Growth Stimulation of Bifidobacteria by a Mixed Culture with Vitamin B12-Producing Klebsiella pneumoniae IFO 13541

Noda, Hiroko; Fukuda, Shoko; Okamura, Tokumitsu; Osugi, Masahiro

doi:10.12938/bifidus1996.17.49

Cited by 1 publication

(1 citation statement)

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“…Specific nutritional requirements such as the presence of hemin, vitamin K and B vitamins are frequently linked with optimal growth of the intestinal bacteria during their cultivation. Noda et al (1998) observed the stimulatory effect of vitamin B 12 on the growth of Bifidobacteria , while vitamin K and hemin have been established as growth factors for Bacteroides melaninogenicus (Gibbons & MacDonnald, 1960). In addition, Bacteroides spp.…”

Section: Introductionmentioning

confidence: 99%

A protocol for the cultivation and monitoring of ileal gut microbiota surrogates

Akritidou

Smet

Akkermans

et al. 2022

Journal of Applied Microbiology

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Aims: This research aimed to develop and validate a cultivation and monitoring protocol that is suitable for a surrogate microbial community that accounts for the gut microbiota of the ileum of the small intestine.Methods and Results: Five bacterial species have been selected as representatives of the ileal gut microbiota and a general anaerobic medium (MS-BHI, as minimally supplemented brain heart infusion) has been constructed and validated against BCCM/LGM recommended and commercial media. Moreover, appropriate selective/differential media have been investigated for monitoring each ileal gut microbiota surrogate. Results showed that MS-BHI was highly efficient in displaying individual and collective behaviour of the ileal gut microbiota species, when compared with other types of media. Likewise, the selective/differential media managed to identify and describe the behaviour of their targeted species.Conclusions: MS-BHI renders a highly efficient, inexpensive and easy-to-prepare cultivation and enumeration alternative for the surrogate ileal microbiota species.Additionally, the selective/differential media can identify and quantify the bacteria of the surrogate ileal microbial community. Significance and Impact of Study:The selected gut microbiota species can represent an in vitro ileal community, forming the basis for future studies on small intestinal microbiota. MS-BHI and the proposed monitoring protocol can be used as a standard for gut microbiota studies that utilize conventional microbiological techniques.

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Section: Introductionmentioning

confidence: 99%