Growth techniques

Hui, Colleen; Schmollinger, Stefan; Glaesener, Anne G.

doi:10.1016/b978-0-12-822457-1.00005-4

Cited by 4 publications

(2 citation statements)

References 206 publications

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“…Light intensity was either ~100 µmol photons m -2 s -1 (increased light, standard lab culturing conditions; Hui et al 2023) or ~10 µmol photons m -2 s -1 (low light, equivalent to light levels at ~15-m depth in Lake Bonney; Lizotte and Priscu 1992). Light intensity was measured with a quantum sensor attached to a radiometer (Model LI-189; Li-COR).…”

Section: Methodsmentioning

confidence: 99%

Light quality affects chlorophyll biosynthesis and photosynthetic performance in Antarctic Chlamydomonas

Poirier,

Fugard,

Cvetkovska

2024

Preprint

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The perennially ice-covered Lake Bonney in Antarctica has been deemed a natural laboratory for studying life at the extreme. Photosynthetic algae dominate the lake food webs and are adapted to a multitude of extreme conditions including perpetual shading even at the height of the austral summer. Here we examine how the unique light environment in Lake Bonney influences the physiology of twoChlamydomonasspecies.Chlamydomonas priscuiiis found exclusively in the deep photic zone where is receives very low light levels biased in the blue part of the spectrum (400-500 nm). In contrast,Chlamydomonassp. ICE-MDV is represented at various depths within the water column (including the bright surface waters), and it receives a broad range of light levels and spectral wavelengths. The close phylogenetic relationship and psychrophilic character of both species makes them an ideal system to study the effects of light quality and quantity on chlorophyll biosynthesis and photosynthetic performance in extreme conditions. We show that the shade-adaptedC. priscuiiexhibits a decreased ability to accumulate chlorophyll and severe photoinhibition when grown under red light compared to blue light. These effects are particularly pronounced under red light of higher intensity, suggesting a loss of capability to acclimate to varied light conditions. In contrast, ICE-MDV has retained the ability to synthesize chlorophyll and maintain photosynthetic efficiency under a broader range of light conditions. Our findings provide insights into the mechanisms of photosynthesis under extreme conditions, and have implications on algal survival in changing conditions of Antarctic ice-covered lakes.

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Section: Methodsmentioning

confidence: 99%

Light quality affects chlorophyll biosynthesis and photosynthetic performance in Antarctic Chlamydomonas

Poirier,

Fugard,

Cvetkovska

2024

Preprint

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“…We have developed this alga as a useful system for investigating trace metal homeostasis (Blaby-Haas & Merchant, 2012, 2023; Glaesener et al, 2013; Merchant et al, 2006). It is easy to manipulate the Fe content within the defined growth medium and homogenously supply Fe to cells without variations in organ, tissue, or cell type (Hui et al 2023). Another strength of Chlamydomonas for metal homeostasis is that nutritional deficiency is possible, in contrast to other systems that rely on chelator-imposed deficiency.…”

Section: Introductionmentioning

confidence: 99%

Chlamydomonas cells transition through distinct Fe nutrition stages within 48 h of transfer to Fe-free medium

Liu,

Urzica,

Gallaher

et al. 2024

Preprint

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Low iron (Fe) bioavailability can limit the biosynthesis of Fe-containing proteins, which are especially abundant in photosynthetic organisms, thus negatively affecting global primary productivity. Understanding cellular coping mechanisms under Fe limitation is therefore of great interest. We surveyed the temporal responses of Chlamydomonas (Chlamydomonas reinhardtii) cells transitioning from an Fe-rich to an Fe-free medium to document their short- and long-term adjustments. While slower growth, chlorosis and lower photosynthetic parameters are evident only after one or more days in Fe-free medium, the abundance of some transcripts, such as those for genes encoding transporters and enzymes involved in Fe assimilation, change within minutes, before changes in intracellular Fe content are noticeable, suggestive of a sensitive mechanism for sensing Fe. Promoter reporter constructs indicate a transcriptional component to this immediate primary response. With acetate provided as a source of reduced carbon, transcripts encoding respiratory components are maintained relative to transcripts encoding components of photosynthesis and tetrapyrrole biosynthesis, indicating metabolic prioritization of respiration over photosynthesis. In contrast to the loss of chlorophyll, carotenoid content is maintained under Fe limitation despite a decrease in the transcripts for carotenoid biosynthesis genes, indicating carotenoid stability. These changes occur more slowly, only after the intracellular Fe quota responds, indicating a phased response in Chlamydomonas, involving both primary and secondary responses during acclimation to poor Fe nutrition.

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High-Throughput Screening to Accelerate Microalgae-Based Phycochemical Production

Wolf,

Chapman,

Deepika

et al. 2023

Value-Added Products From Algae

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Growth techniques

Cited by 4 publications

References 206 publications

Light quality affects chlorophyll biosynthesis and photosynthetic performance in Antarctic Chlamydomonas

Light quality affects chlorophyll biosynthesis and photosynthetic performance in Antarctic Chlamydomonas

Chlamydomonas cells transition through distinct Fe nutrition stages within 48 h of transfer to Fe-free medium

High-Throughput Screening to Accelerate Microalgae-Based Phycochemical Production

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