GsMATE encoding a multidrug and toxic compound extrusion transporter enhances aluminum tolerance in Arabidopsis thaliana

Ma, Qibin; Rong, Yi; Li, Lü; Liang, Zhongyi; Zeng, Tianyu; Zhang, Yu; Huang, He; Zhang, Xiao; Yin, Xiangli; Cai, Zhandong; Mu, Yinghui; Cheng, Yanbo; Zeng, Qiaoying; Li, Xiuping; Nian, Hai

doi:10.1186/s12870-018-1397-z

Cited by 49 publications

(37 citation statements)

References 63 publications

(102 reference statements)

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“…The purified PCR product was then inserted into the multiple cloning sites of the pLB vector (Tiangen Rapid DNA Ligation Kit, Beijing, China). The positive clones in Escherichia coli were used to obtain the full cDNA sequence of GmWRKY16 identified by PCR, enzyme digestion and sequencing [Sangon Biotech (Shanghai) Co., Ltd., China] (Lü et al, 2015; Ma et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

GmWRKY16 Enhances Drought and Salt Tolerance Through an ABA-Mediated Pathway in Arabidopsis thaliana

et al. 2019

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The WRKY transcription factors (TFs) are one of the largest families of TFs in plants and play multiple roles in plant development and stress response. In the present study, GmWRKY16 encoding a WRKY transcription factor in soybean was functionally characterized in Arabidopsis. GmWRKY16 is a nuclear protein that contains a highly conserved WRKY domain and a C2H2 zinc-finger structure, and has the characteristics of transcriptional activation ability, presenting a constitutive expression pattern with relative expression levels of over fourfold in the old leaves, flowers, seeds and roots of soybean. The results of quantitative real time polymerase chain reaction (qRT-PCR) showed that GmWRKY16 could be induced by salt, alkali, ABA, drought and PEG-6000. As compared with the control, overexpression of GmWRKY16 in Arabidopsis increased the seed germination rate and root growth of seedlings in transgenic lines under higher concentrations of mannitol, NaCl and ABA. In the meantime, GmWRKY16 transgenic lines showed over 75% survival rate after rehydration and enhanced Arabidopsis tolerance to salt and drought with higher proline and lower MDA accumulation, less water loss of the detached leaves, and accumulated more endogenous ABA than the control under stress conditions. Further studies showed that AtWRKY8, KIN1, and RD29A were induced in GmWRKY16 transgenic plants under NaCl treatment. The expressions of the ABA biosynthesis gene (NCED3), signaling genes (ABI1, ABI2, ABI4, and ABI5), responsive genes (RD29A, COR15A, COR15B, and RD22) and stress-related marker genes (KIN1, LEA14, LEA76, and CER3) were regulated in transgenic lines under drought stress. In summary, these results suggest that GmWRKY16 as a WRKY TF may promote tolerance to drought and salt stresses through an ABA-mediated pathway.

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Section: Methodsmentioning

confidence: 99%

GmWRKY16 Enhances Drought and Salt Tolerance Through an ABA-Mediated Pathway in Arabidopsis thaliana

et al. 2019

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“…The inhibition of the root length was an initial detection of Al toxicity in plants (Matsumoto and Motoda, 2012). Plants with strong Al tolerance usually exhibited high relative root growth (Ma et al, 2018;Badia et al, 2020). In this study, the RRG of OE-ZmAT6 transgenic maize was more than 20% higher than those in the WT.…”

Section: Overexpression Of Zmat6 Improved the Al Tolerance Of Transgementioning

confidence: 55%

A Maize ZmAT6 Gene Confers Aluminum Tolerance via Reactive Oxygen Species Scavenging

Huang

et al. 2020

Front. Plant Sci.

