GSNOR deficiency enhances betulin production in Betula platyphylla

Fan, Guorong; Tang, Nujiang; Huang, Yating; Yu, Zhan

doi:10.1007/s00468-018-1677-0

Cited by 9 publications

(6 citation statements)

References 36 publications

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“…Our precious study verified that SNO enhanced betulin production using BpGSNOR transgenic B. platyphylla seedlings by RNAi silencing and GSNOR inhibitor N6022, and it indicated that protein S-nitrosylation mediated betulin production in B. platyphylla, which is a pioneer hardwood tree species with rich medical triterpenoid [12,13]. Some bZIP TFs can promote secondary metabolite synthesis, such as VvbZIP36, PybZIP, and PgbZIP promotes anthocyanin accumulation in grapevine, Pear, and pomegranate, respectively [11,14,15].…”

Section: Introductionmentioning

confidence: 53%

Characteristic analysis of BpbZIP family genes and BpbZIP26 significantly enhanced triterpenoid production in Betula platyphylla under S-nitrosothiol treatment

Wang

Gao

Yang

et al. 2022

Chem. Biol. Technol. Agric.

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Background Basic leucine zipper (bZIP) transcription factors are crucial in plant development, and response to environmental stress, etc. With the development of sequencing technology and bioinformatics analysis, the bZIP family genes has been screened and identified in many plant species, but bZIP family genes has not been systematically characterized and identified their function in Betula platyphylla. Methods B. platyphylla reference genome was used to characterize bZIP family genes. The physicochemical properties, chromosome distribution, gene structure, and syntenic relationships were analyzed by bioinformatics methods. The effect of BpbZIP26 on triterpenoid production was investigated using Agrobacterium-mediated transient transformation under N6022 treatment. Results 51 bZIP family genes were identified in B. platyphylla, and named BpbZIP1–BpbZIP51 sequentially according to their positions on chromosomes. All BpbZIP genes were unevenly distributed on 14 chromosomes, and divided into 13 subgroups according to the classification of Arabidopsis thaliana bZIP proteins. 12 duplication events were detected in the B. platyphylla genome, and 28 orthologs existed between B. platyphylla and A. thaliana, 83 orthologs existed between B. platyphylla and Glycine max, and 73 orthologs existed between B. platyphylla and Populus trichocarpa. N6022 treatment changed gene expression levels of most BpbZIPs in seedlings of B. platyphylla. Among of them, N6022 treatment significantly enhanced gene expression levels of BpbZIP26 in leaves, stems and roots of B. platyphylla. BpbZIP26 mediated triterpenoid production, and N6022 treatment further enhanced triterpenoid production in BpbZIP26 overexpression calli of B. platyphylla using Agrobacterium-mediated transient transformation. Conclusion This work highlights potential BpbZIP family genes responding to S-nitrosothiol and provides candidate genes for triterpenoid production in B. platyphylla. Graphical Abstract

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Section: Introductionmentioning

confidence: 53%

Characteristic analysis of BpbZIP family genes and BpbZIP26 significantly enhanced triterpenoid production in Betula platyphylla under S-nitrosothiol treatment

Wang

Gao

Yang

et al. 2022

Chem. Biol. Technol. Agric.

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“…Our precious study veri ed that SNO enhanced betulin production using BpGSNOR transgenic B. platyphylla seedlings by RNAi silencing and GSNOR inhibitor N6022, and it indicated that protein Snitrosylation mediated betulin production in B. platyphylla, which is a pioneer hardwood tree species with rich medical triterpenoid [12,13]. Some bZIP TFs can promote secondary metabolite synthesis, such as VvbZIP36, PybZIP, and PgbZIP promotes anthocyanin accumulation in grapevine, Pear, and pomegranate, respectively [14][15][16].…”

Section: Introductionmentioning

confidence: 85%

“…Our previous study showed that N6022 treatment signi cantly enhanced triterpenoid content [12,13], and it also signi cantly increased gene expression of BpbZIP26. To investigate the function of BpbZIP26 in triterpenoid synthesis, overexpression and silencing vector of BpbZIP26 were constructed to transfer into B. platyphylla calli.…”

Section: Overexpression Of Bpbzip26 Enhanced Triterpenoid Production ...mentioning

confidence: 93%

Characteristic analysis of BpbZIP family genes and BpbZIP26 significantly enhanced triterpenoid production in Betula platyphylla under S-nitrosothiol treatment

