Growth-suppressive effect of berberine on endometrial carcinoma cells: Role of mitochondrial and PI3K/Akt pathway

Kuo, Han Peng; Lee, Yi Jen; Hsu, Cheng-Ying; Lee, Shou Lun; Hsu, Shih Chung; Chuang, Tzu Chao; Liu, Jah Yao; Kuo, Chao Lin; Ho, Chi‐Tang; Kao, Ming‐Ching

doi:10.1016/j.jff.2015.06.006

Cited by 12 publications

(8 citation statements)

References 30 publications

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“…PI3K/AKT signaling was an important pathway in cellular biology that regulated cell proliferation, cell cycle progression, and apoptosis (Chan et al, ; Kuo et al, ). In this study, miR‐26a did not affect the expression of total AKT protein levels (Figure a), but it increased the p‐AKT (phosphorylated AKT) levels at 48 hr in EECs, and the miR‐26a inhibitor decreased p‐AKT levels (Figure b).…”

Section: Resultsmentioning

confidence: 99%

miR‐26a promoted endometrial epithelium cells (EECs) proliferation and induced stromal cells (ESCs) apoptosis via the PTEN‐PI3K/AKT pathway in dairy goats

Zhang

Liu

et al. 2018

Journal Cellular Physiology

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Changes in endometrial cell morphology and function are absolutely necessary for successful embryo implantation. In this study, miR-26a was widely expressed in dairy goats, and was found to be regulated by β-estradiol (E2) and progesterone (P4) in endometrial epithelium cells (EECs) as well as stromal cells (ESCs). Furthermore, miR-26a played a role in the regulation of cells proliferation and apoptosis by directly regulating PTEN and indirectly regulating the PI3K/AKT pathway in EECs but not in ESCs of dairy goats in vitro. In addition, miR-26a regulated the expression of osteopontin (OPN), vascular endothelial growth factor (VEGF), Cyclooxygenase-2 (COX-2), and prolactin (PRL) in endometrial cells. Therefore, we could get a conclusion that miR-26a had very complex and diverse functions in the endometrial cells during the development of endometrial receptivity in dairy goats. This study provided an efficient platform for studying the regulatory effect of miR-26a on endometrial cells during the development of endometrial receptivity in dairy goats.

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Section: Resultsmentioning

confidence: 99%

miR‐26a promoted endometrial epithelium cells (EECs) proliferation and induced stromal cells (ESCs) apoptosis via the PTEN‐PI3K/AKT pathway in dairy goats

Zhang

Liu

et al. 2018

Journal Cellular Physiology

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“…Cell proliferation is generally regulated by progression through the cell cycle [42]. Consequently, disruption of the cell cycle is considered the common cause behind the inhibition of cell proliferation [43].…”

Section: Resultsmentioning

confidence: 99%

“…In this study, MTT assay (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) showed that miR-182 mimics inhibited cell proliferation and miR-182 inhibitors promoted cell proliferation, but the differences were not statistically significant in EEC cells ( Fig 5 ). Cell proliferation is generally regulated by progression through the cell cycle [ 42 ]. Consequently, disruption of the cell cycle is considered the common cause behind the inhibition of cell proliferation [ 43 ].…”

Section: Resultsmentioning

confidence: 99%

“…In his study, an MTT assay showed that the miR-182 mimics inhibited and miR-182 inhibitors promoted the proliferation of EECs ( Fig 5 ), although the differences were not significant (P > 0.05). Cell proliferation is generally regulated by progression through the cell cycle [ 42 ]. Consequently, the disruption of the cell cycle is considered to be a common cause of cell proliferation inhibition [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

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miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression

Zhang

Liu

et al. 2017

PLoS ONE

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Increasing evidence has shown that miRNAs play important roles in endometrium development during the menstrual cycle in humans and many other animals. Our previous data indicated that miR-182 levels increase 15.55-fold and pleiotrophin (PTN) levels decrease 20.97-fold in the receptive endometrium (RE, D15) compared with the pre-receptive endometrium (PE, D5) in dairy goats. The present study shows that miR-182 is widely expressed in different tissues of dairy goats and that its expression levels are regulated by E2 and P4 in endometrial epithelium cells (EECs). We confirmed that PTN is a target of miR-182 and that miR-182 regulates the protein levels of AKT, Bcl-2, FAS, MAPK, Caspase-3 and SP1 in EECs. Furthermore, miR-182 up-regulates or maintains the expression levels of osteopontin (OPN), cyclooxygenase-2 (COX-2) and prolactin receptor (PRLR) in EECs, suggesting that miR-182 is an important regulatory factor in the construction of endometrial receptivity in dairy goats. In conclusion, miR-182 participates in the development of endometrial receptivity by down-regulating PTN and affecting the expression of select apoptosis-related genes and increasing or maintaining the expression levels of OPN, COX-2 and PRLR in the EECs of dairy goats.

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“…In EC cells, BBR affects the distribution of the cell cycle and induces apoptotic conditions via activation of the mitochondrial-caspase pathway. Furthermore, since BBR engaged the PI3K/Akt pathway, it may be recommended as a functional ingredient for the prevention and treatment of endometrial cancers [ 117 ].…”

Section: Anticancer Perspectivesmentioning

confidence: 99%

Berberine as a Potential Anticancer Agent: A Comprehensive Review

et al. 2021

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Berberine (BBR), a potential bioactive agent, has remarkable health benefits. A substantial amount of research has been conducted to date to establish the anticancer potential of BBR. The present review consolidates salient information concerning the promising anticancer activity of this compound. The therapeutic efficacy of BBR has been reported in several studies regarding colon, breast, pancreatic, liver, oral, bone, cutaneous, prostate, intestine, and thyroid cancers. BBR prevents cancer cell proliferation by inducing apoptosis and controlling the cell cycle as well as autophagy. BBR also hinders tumor cell invasion and metastasis by down-regulating metastasis-related proteins. Moreover, BBR is also beneficial in the early stages of cancer development by lowering epithelial–mesenchymal transition protein expression. Despite its significance as a potentially promising drug candidate, there are currently no pure berberine preparations approved to treat specific ailments. Hence, this review highlights our current comprehensive knowledge of sources, extraction methods, pharmacokinetic, and pharmacodynamic profiles of berberine, as well as the proposed mechanisms of action associated with its anticancer potential. The information presented here will help provide a baseline for researchers, scientists, and drug developers regarding the use of berberine as a promising candidate in treating different types of cancers.

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Growth-suppressive effect of berberine on endometrial carcinoma cells: Role of mitochondrial and PI3K/Akt pathway

Cited by 12 publications

References 30 publications

miR‐26a promoted endometrial epithelium cells (EECs) proliferation and induced stromal cells (ESCs) apoptosis via the PTEN‐PI3K/AKT pathway in dairy goats

miR‐26a promoted endometrial epithelium cells (EECs) proliferation and induced stromal cells (ESCs) apoptosis via the PTEN‐PI3K/AKT pathway in dairy goats

miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression

Berberine as a Potential Anticancer Agent: A Comprehensive Review

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