Growth requirements of filamentous bacteria isolated from bulking and scumming sludge

Kämpfer, Peter; Weltin, D.; Hoffmeister, Detlef; Dott, Wolfgang

doi:10.1016/0043-1354(94)00275-c

Cited by 30 publications

(18 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A Proteiniphilum-like bacterium from the phylum Bacteroidetes dominated the microbial community in both reactors. Filamentous members of the Bacteroidetes phylum have been isolated from many environments [36][37][38][39]. However, little is known about the involvement of these microorganisms in bulking or foaming up until now [40], and the morphology of species from the genus Proteiniphilum was described as rod-shaped [41].…”

Section: Process Disruption-associated Differences In the Microbial Cmentioning

confidence: 99%

Floating layer formation, foaming, and microbial community structure change in full-scale biogas plant due to disruption of mixing and substrate overloading

et al. 2013

View full text Add to dashboard Cite

show abstract

Section: Process Disruption-associated Differences In the Microbial Cmentioning

confidence: 99%

Floating layer formation, foaming, and microbial community structure change in full-scale biogas plant due to disruption of mixing and substrate overloading

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Isolation can be facilitated by using a centrifugation or vortex mix pretreatment [20]. The organisms grow on I medium [22] and GMBN medium [30, 49] at 25°C. A detailed nutritional study has shown that few carbon sources are utilized [30, 49].…”

Section: Taxonomy Of Filamentous Bacteria Causing Foaming and Bulkinmentioning

confidence: 99%

Detection and cultivation of filamentous bacteria from activated sludge

Kämpfer

2006

FEMS Microbiology Ecology

Self Cite

View full text Add to dashboard Cite

Different applications of the activated sludge treatment process are employed globally for the biological treatment of wastewater. Since the activated sludge process was first developed, different kinds of sedimentation problems, in particular bulking sludge and foaming, have been observed and it is widely accepted that extensive, uncontrolled growth of filamentous bacteria is the main cause of these difficulties. Since the pioneering work of Eikelboom and coworkers who characterized and described seven groups of 26 morphological types of filamentous organisms several attempts have been made to develop new methods for isolation, cultivation and identification of the isolates. Improvement has also been sought in the methods for direct detection, to allow a better understanding of the complex phenomena of sludge bulking and foaming. However, although knowledge of the phylogeny of filamentous organisms has increased recently, only a few attempts have been made to achieve a better understanding of their growth characteristics. The aim of this paper is to give an overview of detection and cultivation methods with special emphasis on the comparison of classical approaches with the modern molecular biological methods, including in situ detection and identification of filamentous bacteria.

show abstract

“…Isolation of filamentous bacteria is an essential requirement for the investigation of biochemical and metabolic kinetics. However, systematic approaches to the nutritional requirements of different filamentous organisms are limited due to the difficulty of isolating them (Ka¨mpfer et al 1995;Ka¨mpfer 1997).…”

Section: Introductionmentioning

confidence: 99%

‘Morphological shifts’ in filamentous bacteria isolated from activated sludge processes

Ramothokang

Naidoo

Bux

2006

World J Microbiol Biotechnol

View full text Add to dashboard Cite

This study aimed at isolating filamentous bacteria from full-scale activated sludge processes and studying them in pure culture. Three cultures were isolated using conventional microbiological techniques. The isolates were positively identified as Gordonia amarae, Thiothrix nivea and Type 1863/Acinetobacter spp., using fluorescent in situ hybridization (FISH) with 16S rRNA-targeted oligonucleotide probes. However, a 'morphological shift' from filamentous to single-cell form was observed in pure culture. The application of fluorescent in situ hybridization (FISH) showed filamentous bacteria to be much more diverse in their ability to adapt to their changing enviroments. Pure culture studies of filamentous bacteria form the basis for application in full-scale activated sludge plants. It therefore remains important that the taxonomic status of filamentous bacteria be determined.

show abstract

Growth requirements of filamentous bacteria isolated from bulking and scumming sludge

Cited by 30 publications

References 11 publications

Floating layer formation, foaming, and microbial community structure change in full-scale biogas plant due to disruption of mixing and substrate overloading

Floating layer formation, foaming, and microbial community structure change in full-scale biogas plant due to disruption of mixing and substrate overloading

Detection and cultivation of filamentous bacteria from activated sludge

‘Morphological shifts’ in filamentous bacteria isolated from activated sludge processes

Contact Info

Product

Resources

About