Grundlegende Versuche zur Keimungsphysiologie von Pilzsporen

Sommer, Liesel; Halbsguth, Wilhelm

doi:10.1007/978-3-663-04860-2

Cited by 22 publications

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“…5 min at 50 "C (Sommer & Halbsguth, 1957) or 15 min in 0.1 Mammonium acetate (Borchert, 1962). One of the main features of early germination is a temporary increase in cyclic AMP content (Van Mulders & Van Laere, 1984) correlated with a substantial conversion of reserve trehalose to glycerol (Van Schaftingen & Van Laere, 1985) which greatly affects the water status of the spores (Van Laere et al, Van Laere & Hulsmans, 1987).…”

Section: Introductionmentioning

confidence: 99%

Purification and Properties of NAD-dependent Glutamate Dehydrogenase from Phycomyces Spores

Laere

1988

Microbiology

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The NAD-dependent glutamate dehydrogenase from Phycomyces spores was purified more than 300-fold. Estimation of Mr by gel filtration gave a value of 98,000 whereas after SDS-PAGE one major band of Mr 54,000 was found, suggesting that the enzyme is a dimer. The enzyme was virtually dependent on the presence of AMP for activity and showed half-maximal activation at 9.5 and 43 microM-AMP in the direction of animation and deamination respectively. ADP was nearly as effective at 20-fold higher concentrations. Other nucleotide monophosphates were ineffective and nucleoside triphosphates were slightly inhibitory. Hyperbolic kinetics were found for all substrates yielding Km values of about 10 mM for ammonium, 1 mM for 2-oxoglutarate and 0.1 mM for NADH in the direction of amination, and 10 mM for glutamate and 0.7 mM for NAD in the direction of deamination.

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Section: Introductionmentioning

confidence: 99%

Purification and Properties of NAD-dependent Glutamate Dehydrogenase from Phycomyces Spores

Laere

1988

Microbiology

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“…Without activation, germination of Phycomyces sporangiospores in a complete culture medium is limited to some 3 % of the population (Sommer & Halbsguth, 1957). The constitutively dormant spores can be activated by several treatments, heating for some minutes at 45 to 50 "C or treatment with acetate (Robbins et al, 1942) being most reliable.…”

Section: Introductionmentioning

confidence: 99%

Pyruvate Metabolism by Mitochondria from Dormant and Activated Phycomyces blakesleeanus Spores

1982

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~~Activation of Phycomyces blakesleeanus spores resulted in the production of large amounts of pyruvate and 2-oxoglutarate. Incubation of dormant spores in 0.1 M-pyruvate at pH 3 resulted in a high internal pyruvate concentration but no 2-oxoglutarate was formed and neither germination nor respiration was stimulated. The capacity of isolated mitochondria to decarboxylate [ l-'4C]pyruvate was doubled by heat activation of the spores. This difference in activity disappeared when the mitochondria were subjected to treatments attacking the integrity of the mitochondrial membrane (detergent, resuspension in buffer without osmotic stabilizer). The increase in pyruvate decarboxylating activity was found only after heating the spores at temperatures also triggering germination of the spores. Pyruvate uptake by the mitochondria seems, therefore, to be a limiting factor in dormant spore metabolism.

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“…However, there are some thiamin-auxotrophic yeasts among the Saccharomycetes that lack THI5p (Wightman and Meacock 2003) and various species in other lines of Ascomycetes show also deficiencies in thiamin production (Fries 1943;Bereston 1953). As early as in the first half of the last century it had been established that many lines of Basidiomycota and also of the Mucoromycotina are auxotrophic for thiamin and do not grow or grow only very reluctantly without addition of thiamin (Burgeff 1934;Schopfer 1937Schopfer , 1943Bonner and Erickson 1938;Fries 1938;Melin 1939;Melin and Nyman 1940;Schopfer and Blumer 1940;Kühlwein and Zoberst 1953;Lyr 1954;Madelin 1956;Sommer and Halbsguth 1957;Rawald 1962;Volz and Beneke 1969;Volz 1972;Eul and Schwantes 1985;Gramss 1990;Beguin 2010). In Agaricomycetes, also fruiting body development can negatively be affected by lack of sufficient thiamin in a culture medium .…”

Section: Introductionmentioning

confidence: 99%

Factors Influencing Fruiting Body Initation of Agaricomycetes

Reddy¹

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Grundlegende Versuche zur Keimungsphysiologie von Pilzsporen

Cited by 22 publications

References 0 publications

Purification and Properties of NAD-dependent Glutamate Dehydrogenase from Phycomyces Spores

Purification and Properties of NAD-dependent Glutamate Dehydrogenase from Phycomyces Spores

Pyruvate Metabolism by Mitochondria from Dormant and Activated Phycomyces blakesleeanus Spores

Factors Influencing Fruiting Body Initation of Agaricomycetes

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