Growth retardation in human blastocysts increases the incidence of abnormal spindles and decreases implantation potential after vitrification

Hashimoto, Shu; Amo, A.; Hama, Satoko; Ito, Keijiro; Nakaoka, Yoshiharu; Morimoto, Yoshiharu

doi:10.1093/humrep/det059

Cited by 49 publications

(41 citation statements)

References 30 publications

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“…Our data parallels these reports; however, these present data also suggest that even those embryos that are diagnosed as euploid can develop to the blastocyst stage at varying rates, suggesting that other factors aside from chromosomes influence growth. For example, mitochondrial, spindle abnormalities, and specific gene expression have all been shown to influence embryo development (Hashimoto et al, 2013;Hsieh et al, 2004;Wood et al, 2007). The use of assisted hatching may confound our data, causing embryos to artificially blastulate prematurely.…”

Section: Discussionmentioning

confidence: 94%

“…Campbell et al (2013a;2013b) used a time-lapse culture system to show that aneuploid blastocysts were slower to blastulate compared with euploid blastocysts. The lower pregnancy and implantation rates associated with day 6 blastocysts could be attributed to spindle abnormalities, mitochondrial deficiencies or gene expression (Hashimoto et al, 2013;Hsieh et al, 2004;Shapiro et al, 2013;Wood et al, 2007). These deficiencies could affect the blastocysts' ability to implant and develop in-utero.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of aneuploidy, pregnancy and live birth rates between day 5 and day 6 blastocysts

Taylor

Patrick

Gitlin

et al. 2014

Reproductive BioMedicine Online

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Comprehensive chromosome screening is typically used for aneuploidy analysis of blastocysts. It is believed that either day of blastocyst development is acceptable. Euploidy rates and outcomes were examined between day 5 and day 6 blastocysts in two studies. First, euploidy rates of day 5 and day 6 blastocysts were examined on a per-embryo and per-patient basis. Second, outcomes were compared when only euploid day 5 or day 6 blastocysts were transferred in a cryopreserved embryo transfer cycle. In cycles (n = 70) that had blastocysts biopsied on both day 5 and day 6, day 5 blastocysts had a higher chance of being euploid than day 6 blastocysts (125/229 [54.6%]) and (77/180 [42.8%]), respectively (P = 0.0231). Similarly, euploid rates in blastocysts from patients (n = 193) with day 5 biopsy, day 6 biopsy, or both, were significantly higher in day 5 (235/421 [55.8%]) compared with day 6 (184/413 [44.6%]) blastocysts (P = 0.0014). In the second study, 50 women (36.1 ± 4.3 years) and 39 women (35.1 ± 3.8 years) with only euploid day 5 or euploid day 6 blastocysts transferred during a cryopreserved embryo transfer had similar cycle outcomes. Although underpowered, these data suggest that euploid day 6 blastocysts are as capable of positive outcomes as their euploid day 5 counterparts.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Comparison of aneuploidy, pregnancy and live birth rates between day 5 and day 6 blastocysts

Taylor

Patrick

Gitlin

et al. 2014

Reproductive BioMedicine Online

View full text Add to dashboard Cite

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“…The final inclusion was 13 studies that met the selection criteria for our meta‐analysis. Hence, 23 studies were included in the following meta‐analyses . The main characteristics and assessment for risk of bias of the included studies are presented in Table .…”

Section: Resultsmentioning

confidence: 99%

“…In line with this, we observed that transfer of euploid day 5 blastocyst resulted in higher implantation and live birth rate than the transfer of euploid day 6 blastocyst, while there was no significant difference between day 5 and day 6 blastocyst transfers for clinical pregnancy and ongoing pregnancy rates. Recent studies have found that intracellular factors, including spindle assembly checkpoints, abnormal spindles, microtubule kinetics, and mitochondrial function, can cause developmental delay in preimplantation embryo. The impact of intracellular factors on the euploid embryos may be a reason for the higher pregnancy rate of day 5 blastocyst transfer than day 6 blastocyst transfer …”

Section: Discussionmentioning

confidence: 99%

Pregnancy outcomes after day 5 versus day 6 blastocyst‐stage embryo transfer: A systematic review and meta‐analysis