Wang

Yang

Gao

et al. 2022

Preprint

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Background: Basic leucine zipper (bZIP) transcription factors are crucial in plant development, and response to environmental stress, etc. With the development of sequencing technology and bioinformatics analysis, the bZIP family genes has been screened and identified in many plant species, but bZIP family genes has not been systematically characterized and identified their function in B. platyphylla.Methods: B. platyphylla reference genome was used to characterize bZIP family genes. The physicochemical properties, chromosome distribution, gene structure, and syntenic relationships were analyzed by bioinformatics methods. The effect of BpbZIP26 on triterpenoid production was investigated using Agrobacterium-mediated transient transformation under N6022 treatment.Results: 51 bZIP family genes were identified in B. platyphylla, and named BpbZIP1~BpbZIP51 sequentially according to their positions on chromosomes. All BpbZIP genes were unevenly distributed on 14 chromosomes, and divided into 13 subgroups according to the classification of A. thaliana bZIP proteins. 12 duplication events were detected in the B. platyphylla genome, and 28 orthologs existed between B. platyphylla and A. thaliana, 83 orthologs existed between B. platyphylla and G. max, and 73 orthologs existed between B. platyphylla and P. trichocarpa. N6022 treatment changed gene expression levels of most BpbZIPs in seedlings of Betula platyphylla. Among of them, N6022 treatment significantly enhanced gene expression levels BpbZIP26 in leaves, stems and roots of B. platyphylla. BpbZIP26 mediated triterpenoid production, and N6022 treatment further enhanced triterpenoid production in BpbZIP26 overexpression calli of B. platyphylla using Agrobacterium-mediated transient transformation. Conclusion: This work highlights potential BpbZIP family genes responding to S-nitrosothiol and their roles in triterpenoid production.

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“…The CTAB method was employed to extract the total RNA from the samples [18], followed by treatment with RNase-free DNase I (Takara, San Jose, CA, USA) as per the manufacturer's instructions to eliminate genomic DNA. The concentration and quality of the RNA samples were determined using a DS-11 spectrophotometer (Denovix, Wilmington, DE, USA), assessing both their concentration and 260/280 ratio.…”

Section: Total Rna Extraction and Cdna Synthesismentioning

confidence: 99%

Evaluation of Suitable Reference Genes for Quantitative Real-Time PCR in Various Tissues of Apocynum venetum

Li,

Zhang,

Jiang

et al. 2024

Genes

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Apocynum venetum L. is an economically valuable plant with tolerance to drought and salinity. Its leaves are utilized in tea production and pharmaceuticals, while the stem bark serves as a high-quality fiber material. To gain insights into the gene expression patterns of A. venetum using quantitative real-time PCR (qRT-PCR), it is crucial to identify appropriate reference genes. This study selected nine candidate genes, including α-tubulin (TUA), β-tubulin (TUB), actin (ACT), cyclophilin (CYP), elongation factor-1α (EF-1α), the B family of regulatory subunits of protein phosphatase (PPP2R2, PPP2R3, and PPP2R5), and phosphoglycerate kinase (PGK), to determine the most appropriate reference genes in the leaf, stem, and root tissues of A. venetum. A comprehensive ranking by geNorm, NormFinder, BestKeeper, and RefFinder software and Venn diagrams was used to screen more stable reference genes in different tissues. The two most stable reference genes were CYP and TUA in leaves, PGK and PPP2R3 in stems, and TUA and EF-1α in roots, respectively. The relative expression values of the four genes involved in proline metabolism under polyethylene glycol treatment were used to validate the screened reference genes, and they exhibited highly stable expression levels. These findings represent the first set of stable reference genes for future gene expression studies in A. venetum. They significantly contribute to enhancing the accuracy and reliability of gene expression analyses in this economically important plant species.

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GSNOR deficiency enhances betulin production in Betula platyphylla

Cited by 9 publications

References 36 publications

Characteristic analysis of BpbZIP family genes and BpbZIP26 significantly enhanced triterpenoid production in Betula platyphylla under S-nitrosothiol treatment

Characteristic analysis of BpbZIP family genes and BpbZIP26 significantly enhanced triterpenoid production in Betula platyphylla under S-nitrosothiol treatment

Characteristic analysis of BpbZIP family genes and BpbZIP26 significantly enhanced triterpenoid production in Betula platyphylla under S-nitrosothiol treatment

Evaluation of Suitable Reference Genes for Quantitative Real-Time PCR in Various Tissues of Apocynum venetum

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