Grp1-associated scaffold protein regulates skin homeostasis after ultraviolet irradiation

Abstract

Grp1-associated scaffold protein (Grasp), the product of a retinoic acid-induced gene in P19 embryonal carcinoma cells, is expressed primarily in brain, heart, and lung of the mouse. We report herein that Grasp transcripts are also found in mouse skin in which the Grasp gene is robustly induced following acute ultraviolet-B (UVB) exposure. Grasp−/− mice were found to exhibit delayed epidermal proliferation and a blunted apoptotic response after acute UVB exposure. Immunohistochemical analyses revealed that the nuclear residence time of the tumor suppressor protein p53 was reduced in Grasp−/− mice after UVB exposure. Taken together, our results suggest that a physiological role of Grasp may be to regulate skin homeostasis after UVB exposure, potentially by influencing p53-mediated apoptotic responses in skin.

Accurate diagnosis of atopic dermatitis by combining transcriptome and microbiota data with supervised machine learning

“…Hence, we perform a literature search for these 19 genes to examine whether they are known to be related to AD and find a few cases. First, we find that GRP1 (Probe ID: 16907572) is associated with a type of scaffold protein (Grasp) that potentially influences p53-mediated apoptotic responses in the skin 33 . It is known that apoptosis is a crucial process in the development of AD 34 , 35 .…”

Identification of Core Genes and Key Pathways via Integrated Analysis of Gene Expression and DNA Methylation Profiles in Bladder Cancer

“…There are various scaffold proteins exerting different functions in carcinogenesis. Grp1-associated scaffold protein promotes the function of p53 tumor suppressor genes and inhibits cancer development [ 39 ]. In our study, we found that many hypermethylated/low-expression genes were enriched in scaffold protein binding.…”