In response to concerns that increment coring with an increment borer might contribute to the dieback of pine trees in Thailand, this research aimed to evaluate the effects of increment coring on the growth of Khasi pine (Pinus kesiya Royle ex Gordon) at Doi Khuntan National Park in northern Thailand. Increment coring is commonly used in dendrochronology, but its impact on tree growth needs to be better understood. This study involved the selection of pine trees of varying diameters, categorizing them into control (uncored) and experimental (cored) groups. Subsequently, data were collected bimonthly from September 2018 to April 2023, except for interruptions from February 2020 to December 2021 due to the COVID-19 pandemic. Tree diameters at breast height were measured, and image analysis was used to monitor the wound healing every two months. A repeated-measures ANOVA was used to compare the growth of cored and uncored groups and the wound healing rates among small-, medium-, and large-tree groups. The growth of cored and uncored Khasi pines within the same and different diameter classes showed no significant differences nor did the wound healing rates. The findings indicated that increment coring had an insignificant impact on the tree growth across all diameter classes, with wounds healing effectively within 14 months. These results support the continued and safe use of increment coring with an increment borer as a non-destructive method for collecting tree-ring and wood samples for climate research and for providing valuable insights into forest management practices.