GSK3 and KIF5 regulate activity-dependent sorting of gephyrin between axons and dendrites

Rathgeber, Louisa; Gromova, Kira V.; Schaefer, Irina; Breiden, Petra; Lohr, Christian; Kneussel, Matthias

doi:10.1016/j.ejcb.2015.01.005

Cited by 8 publications

(7 citation statements)

References 36 publications

(56 reference statements)

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“…This supports the hypothesis that the KIF5B and KIF5C motor domains inherently confer axon‐selective transport in the absence of on‐vesicle regulation . Among the vesicles labeled in dendrites could be the previously proposed Kinesin‐1‐mediated dendrite‐selective carrriers that transport AMPA receptors . This observation may suggest that dendritic transport by Kinesin‐1 requires on‐vesicle regulatory mechanisms …”

Section: Resultssupporting

confidence: 82%

A novel strategy to visualize vesicle‐bound kinesins reveals the diversity of kinesin‐mediated transport

Yang

Bostick

Garbouchian

et al. 2019

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In mammals, 15 to 20 kinesins are thought to mediate vesicle transport. Little is known about the identity of vesicles moved by each kinesin or the functional significance of such diversity. To characterize the transport mediated by different kinesins, we developed a novel strategy to visualize vesicle‐bound kinesins in living cells. We applied this method to cultured neurons and systematically determined the localization and transport parameters of vesicles labeled by different members of the Kinesin‐1, ‐2, and ‐3 families. We observed vesicle labeling with nearly all kinesins. Only six kinesins bound vesicles that undergo long‐range transport in neurons. Of these, three had an axonal bias (KIF5B, KIF5C and KIF13B), two were unbiased (KIF1A and KIF1Bβ), and one transported only in dendrites (KIF13A). Overall, the trafficking of vesicle‐bound kinesins to axons or dendrites did not correspond to their motor domain preference, suggesting that on‐vesicle regulation is crucial for kinesin targeting. Surprisingly, several kinesins were associated with populations of somatodendritic vesicles that underwent little long‐range transport. This assay should be broadly applicable for investigating kinesin function in many cell types.

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Section: Resultssupporting

confidence: 82%

A novel strategy to visualize vesicle‐bound kinesins reveals the diversity of kinesin‐mediated transport

Yang

Bostick

Garbouchian

et al. 2019

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“…Such MBOs include mitochondria, and tubulo-vesicular MBOs containing amyloid precursor protein (APP), neurotransmitter receptors, plasmalemma and synaptic vesicle precursors [59,96,98]. Antisense oligonucleotide experiments in cultured neurons confirmed a major role of conventional kinesin on the AT of membrane proteins, including APP, to axons and dendrites [25,86], but these experiments failed to reveal the issue of biochemical diversity.…”

Section: Functional Implications Of Conventional Kinesin Subunit Varimentioning

confidence: 94%

Conventional kinesin: Biochemical heterogeneity and functional implications in health and disease

Morfini

Schmidt

Weissmann

et al. 2016

Brain Research Bulletin

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“…KIF5 also transports Gephyrin, a scaffold protein specific at the inhibitory synapse, in an activity-dependent manner. Gephyrin acts as the adaptor between KIF5 and glycine receptor [46,47]. Blockade of glycine receptor reduces the mobility of KIF5 and gephyrin particles without affecting the KIF5-mediated transport of GRIP1, suggesting a cargo/adaptor-specific regulatory mechanism [46].…”

Section: Transport Of Inhibitory Neurotransmitter Receptormentioning

confidence: 99%

“…Blockade of glycine receptor reduces the mobility of KIF5 and gephyrin particles without affecting the KIF5-mediated transport of GRIP1, suggesting a cargo/adaptor-specific regulatory mechanism [46]. Interestingly, a later study reported that KIF5 is responsible for the mis-sorting of gephyrin into axon upon chronic AMPA receptor activation [47]. Whether gephyrin has a physiological role in axon and how is this activity-dependent sorting mechanism related to neuronal function remain to be determined.…”

Section: Transport Of Inhibitory Neurotransmitter Receptormentioning

confidence: 99%

Understanding how kinesin motor proteins regulate postsynaptic function in neuron

Fan

Lai

2021

The FEBS Journal

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The Kinesin superfamily proteins (KIFs) are major molecular motors that transport diverse set of cargoes along microtubules to both the axon and dendrite of a neuron. Much of our knowledge about kinesin function is obtained from studies on axonal transport. Emerging evidence reveals how specific kinesin motor proteins carry cargoes to dendrites, including proteins, mRNAs and organelles that are crucial for synapse development and plasticity. In this review, we will summarize the major kinesin motors and their associated cargoes that have been characterized to regulate postsynaptic function in neuron. We will also discuss how specific kinesins are selectively involved in the development of excitatory and inhibitory postsynaptic compartments, their regulation by post-translational modifications (PTM), as well as their roles beyond conventional transport carrier.

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GSK3 and KIF5 regulate activity-dependent sorting of gephyrin between axons and dendrites

Cited by 8 publications

References 36 publications

A novel strategy to visualize vesicle‐bound kinesins reveals the diversity of kinesin‐mediated transport

A novel strategy to visualize vesicle‐bound kinesins reveals the diversity of kinesin‐mediated transport

Conventional kinesin: Biochemical heterogeneity and functional implications in health and disease

Understanding how kinesin motor proteins regulate postsynaptic function in neuron

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