Growth Stimulator for Bifidobacteria Produced by Propionibacterium freudenreichii and Several Intestinal Bacteria

Kaneko, Tsutomu; Mori, Hiroharu; Iwata, Megumi; Meguro, Sachiko

doi:10.3168/jds.s0022-0302(94)76965-4

Cited by 130 publications

(77 citation statements)

References 22 publications

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“…Together with other intestinal bacteria, P. freudenreichii has been observed to contain a bifidogenic factor in its cell-free extract [10]. This may have a positive influence on the composition of the intestinal microflora.…”

Section: Discussionmentioning

confidence: 99%

“…PAB are members of the normal intestinal microflora, though not as a major component, where they are thought to play a role in the digestion of protein [12], and contribute to the production of vitamin K [6]. Some health effects of PAB have been reported; stimulation of the immune system [8,11], stimulation of the growth of bifidobacteria [3,10] and reduction of mutagen-producing faecal enzyme activity [14,21]. The ability of PAB to suppress the growth of yeasts and fungi could also have considerable therapeutic use [23].…”

Section: Introductionmentioning

confidence: 99%

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In vitro adhesion of propionic acid bacteria to human intestinal mucus

Ouwehand

Suomalainen

Tölkkö

et al. 2002

Lait

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-Propionic acid bacteria (PAB) have widely been used as starter cultures. Their potential as probiotics has, however, received little attention. Adhesion to the intestinal mucosa is considered one of the main selection criteria for probiotic micro-organisms. Therefore, in the current study the adhesion of PAB to human intestinal mucus was investigated in an in vitro model. The tested PAB exhibited a low to moderate level of adhesion (0.4 to 4.5% of the applied bacteria). Because adhesion to mucus and bovine serum albumin were similar, it is likely that the adhesion to mucus is the result of non-specific interactions. The adhesion to mucus could be significantly enhanced by prior adhesion of existing probiotic strains. The PAB did not affect the adhesion of these probiotics. Adhesion of the moderately adhering strains was found to be close to maximum within 30 s showing that the adhesion happens almost instantaneously, while the adhesion of the low binding strains increased until 1 h of incubation. These results together with earlier observations on the properties of PAB indicate that selected PAB have good prerequisites for probiotic use. Propionibacterium / adhesion / intestinal mucus / probioticRésumé -Adhésion in vitro de bactéries propioniques au mucus intestinal humain. Les bacté-ries propioniques ont été largement utilisées comme levains. Leur potentiel comme probiotiques a cependant reçu peu d'attention. L'adhésion à la muqueuse intestinale est considérée comme l'un des principaux critères de sélection des microorganismes probiotiques. Ainsi, dans la présente étude, l'adhésion des bactéries propioniques au mucus intestinal humain a été examinée dans un modèle in vitro. Les bactéries propioniques testées ont montré un niveau d'adhésion bas à modéré (0,4 à 4,5 % des bactéries appliquées). Comme l'adhésion au mucus et à la sérum-albumine bovine était similaire, il est probable que l'adhésion au mucus est le résultat d'interactions non-spécifiques. L'adhésion au mucus pourrait être significativement accrue par l'adhésion préalable de souches probiotiques existantes. Les bactéries propioniques n'affectent pas l'adhésion de ces probiotiques. L'adhésion des

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro adhesion of propionic acid bacteria to human intestinal mucus

Ouwehand

Suomalainen

Tölkkö

et al. 2002

Lait

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“…freudenreichii could produce a strong growth stimulator for bifidobacteria (BGS) and that BGS activity was present even after heat treatment at 121°C for 15 min (20). Other species of propionibacteria such as P. jensenii and P. acidipropionici, which are usually detected in dairy products, also produced a substance like BGS (data are not shown).…”

Section: Propionibacteriamentioning

confidence: 99%

“…However, PQQ could not enhance the growth of bifidobacteria. In 1994, we found a strong and novel bifidogenic growth stimulator (BGS) produced by Propionibacterium freudenreichii, which is used extensively in the dairy industry as starter culture for the manufacture of Swiss-type cheese (20). The propionibacteria, especially P. freudenreichii, are essential for the development of the characteristic flavor and eye development in cheese making and are also important in other food industries, where they are responsible for the production of organic acids, biomass, vitamin B 12 and other specific metabolites (24,30).…”

Section: Introductionmentioning

confidence: 99%

A Novel Bifidogenic Growth Stimulator Produced by Propionibacterium freudenreichii

