Grundlagen der Adsorption

Bathen, Dieter; Breitbach, Marc

doi:10.1007/978-3-642-18235-8_3

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…GSSG adsorption was rapid as demonstrated by maximal bindings that were achieved within 5 min, and the adsorption isotherms demonstrated that the GSSG adsorption was saturable. The Gibbs energy of adsorption Δ G ads 0 estimated from the GSSG adsorption isotherms were in the range of typical values for electrostatic adsorption processes (<50 kJ/mol) and similar to those previously found for GSSG and GSH adsorption onto alumina. , The negative values of Δ G ads 0 , which were almost identical for Al 2 O 3 , Al 2 O 3 –NH 2 , or Al 2 O 3 –SO 3 H particles, indicate an exergonic adsorption behavior of GSSG to all three types of particles, which thus appears in this regard to be rather independent of the surface functionalization.…”

Section: Discussionsupporting

confidence: 85%

Adsorption and Orientation of the Physiological Extracellular Peptide Glutathione Disulfide on Surface Functionalized Colloidal Alumina Particles

et al. 2013

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Understanding the interrelation between surface chemistry of colloidal particles and surface adsorption of biomolecules is a crucial prerequisite for the design of materials for biotechnological and nanomedical applications. Here, we elucidate how tailoring the surface chemistry of colloidal alumina particles (d50 = 180 nm) with amino (-NH2), carboxylate (-COOH), phosphate (-PO3H2) or sulfonate (-SO3H) groups affects adsorption and orientation of the model peptide glutathione disulfide (GSSG). GSSG adsorbed on native, -NH2-functionalized, and -SO3H-functionalized alumina but not on -COOH- and -PO3H2-functionalized particles. When adsorption occurred, the process was rapid (≤5 min), reversible by application of salts, and followed a Langmuir adsorption isotherm dependent on the particle surface functionalization and ζ potential. The orientation of particle bound GSSG was assessed by the release of glutathione after reducing the GSSG disulfide bond and by ζ potential measurements. GSSG is likely to bind via the carboxylate groups of one of its two glutathionyl (GS) moieties onto native and -NH2-modified alumina, whereas GSSG is suggested to bind to -SO3H-modified alumina via the primary amino groups of both GS moieties. Thus, GSSG adsorption and orientation can be tailored by varying the molecular composition of the particle surface, demonstrating a step toward guiding interactions of biomolecules with colloidal particles.

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