Growth, sporulation and production of bioactive compounds by Bacillus subtilis R14

Carvalho, André Lacerda Ulysses de; Oliveira, Fábio Henrique Portella Corrêa de; Mariano, Rosa de Lima Ramos; Gouveia, Ester Ribeiro; Souto-Maior, Ana Maria

doi:10.1590/s1516-89132010000300020

Cited by 37 publications

(36 citation statements)

References 17 publications

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“…This finding was supported by the bioautography of the major anti-P. tracheiphila spot which appeared at 16 h and increased till 72 h. Our data corroborated several previous studies dealing with the kinetic of lipopeptide production by Bacillus species [15,32], the optimum of which correlated rather well with the sporulation process.…”

Section: Discussionsupporting

confidence: 91%

Production and identification of iturin A lipopeptide fromBacillus methyltrophicusTEB1 for control ofPhoma tracheiphila

Kalai-Grami

Karkouch

Naili

et al. 2016

J. Basic Microbiol.

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A lipopeptide-producing endophytic Bacillus methyltrophicus TEB1 strain exhibited potent antifungal activity against Phoma tracheiphila. Lipopeptide production started at the early growth phase plateaued after 36 h of culture where it reduced the mycelium growth by 80%. The crude lipopeptide extract harvested at the stationary phase efficiently inhibited the growth of P. tracheiphila mycelium and MIC values displaying 50 and 90% inhibition of conidia germination were around 47.5 and 100 μg ml(-1) , respectively. Increasing lipopeptide extract till 3 mg ml(-1) induced 10% swelling and 3% crumbling of P. tracheiphila conidia whereas 5 mg ml(-1) induced 40% swelling and 20% crumbling. Mass spectrometry analysis of the lipopeptide extract indicated that surfactin production took place from 12 to 20 h, iturin A from 16 to 72 h, and fengycin from 12 to 72 h and that the main active compound against P. tracheiphila was identified as C15 iturin A lipopeptide. Iturin A appeared as a potential biological control agent able to substitute the currently used chemical pesticides in agriculture.

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Section: Discussionsupporting

confidence: 91%

Production and identification of iturin A lipopeptide fromBacillus methyltrophicusTEB1 for control ofPhoma tracheiphila

Kalai-Grami

Karkouch

Naili

et al. 2016

J. Basic Microbiol.

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“…4B). Hence, sporulation is likely to cause optical density declines through mother cell lysis (Carvalho et al, 2010;Pompeo et al, 2016).…”

Section: Sporulating and Non-sporulating C Botulinum Cultures Show Dmentioning

confidence: 99%

Neurotoxin synthesis is positively regulated by the sporulation transcription factor Spo0A in Clostridium botulinum type E

Mascher

Mertaoja

Korkeala

et al. 2017

Environmental Microbiology

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Clostridium botulinum produces the most potent natural toxin, the botulinum neurotoxin (BoNT), probably to create anaerobiosis and nutrients by killing the host, and forms endospores that facilitate survival in harsh conditions and transmission. Peak BoNT production coincides with initiation of sporulation in C. botulinum cultures, which suggests common regulation. Here, we show that Spo0A, the master regulator of sporulation, positively regulates BoNT production. Insertional inactivation of spo0A in C. botulinum type E strain Beluga resulted in significantly reduced BoNT production and in abolished or highly reduced sporulation in relation to wild-type controls. Complementation with spo0A restored BoNT production and sporulation. Recombinant DNA-binding domain of Spo0A directly bound to a putative Spo0A-binding box (CTTCGAA) within the BoNT/E operon promoter, demonstrating direct regulation. Spo0A is the first neurotoxin regulator reported in C. botulinum type E. Unlike other C. botulinum strains that are terrestrial and employ the alternative sigma factor BotR in directing BoNT expression, C. botulinum type E strains are adapted to aquatic ecosystems, possess distinct epidemiology and lack BotR. Our results provide fundamental new knowledge on the genetic control of BoNT production and demonstrate common regulation of BoNT production and sporulation, providing a key intervention point for control.

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“…is also a key factor to optimize bioreactor operation. For B. subtilis, Carvalho et al (2010) report this phenomenon in aerobic cultivations after glucose depletion. With this scope, it is important to assess the consumption patterns of substrates, of cell growth and of metabolite generation for this microorganism.…”

Section: Introductionmentioning

confidence: 85%

Assessment of the metabolism of different strains of Bacillus megaterium

Suarez

Montano

Nucci

et al. 2012

Braz. arch. biol. technol.

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Bacillus megaterium is a promising host for expression of heterologous proteins. This paper reports the nutrient consumption patterns and production of metabolites for three different strains of B. megaterium, ATCC 14945, QMB 1551 and PV 361, which is QMB 1551 with seven constitutive plasmids deleted. 14 h cultivations in agitated flasks were run, for two different media: A (LB plus 10g/L glucose) and B (medium A, with the yeast extract replaced by tryptone). Strains PV361 and QMB 1551 showed higher maximum specific growth rates in medium B

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Growth, sporulation and production of bioactive compounds by Bacillus subtilis R14

Abstract: ABSTRACT

Cited by 37 publications

References 17 publications

Production and identification of iturin A lipopeptide fromBacillus methyltrophicusTEB1 for control ofPhoma tracheiphila

Production and identification of iturin A lipopeptide fromBacillus methyltrophicusTEB1 for control ofPhoma tracheiphila

Neurotoxin synthesis is positively regulated by the sporulation transcription factor Spo0A in Clostridium botulinum type E

Assessment of the metabolism of different strains of Bacillus megaterium

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