Growth retardants stimulate guggulsterone production in the presence of fungal elicitor in fed-batch cultures of Commiphora wightii

Abstract

Guggulsterone, a hypolipidemic natural agent, is produced in resin canals of the plant Commiphora wightii. In this study, the stimulatory effects of growth retardants [ALAR (N,N-dimethylaminosuccinamic acid) and CCC (chlormequat chloride)] and fungal elicitor on guggulsterone accumulation in cell cultures of C. wightii are reported. CCC at 1 mg l -1 enhanced guggulsterone content (*123 lg l -1 ) when added on the fifth day after inoculation, while ALAR at 2.5 mg l -1 increased guggulsterone content (*116 lg l -1 ) when added on the tenth day. In a two-stage fed-batch process, combined treatment with fungal elicitor and growth retardant caused a significant increase (*353 lg l -1 ) in guggulsterone content in cell cultures after 17 days of growth. This represents an approximately fivefold increase over the guggulsterone contents in initial cultures of this plant.

Quantitative determination of guggulsterone in existing natural populations of Commiphora wightii (Arn.) Bhandari for identification of germplasm having higher guggulsterone content

“…Production of oleo-gum resin is a stress induced phenomenon. Both biotic and abiotic factors including ecological (geographical and seasonal), individual plant performance (genotypes and morphotypes), pathogens and elicitors (methyl jasmonate, ethrel and salicyclic acid) as well as cultivation practices affect the production of secondary metabolite guggulsterone in intact plants as well as in tissue culture (see Kulhari et al 2012;Suthar and Ramawat 2010). In the present investigation, a strong correlation was seen between average rainfall of the area and guggulsterone content; regions with lower rainfall exhibited higher amount of guggulsterone (Fig.…”

Effect of alar and CCC on induction and germination of bulbils in Curculigo orchioides grown in shake flask cultures

“…Alar is considered as one of the most systemic growth retardants and affects growth and metabolism by various ways (El-Sheibany et al 2007). Alar enhanced stilbenes content in root cultures of Cayratia trifolia (Arora et al 2009) and guggulsterone (Suthar and Ramawat 2010). CCC belongs to a group called onium compounds and blocks the cyclases, copalyl disphosphate sterase and ent-kaurene synthase involved in the early steps of GA metabolism (Rayirath et al 2009).…”

HPLC–ESI–MSn Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla

“…They also found that the mist interval related negatively to the average growth rate of the adventitious roots and positively to saponin and polysaccharide contents. In a fed-batch culture of Commiphora wightii cells, a combined treatment with a fungal elicitor and a growth retardant caused a significant increase in guggulsterone content after 17 days of growth [28]. This represented an approximately 5.0-fold increase over the guggulsterone contents in initial cultures of this plant.…”