GSK3/shaggy-like kinase 1 ubiquitously regulates cell growth from Arabidopsis to Moso bamboo (Phyllostachys edulis)

Wang, Taotao; Li, Qinzhen; Lou, Shuaitong; Yang, Yong; Lingfang, Peng; Lin, Zezhong; Hu, Qin; Ma, Liuyin

doi:10.1016/j.plantsci.2019.03.015

Cited by 18 publications

(22 citation statements)

References 45 publications

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“…Therefore, we hypothesized that PeGATA26 plays a crucial role in regulating plant growth. In a previous study, we successfully characterized one of the fast growth-suppressing genes PeGSK1 by over-expressing it into Arabidopsis [1]. Therefore, we used a similar strategy to verify the function of PeGATA26 in regulating plant growth by over-expressing it into Arabidopsis.…”

Section: Resultsmentioning

confidence: 99%

“…To verify the location of PeGATAs, the full-length CDSs without stop codon from four PeGATA genes were cloned into a modified pCambia3301 vector with C-terminal GFP as described in our previous study [1]. The ACTIN2::PeGATAs -GFP and the ACTIN2 ::GFP control constructs were then transiently transformed into tobaccos, and GFP and DAPI fluorescence was observed using a microscope (20x, Zeiss, LSM880).…”

Section: Methodsmentioning

confidence: 99%

“…The PeGATA26 was cloned and expressed in Arabidopsis exactly following the procedures for the PeGSKl in our previous study[1]. The T3 generation seedlings were used for phenotype analysis.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Moso bamboo is one of most-abundant non-timber forestry species and provides important resources for food, architecture, papermaking and fiber [1]. More importantly, moso bamboo is known for its explosive shoot growth rate, with a peak growth rate of 1 meter per day [1]. The rapid-growth shoot is largely dependent on the widespread rhizome system, which provide energy resources by absorbing from soil and more importantly, transporting from other rhizome-connected adult bamboos [2].…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide analysis of GATA factors in moso bamboo (Phyllostachys edulis) unveils that PeGATAs regulate shoot rapid-growth and rhizome development

Wang

Yang

Lou

et al. 2019

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18 · Background 19Moso bamboo is well-known for its rapid-growth shoots and widespread rhizomes. 20However, the regulatory genes of these two processes are largely unexplored. 21 GATA factors regulate many developmental processes, but its role in plant height 22 control and rhizome development remains unclear. 23 · Results 24Here, we found that bamboo GATA factors (PeGATAs) are involved in the 25 growth regulation of bamboo shoots and rhizomes. Bioinformatics and 26 evolutionary analysis showed that there are 31 PeGATA factors in bamboo, which 27 can be divided into three subfamilies. Light, hormone, and stress-related 28 cis-elements were found in the promoter region of the PeGATA genes. Gene 29 expression of 12 PeGATA genes was regulated by phytohormone-GA but there 30 was no correlation between auxin and PeGATA gene expression. More than 27 31PeGATA genes were differentially expressed in different tissues of rhizomes, and 32 almost all PeGATAs have dynamic gene expression level during the rapid-growth 33 3 of bamboo shoots. These results indicate that PeGATAs regulate rhizome 34 development and bamboo shoot growth partially via GA signaling pathway. In 35 addition, PeGATA26, a rapid-growth negative regulatory candidate gene 36 modulated by GA treatment, was overexpressed in Arabidopsis, and 37 over-expression of PeGATA26 significantly repressed Arabidopsis primary root 38 length and plant height. The PeGATA26 overexpressing lines were also resistant 39 to exogenous GA treatment, further emphasizing that PeGATA26 inhibits plant 40 height from Arabidopsis to moso bamboo via GA signaling pathway. 41 · Conclusions 42Our results provide an insight into the function of GATA transcription factors in 43 regulating shoot rapid-growth and rhizome development, and provide genetic 44 resources for engineering plant height. 45Running title: GATA family in moso bamboo 46

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%