Growth Response in Athymic Nude Mice to Transplanted Growth Hormone-Secreting Rat Pituitary Tumor Cells*

Shin, Seung-Il; Brown, André L.; Bancroft, F. Carter

doi:10.1210/endo-103-1-223

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…showed progressive growth ¡n nude mice with plasma rPRL lev els closely corresponding with tumor size. A rat GH-secreting tumor cell-line has also pre viously been reported to develop progres sively growing tumors after s.c. injection into nude mice with maintenance of rGH produc tion [19], From these data it seems that in contrast with malignant experimental pitu itary' tumors s.c. transplanted benign human pituitary adenomas decrease in size in nude mice, but still demonstrate secretory activi tyisolation of sufficient viable adenoma cells by trypsinization of the transplanted tis sue was unsuccessful, while the rat pituitary tumor yielded, besides dead cells, large num bers of viable cells. First of all, the small size of the tissue fragments may have hampered the isolation of a reasonable number of via ble cells.…”

Section: Discussionmentioning

confidence: 91%

Transplantation of Human Pituitary Adenomas into Nude Mice

Oosterom¹,

Verleun²,

Uitterlinden³

et al. 1983

Horm Res

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Invitro studies with human pituitary adenomas are limited by the small amount of tissue obtained, which may be contaminated by the surrounding normal pituitary tissue. In this study we investigated if the passage of adenoma tissue via thymusless nude mice could solve some of these problems. The secretory capacity of the transplanted human pituitary adenomas was demonstrated by the presence of hGH and/or hPRL in the plasma of the host mouse, while other human pituitary hormones (TSH, LH and FSH) were undetectable. The transplants, however, decreased in size with time although histologically viable adenoma tissue was recovered that resembled the original tumor. Upon trypsinization of the small tissue fragments no viable adenoma cells could be obtained. In contrast, an experimental malignant rat pituitary tumor grew steadily with time, resulting in high levels of rPRL in the nude mice recipients. Large numbers of viable tumor cells were recovered from these tumors. Thus, human pituitary adenomas transplanted in nude mice continue to release hormone(s), but the transplants decrease in size and cannot be used to isolate dispersed tumor cells for in vitro studies.

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Section: Discussionmentioning

confidence: 91%

Transplantation of Human Pituitary Adenomas into Nude Mice

Oosterom¹,

Verleun²,

Uitterlinden³

et al. 1983

Horm Res

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show abstract

“…GH3 cells, a cell line originally isolated from a rat pituitary tumor, form a discrete, well vascularized tumor when implanted s.c. in female Wistar-Furth rats (3,4,8,9). Tumors derived from GH3 cells secrete GH indistinguishable from native rat GH, as determined by its biological and immunological activity (9,10 All experiments were performed at 20-22TC. No differences were found in the Ca2+ currents of the myocytes isolated from control rats aged 3-5 mo, and they were treated as a single group.…”

Section: Methodsmentioning

confidence: 99%

Increase in T-type calcium current in atrial myocytes from adult rats with growth hormone-secreting tumors.

Best

1990

Proc. Natl. Acad. Sci. U.S.A.

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Growth hormone (GH) has pronounced effects on protein synthesis and cell growth in cardiac muscle from adult animals, although the mechanism of its action is not understood. Because Ca2+ has been implicated as a regulator of mitogenic processes in a number of tissues, we investigated whether GH affects the transmembrane movement of Ca2+ through voltageactivated channels of cardiac myocytes. Atrial and ventricular myocytes were isolated from adult rats with GH-secreting tumors and studied electrophysiologically by using patch-clamp techniques. Tumor-bearing rats re-enter an active growth phase and double their body weight over age-matched controls 8 weeks after introduction of the tumor. Atrial myocytes from tumorbearing animals showed a 3-fold increase in the density ofT-type Ca2+ current compared with cells from control animals, although the voltage dependency of activation and inactivation of T-type current was not altered. The increase in T-current density of atrial myocytes preceded by at least a week any measurable change in heart weight, body weight, or myocyte size. L-type Ca2+ currents in atrial and ventricular cells were not affected. The results suggest that a tumor-derived growth factor, most likely GH, can cause a specific enhancement ofT-type Ca2+ current in atrial myocytes.

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ILMBIS: A One-Year-Old Experiment Co-Operative Interferon Research

Krim¹

1979

Antiviral Mechanisms in the Control of Neoplasia

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Growth Response in Athymic Nude Mice to Transplanted Growth Hormone-Secreting Rat Pituitary Tumor Cells*

Cited by 5 publications

References 16 publications

Transplantation of Human Pituitary Adenomas into Nude Mice

Transplantation of Human Pituitary Adenomas into Nude Mice

Increase in T-type calcium current in atrial myocytes from adult rats with growth hormone-secreting tumors.

ILMBIS: A One-Year-Old Experiment Co-Operative Interferon Research

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