Kaneko

1999

Bioscience Microflora

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A novel bifidogenic growth stimulator (BGS) was present in the cell-free filtrate and in methanol extract fraction of the starter (Propionibacterium freudenreichii) cells for the manufacture of Swiss-type cheese. After purification of the BGS isolated from the lyophilized cells, the mass (217.037) was determined by high-resolution electron impact mass spectrometry (MS) and liquid chromatography-MS spectra. Various experimental analyses indicated that the chemical structure of the BGS was 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ). We examined the activity of the P. freudenreichii ET culture containing the BGS on a gut bacterial composition using an anaerobic continuous culture system and found that the BGS seems to enhance the selective utilization of oligosaccharides by bifidobacteria. Furthermore, a placebo-controlled study of the effects of BGS on fecal flora and stool frequency was carried out in healthy human subjects. A drink with the sterilized ET-3 culture was administered once a day for 7 days. Bifidobacterium percentage in the fecal flora and stool frequency were significantly increased by administration of the P. freudenreichii culture. The ACNQ exhibited growth stimulation of bifidobacteria at an extremely low concentration and enhanced the activities of NADH oxidase and NADH peroxidase in bifidobacteria. It was revealed that ACNQ works as a good electron acceptor of NAD(P)H diaphorase. The ACNQred was easily auto-oxidized and also acted as a better electron donor of NAD(P)H peroxidase. These ACNQ-mediated reactions seem to play roles in NAD(P)+-regeneration processes and seem to be responsible for the growth stimulation of bifidobacteria.

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“…Several substances known as bifidogenic factors are introduced to the gastrointestinal tract with food such as carbohydrates raffinose, melibiose, lactose and glycoproteins as well as N-acetyl-glucosamines present in women's milk. These compounds, to different extents, stimulate proliferation of the individual species of Bifidobacterium [4,7]. The propionic acid bacteria (PAB) also have a beneficial effect on the growth of Bifidobacterium.…”

Section: Introductionmentioning

confidence: 99%

Possibilities for stimulation of Bifidobacterium growth by propionibacteria

Warmińska-Radyko

Laniewska-Moroz

Babuchowski

2002

Lait

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-Twenty-seven propionic acid bacteria (PAB) strains were studied for their ability to produce metabolites, which stimulated the growth of six Bifidobacterium strains. In addition, the 27 investigated PAB strains were examined for their influence on the growth of four Gram(-) strains belonging to species Escherichia coli and Yersinia enterocolitica, their abilities to survive at pH 2.0, 2.5, 3.0 and in the presence of 4% bile salts as well as their sensitivity against 12 selected antibiotics. All PAB strains stimulated growth of from 3 to 6 of the examined Bifidobacterium strains and simultaneously produced antibacterial metabolites against Gram(-) rods. Fifteen PAB strains inhibited growth of the particular Bifidobacterium strains. Five PAB strains were resistant to acidity surviving for 2 h in pH 2.5 at population level 10 2 -10 6 cfu . mL -1 and 3 strains were resistant to bile salts. Only one PAB strain survived in both the acidic and the bile salt environments. All PAB strains were sensitive to a majority of the antibiotics used in the investigations. Bifidobacterium / Propionibacterium / Gram(-) rod / stimulation / inhibitionRésumé -Stimulation de la croissance de Bifidobacterium par les bactéries propioniques. Vingt sept souches de bactéries propioniques (PAB) ont été examinées pour leur aptitude à produire des métabolites stimulant la croissance de six souches de Bifidobacterium. Par ailleurs, ces 27 souches de PAB ont été examinées pour leur influence sur le développement de quatre souches Gram(-) des espèces Escherichia coli et Yersinia enterocolitica, pour leur capacité de survie dans un environnement à pH 2.0, 2.5, 3.0 et en présence de 4 % de sels biliaires ainsi que pour leur sensibilité à douze antibiotiques sélectionnés. Toutes les souches de PAB ont stimulé le développement de 3 à 6 des souches examinées de Bifidobacterium en produisant en même temps des métabolites antibactériens contre les bacilles Gram(-). Quinze souches de PAB ont eu un effet inhibiteur sur le développement des souches individuelles de Bifidobacterium. Cinq souches de PAB ont résisté à l'acidité et ont 113

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Growth Stimulator for Bifidobacteria Produced by Propionibacterium freudenreichii and Several Intestinal Bacteria

Cited by 130 publications

References 22 publications

In vitro adhesion of propionic acid bacteria to human intestinal mucus

In vitro adhesion of propionic acid bacteria to human intestinal mucus

A Novel Bifidogenic Growth Stimulator Produced by Propionibacterium freudenreichii

Possibilities for stimulation of Bifidobacterium growth by propionibacteria

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