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Aluminum (Al) toxicity is the primary limiting factor that affects crop yields in acid soil. However, the genes that contribute to the Al tolerance process in maize are still poorly understood. Previous studies have predicted that ZmAT6 is a novel protein which could be upregulated under Al stress condition. Here, we found that ZmAT6 is expressed in many tissues and organs and can be dramatically induced by Al in both the roots and shoots but particularly in the shoots. The overexpression of ZmAT6 in maize and Arabidopsis plants increased their root growth and reduced the accumulation of Al, suggesting the contribution of ZmAT6 to Al tolerance. Moreover, the ZmAT6 transgenic maize plants had lower contents of malondialdehyde and reactive oxygen species (ROS), but much higher proline content and even lower Evans blue absorption in the roots compared with the wild type. Furthermore, the activity of several enzymes of the antioxidant system, such as peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), increased in ZmAT6 transgenic maize plants, particularly SOD. Consistently, the expression of ZmSOD in transgenic maize was predominant upregulated by Al stress. Taken together, these findings revealed that ZmAT6 could at least partially confer enhanced tolerance to Al toxicity by scavenging ROS in maize.

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“…The full cDNA sequence of GsMAS1 from RT-PCR was 1000 bp with an ORF of 726 bp which encodes 241 amino acids with MW of 27.768 kDa (Additional file 1, Supplementary Materials). The GsMAS1 gene was induced by Al stress (25 µM AlCl 3 , 0.5 mM CaCl 2 , pH 4.3) with over 2-fold expression level from the data of the gene expression profile [89,91]. The Basic Local Alignment Search Tool (BLAST) analysis of NCBI revealed that putative GsMAS1 protein has the structural characteristics of the MADS-box transcription factor with two conservative domains (Additional file 2, Figure S1).…”

Section: Bioinformatics Analysis Of Gsmas1 Genementioning

confidence: 99%

“…All the monoclonal clones were identified by PCR and restriction enzyme digestion. The positive clones were sent to a biotechnology company (Sangon, Shanghai, China) for sequencing, and the sequence result was then confirmed using the NCBI database [91].…”

Section: Construction Of Plant Expression Vectormentioning

confidence: 99%

GsMAS1 Encoding a MADS-box Transcription Factor Enhances the Tolerance to Aluminum Stress in Arabidopsis thaliana

Zhang

Yang

et al. 2020

IJMS

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The MADS-box transcription factors (TFs) are essential in regulating plant growth and development, and conferring abiotic and metal stress resistance. This study aims to investigate GsMAS1 function in conferring tolerance to aluminum stress in Arabidopsis. The GsMAS1 from the wild soybean BW69 line encodes a MADS-box transcription factor in Glycine soja by bioinformatics analysis. The putative GsMAS1 protein was localized in the nucleus. The GsMAS1 gene was rich in soybean roots presenting a constitutive expression pattern and induced by aluminum stress with a concentration-time specific pattern. The analysis of phenotypic observation demonstrated that overexpression of GsMAS1 enhanced the tolerance of Arabidopsis plants to aluminum (Al) stress with larger values of relative root length and higher proline accumulation compared to those of wild type at the AlCl3 treatments. The genes and/or pathways regulated by GsMAS1 were further investigated under Al stress by qRT-PCR. The results indicated that six genes resistant to Al stress were upregulated, whereas AtALMT1 and STOP2 were significantly activated by Al stress and GsMAS1 overexpression. After treatment of 50 μM AlCl3, the RNA abundance of AtALMT1 and STOP2 went up to 17-fold and 37-fold than those in wild type, respectively. Whereas the RNA transcripts of AtALMT1 and STOP2 were much higher than those in wild type with over 82% and 67% of relative expression in GsMAS1 transgenic plants, respectively. In short, the results suggest that GsMAS1 may increase resistance to Al toxicity through certain pathways related to Al stress in Arabidopsis.

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GsMATE encoding a multidrug and toxic compound extrusion transporter enhances aluminum tolerance in Arabidopsis thaliana

Cited by 49 publications

References 63 publications

GmWRKY16 Enhances Drought and Salt Tolerance Through an ABA-Mediated Pathway in Arabidopsis thaliana

GmWRKY16 Enhances Drought and Salt Tolerance Through an ABA-Mediated Pathway in Arabidopsis thaliana

A Maize ZmAT6 Gene Confers Aluminum Tolerance via Reactive Oxygen Species Scavenging

GsMAS1 Encoding a MADS-box Transcription Factor Enhances the Tolerance to Aluminum Stress in Arabidopsis thaliana

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