2001
DOI: 10.1105/tpc.010011
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Growth Stage–Based Phenotypic Analysis of Arabidopsis

Abstract: With the completion of the Arabidopsis genome sequencing project, the next major challenge is the large-scale determination of gene function. As a model organism for agricultural biotechnology, Arabidopsis presents the opportunity to provide key insights into the way that gene function can affect commercial crop production. In an attempt to aid in the rapid discovery of gene function, we have established a high throughput phenotypic analysis process based on a series of defined growth stages that serve both as… Show more

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Cited by 544 publications
(261 citation statements)
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“…Both plates and pots were kept at 4°C for 3 days to synchronize germination. The seedling samples were collected at stage 1.04 (Boyes et al, ), which was Day 9 at 27°C, Day 11 at 21°C, and Day 15 at 15°C (Figure S1, Panel A). The seedlings for the root samples were grown on vertical plates to ease the collection of the roots (Figure S1, Panel B).…”
Section: Methodsmentioning
confidence: 99%
“…Both plates and pots were kept at 4°C for 3 days to synchronize germination. The seedling samples were collected at stage 1.04 (Boyes et al, ), which was Day 9 at 27°C, Day 11 at 21°C, and Day 15 at 15°C (Figure S1, Panel A). The seedlings for the root samples were grown on vertical plates to ease the collection of the roots (Figure S1, Panel B).…”
Section: Methodsmentioning
confidence: 99%
“…A . thaliana rosettes were harvested at developmental growth stage 6.0,32 quenched by immediately freezing in the light at liquid nitrogen temperature, and stored at −80°C prior to extraction and HILIC/MS analysis.…”
Section: Methodsmentioning
confidence: 99%
“…thaliana liquid cultures were grown for 12-13 days on an orbital shaker platform (Barnstead, IL) at 150 rpm, in the ambient air (350 ppm CO 2 ) of a growth chamber (model M-40, EGC Inc., Chagrin Falls, OH), under constant white light intensity (80-100 E m −2 s −2 ) and temperature (23 • C). The seeds had been cleaned [43] and stored overnight at 4 • C prior to inoculation. The plant cultures grew in 500 mL shake flasks, each containing 200 mL B5 Gamborg media [44] with minimal organics (Sigma, St. Louis), 2% (w/v) sucrose and 0.1% agar and inoculated with ∼100 Columbia ecotype seeds.…”
Section: Acquisition Of Plant Samplesmentioning
confidence: 99%