25-type protein containing glutamine (Q)b- and Qc-SNARE motifs connected by a linker. GhSNAP33 expression was induced by H2O2, salicylic acid, abscisic acid, and polyethylene glycol 6000 treatment and Verticillium dahliae inoculation. Ectopic expression of GhSNAP33 enhanced the tolerance of yeast cells to oxidative and osmotic stresses. Virus-induced gene silencing of GhSNAP33 induced spontaneous cell death and reactive oxygen species accumulation in true leaves at a later stage of cotton development. GhSNAP33-deficient cotton was susceptible to V. dahliae infection, which resulted in severe wilt on leaves, an elevated disease index, enhanced vascular browning and thylose accumulation. Conversely, Arabidopsis plants overexpressing GhSNAP33 showed significant resistance to V. dahliae, with reduced disease index and fungal biomass and elevated expression of PR1 and PR5. Leaves from GhSNAP33-transgenic plants showed increased callose deposition and reduced mycelia growth. Moreover, GhSNAP33 overexpression enhanced drought tolerance in Arabidopsis, accompanied with reduced water loss rate and enhanced expression of DERB2A and RD29A during dehydration. Thus, GhSNAP33 positively mediates plant defense against stress conditions and V. dahliae infection, rendering it a candidate for the generation of stress-resistant engineered cotton.

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GsSNAP33 , a novel Glycine soja SNAP25-type protein gene: Improvement of plant salt and drought tolerances in transgenic Arabidopsis thaliana

Cited by 17 publications

References 53 publications

Functional Identification of Salt-Stress-Related Genes Using the FOX Hunting System from Ipomoea pes-caprae

Functional Identification of Salt-Stress-Related Genes Using the FOX Hunting System from Ipomoea pes-caprae

The Exocytosis Associated SNAP25-Type Protein, SlSNAP33, Increases Salt Stress Tolerance by Modulating Endocytosis in Tomato

GhSNAP33, a t-SNARE Protein From Gossypium hirsutum, Mediates Resistance to Verticillium dahliae Infection and Tolerance to Drought Stress

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