Grünlicht‐induzierte Rezeptorinaktivierung durch Cobalamin‐bindende Domänen

Abstract

[3] oder Oligomerisierung von CRY.[4] Des Weiteren wurde Blaulicht-oder Rotlicht-induziertes Dissoziieren von Proteinkomplexen, die sich spontan in Dunkelheit bilden, fürLOV-Domänen in vitro [5] und fürPHY und CRYin Hefe-Screens [6] beobachtet und im Fall von LOV-Domänen und manipulierten fluoreszierenden Proteinen als optogenetisches Verfahren implementiert. [7] Keine der bisher verwendeten optogenetischen Methoden zeigt jedoch ein Maximum an Empfindlichkeit gegenüber grünem Licht, das daher einen "blinden Fleck" in optogenetischen Experimenten darstellt (Abbildung 1a). Hier nutzen wir Cobalamin(Vitamin B12)-

Engineered Illumination Devices for Optogenetic Control of Cellular Signaling Dynamics

“…In particular, a variety of photosensory domains have been discovered, optimized, and repurposed to place intracellular signaling pathways under light con-trol, capabilities that offer the unique advantage of using light patterns to stimulate signaling in a specific location and at a specific time (Repina et al, 2017). Within mammalian cells, optogenetic tools that control protein-protein interactions have, for example, been developed for diverse signaling pathways such as Wnt/b-catenin (b-CAT) (Bugaj et al, 2013;Repina et al, 2019), Ras/Raf/Mek/Erk (Toettcher et al, 2011;Johnson and Toettcher, 2019;Toettcher et al, 2013;Johnson et al, 2017), fibroblast growth factor (FGF) (Kainrath et al, 2017), receptor tyrosine kinases (Bugaj et al, 2015), and Rho-family guanosine triphosphatase (GTPase) signaling (Levskaya et al, 2009;Yazawa et al, 2009;Strickland et al, 2012;Guntas et al, 2015;Bugaj et al, 2013;Wang et al, 2010). Optogenetic methods have also been applied recently to studies in developmental biology in key model organisms (Johnson and Toettcher, 2018).…”

Optogenetics: A Primer for Chemists

“…The photolytic loss of the adenosyl moiety generates a cavity that is filled by a nearby tryptophan residue, which results in a massive conformational change that drives the tetramer‐to‐dimer/monomer transition . Finally, although a wide variety of optogenetic constructs have been described containing the antennas discussed in Sections 2.1.–2.5., the introduction of a B 12 ‐dependent optogenetic species is a recent development …”