The success of shallot cultivation in vertisol soil is influenced by the use of seeds and the application of organic matter. This aim of this study aims to determine was to the effect of local organic matter on vertisol soil on the growth and yield of three true shallot seed (TSS) varieties. The research was carried out in Plembutan, Playen, Gunung Kidul. The research was conducted from May-August 2021. The research method used a Split Plot Design with 3 replications. The main plot consists of types of local organic matter (B), consisting of B1 = Cow, and B2 = Goat, B3 = Chicken. While the sub-plots were Varieties (V), consisting of: V1 = Maserati, V2 = Sanren, and V3 = Lokananta. Plant height, number of leaves, number of bulbs per clump, dry bulb weight per clump, bulb diameter, and productivity were all measured. The data was analyzed using variance and DMRT 5%.The results showed that the type of local organic matter did not significantly interact with the TSS variety. Plant height, number of leaves, number of tubers per clump, dry tuber weight per clump, tuber diameter, and productivity were not affected by the organic matter and variety treatments. The type of local organic matter from cows produces the highest growth and yields, followed by goats and chickens. The lokananta variety produced the highest productivity, followed by Sanren and Maserati.