Wang²,

Sun

et al. 2020

J of Obstet and Gynaecol

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Aim To compare the pregnancy outcomes after day 5 blastocyst‐stage embryo transfers (BET) versus day 6 BET following vitrified‐warmed cycle and to evaluate whether the number of embryos transferred and the chromosomal status of embryo influence effect estimates. Methods A literature search (PubMed, Embase and MEDLINE) up to January 2019 was conducted to identify studies where women with day 6 BET were compared to women with day 5 BET. Only studies published in English language, on peer‐reviewed journal were considered eligible. The following subgroup analyses were performed: (i) number of embryos transferred and (ii) chromosomal status of embryo. Results From a total of 1956 articles identified, 23 observational studies were included in the meta‐analysis. We observed that day 6 BET were associated with lower implantation rate (risk ratio, RR: 1.17, 95% confidence interval, CI: 1.10–1.24), clinical pregnancy rate (RR: 1.17, 95% CI: 1.10–1.24), ongoing pregnancy rate (RR: 1.15, 95% CI: 1.07–1.24) and live birth rate (RR: 1.22, 95% CI: 1.11–1.33) than day 5 BET following vitrified‐warmed cycle. The subgroup analysis found that the superiority of day 5 BET compared with day 6 BET is influenced by the number of embryos transferred and chromosomal status of embryos. Conclusion Current evidence shows that day 5 BET is superior to day 6 BET following vitrified‐warmed cycle in clinical practice. Due to the overall low quality of available evidence, more larger and well‐conducted studies are needed to compare the pregnancy outcomes between day 5 and day 6 BET before drawing a clear conclusion.

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“…The endometrium was prepared by administration of GnRH agonist (600 μg/day, Suprecur® nasal solution 0.15 %; Mochida Pharmaceutical; Tokyo, Japan) for 3 weeks followed by increasing doses of oral estradiol valerate (Progynova®; Bayer Schering Pharma; Zürich, Switzerland) from 1 to 4 mg for 2 weeks [30]. After ultrasonographical confirmation that the endometrium was thicker than 8 mm, chlormadinone acetate (Lutoral®; Shionogi & Co.; Osaka, Japan) was administered (6 mg/day).…”

Section: Preparation Of the Endometriummentioning

confidence: 99%

Neonatal outcomes after the implantation of human embryos vitrified using a closed-system device

Iwahata

Hashimoto

Inoue³

et al. 2015

J Assist Reprod Genet

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Purpose Closed vitrification poses a risk of adversely affecting embryo development, while it may minimize the risk of contamination. We assessed the effects of closed-system human embryo vitrification on fetal development after implantation, neonatal outcome, and clinical safety. Methods This was a retrospective cohort study conducted at a private fertility clinic. A total of 875 vitrified-warmed blastocysts that were single-transferred under hormone-replacement cycles between November 2011 and December 2013 were randomly divided into two groups (closed vitrification, n 313; open vitrification, n 562) after receiving the patients' consent forms. Developmental competence after implantation, including gestational age, birth weight, sex, Apgar score, and anomalies of newborns, after the transfer of blastocysts vitrified by closing vitrification was compared with that obtained in the case of open vitrification. Results There were no significant differences between the use of closed and open vitrification systems in embryo development after implantation, gestational age, birth weight, sex ratio, Apgar score, and congenital anomalies of newborns. Conclusion Human embryos can be vitrified using a closed vitrification system without impairment of neonatal development.

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Growth retardation in human blastocysts increases the incidence of abnormal spindles and decreases implantation potential after vitrification

Abstract: This study provided new insights into the possible implications of abnormalities in spindle formation in growth-retarded human blastocysts.

Cited by 49 publications

References 30 publications

Comparison of aneuploidy, pregnancy and live birth rates between day 5 and day 6 blastocysts

Comparison of aneuploidy, pregnancy and live birth rates between day 5 and day 6 blastocysts

Pregnancy outcomes after day 5 versus day 6 blastocyst‐stage embryo transfer: A systematic review and meta‐analysis

Neonatal outcomes after the implantation of human embryos vitrified using a closed-system device

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