gSG6-P1 salivary biomarker discriminates micro-geographical heterogeneity of human exposure to Anopheles bites in low and seasonal malaria areas

Sagna, Aloïse; Sarr, Joseph; Gaayeb, Lobna; Drame, Papa M.; Ndiath, Mamadou Ousmane; Senghor, Simon; Sow, Cheikh; Poinsignon, Anne; Seck, Modou; Hermann, Emmanuel; Schacht, Anne‐Marie; Faye, N.; Sokhna, Cheikh; Remoué, Franck; Riveau, Gilles

doi:10.1186/1756-3305-6-68

Cited by 43 publications

(75 citation statements)

References 49 publications

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“…In this paper we have used an “ in silico ” approach to select candidate peptides within the sequence of our candidate proteins as experimental methods used for characterizing epitopes are time consuming and demand large resources. A similar approach has previously been successfully applied to design the gSG6-P1 peptide [33], [34], [52] from the gSG6 protein, a small anopheline-specific salivary protein [31]. In our study, out of only three synthetic peptides selected by bioinformatics tools, one was a good candidate characterized by a good antigenicity and specificity confirming further the validity of this approach.…”

Section: Discussionsupporting

confidence: 73%

In Silico Identification of a Candidate Synthetic Peptide (Tsgf118–43) to Monitor Human Exposure to Tsetse Flies in West Africa

et al. 2013

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BackgroundThe analysis of humoral responses directed against the saliva of blood-sucking arthropods was shown to provide epidemiological biomarkers of human exposure to vector-borne diseases. However, the use of whole saliva as antigen presents several limitations such as problems of mass production, reproducibility and specificity. The aim of this study was to design a specific biomarker of exposure to tsetse flies based on the in silico analysis of three Glossina salivary proteins (Ada, Ag5 and Tsgf1) previously shown to be specifically recognized by plasma from exposed individuals.Methodology/Principal FindingsSynthetic peptides were designed by combining several linear epitope prediction methods and Blast analysis. The most specific peptides were then tested by indirect ELISA on a bank of 160 plasma samples from tsetse infested areas and tsetse free areas. Anti-Tsgf118–43 specific IgG levels were low in all three control populations (from rural Africa, urban Africa and Europe) and were significantly higher (p<0.0001) in the two populations exposed to tsetse flies (Guinean HAT foci, and South West Burkina Faso). A positive correlation was also found between Anti-Tsgf118–43 IgG levels and the risk of being infected by Trypanosoma brucei gambiense in the sleeping sickness foci of Guinea.Conclusion/SignificanceThe Tsgf118–43 peptide is a suitable and promising candidate to develop a standardize immunoassay allowing large scale monitoring of human exposure to tsetse flies in West Africa. This could provide a new surveillance indicator for tsetse control interventions by HAT control programs.

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Section: Discussionsupporting

confidence: 73%

In Silico Identification of a Candidate Synthetic Peptide (Tsgf118–43) to Monitor Human Exposure to Tsetse Flies in West Africa

et al. 2013

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“…26 The area is located in a dry savannah with a dry season from November to June and a short rainy season from July to October. 26, 27 In this region, malaria due to P. falciparum shows a low and seasonal incidence, where peaks of infections appear after the rainy season. 28 Anopheles gambiae s.l.…”

Section: Methodsmentioning

confidence: 99%

“…is the major malaria vector in the Podor District. 26, 28 Samples were collected from the Niandane village in the month of January 2009, after the rainy season when a peak of malaria transmission by An. gambiae s.l.…”

Section: Methodsmentioning

confidence: 99%

“…was recorded. 26 Samples from infected ( N =22) and uninfected ( N =26) children were included in the study (Supplementary Table 5). Serum samples from patients with untreated pulmonary tuberculosis or during the first week of treatment ( N =41) and uninfected healthy individuals ( N =43) were obtained from adults in the Iberian Peninsula (Portugal and Spain) (Supplementary Table 6).…”

Section: Methodsmentioning

confidence: 99%

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Effect of blood type on anti-α-Gal immunity and the incidence of infectious diseases

et al. 2017

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The identification of factors affecting the susceptibility to infectious diseases is essential toward reducing their burden on the human population. The ABO blood type correlates with susceptibility to malaria and other infectious diseases. Due to the structural similarity between blood antigen B and Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal), we hypothesized that self-tolerance to antigen B affects the immune response to α-Gal, which in turn affects the susceptibility to infectious diseases caused by pathogens carrying α-Gal on their surface. Here we found that the incidence of malaria and tuberculosis, caused by pathogens with α-Gal on their surface, positively correlates with the frequency of blood type B in endemic regions. However, the incidence of dengue fever, caused by a pathogen without α-Gal, was not related to the frequency of blood type B in these populations. Furthermore, the incidence of malaria and tuberculosis was negatively correlated with the anti-α-Gal antibody protective response. These results have implications for disease control and prevention.

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“…Antibody reactivity to this peptide shows promising characteristics as a biomarker for human biting; increases in these specific antibody levels correlated with increased rainfall in a region of very low mosquito exposure and rapid decreases in these levels were observed in individuals after ITNs were introduced in areas of high malaria transmission. [29][30][31] At this point, there is no ability to distinguish biting by different Anopheles species, although this would be important for estimating the relative contribution of each species to transmission. More information on the sensitivity of this assay also would be helpful, with regard to the number of bites required to elicit a measurable response.…”

Section: Priorities and Progressmentioning

confidence: 99%

Needs for Monitoring Mosquito Transmission of Malaria in a Pre-Elimination World

James

Takken

Collins

et al. 2014

The American Journal of Tropical Medicine and Hygiene

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Abstract. As global efforts to eliminate malaria intensify, accurate information on vector populations and transmission dynamics is critical for directing control efforts, developing new control tools, and predicting the effects of these interventions under various conditions. Currently available sampling tools for mosquito population monitoring suffer from well-recognized limitations. As reported in this workshop summary, a recent gathering of medical entomologists, modelers, and malaria experts reviewed these issues and agreed that efforts are needed to improve methods to monitor key transmission parameters. Identified needs include standardized methods for sampling of both mosquito adults and larvae, improved tools for mosquito species identification and age-grading, and a better means for determining the entomological inoculation rate.

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gSG6-P1 salivary biomarker discriminates micro-geographical heterogeneity of human exposure to Anopheles bites in low and seasonal malaria areas

Cited by 43 publications

References 49 publications

In Silico Identification of a Candidate Synthetic Peptide (Tsgf118–43) to Monitor Human Exposure to Tsetse Flies in West Africa

In Silico Identification of a Candidate Synthetic Peptide (Tsgf118–43) to Monitor Human Exposure to Tsetse Flies in West Africa

Effect of blood type on anti-α-Gal immunity and the incidence of infectious diseases

Needs for Monitoring Mosquito Transmission of Malaria in a Pre-Elimination World

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