Growth regulator pretreatment improves somatic embryogenesis from leaves of squash (C ucurbita pepo l.) and melon ( Cucumis melo l.)

Abstract

Leaf explants of squash (Cucurbita pepo L.) and melon (Cucumis melo L.) were pretreated initially with 113.1, 226.2 or 452.4 µM 2,4-dichlorophenoxyacetic acid (2,4-D), 46.5, 93 or 186 µM kinetin or a combination of both at the above concentrations, for 6, 24 or 48 h. After pretreatment, explants were transferred to an agar-solidified medium that was not supplemented with growth regulators or to a species-specific standard induction medium. Control explants from each species were incubated directly on the species-specific standard induction medium. Initial pretreatment of squash explants with 186 µM kinetin and of melon explants with 226.2 µM 2,4-D for 48 h significantly promoted the formation of somatic embryos which developed further to the torpedo-shape stage and germinated. Under these conditions at least four plants can be regenerated per square centimeter of explant surface, thus achieving an increase over non-pretreated cultures of 143% and 130% for squash and melon, respectively.Growth regulator pretreatment improves somatic embryogenesis from leaves of squash (Cucurbita pepo l.) and melon (Cucumis melo l.)

Establishment of Shoot - tip Regeneration System of Watermelon

“…In 141 Cucurbitaceae, 6-BA plays an important role in explant tissue culture induction of 142 adventitious bud differentiation. This is also true in cucumber and pumpkin plant 143 tissue regeneration which was confirmed by experiments (Kintzios, et al, 2002). In Moreover, the 6-BA concentration they used was higher than that in this experiment.…”

Optimization of regeneration using differential growth regulators in indica rice cultivars

“…This may be because cytokinin causes development of single pole and formation of meristematic dome from which the shoot primordium develops. The role of cytokinins on somatic embryogenesis has been explained by enhanced cell division of pre-embryogenically determined cells (Kintzois et al 2002). In addition to these reports, kinetin increases proliferation and regeneration of callus by affecting mitosis, cytokinesis, total protein synthesis, lignin biosynthesis, vascular differentiation and differentiation of mature chloroplast from protoplastids (Wan et al 1988).…”

Production of transgenic periclinal chimeras in pumpkin – a tool for revealing cell fates of L1 meristem

“…Like most plants, pumpkins have two regenerative pathways: somatic embryogenesis and organogenesis. There have been many studies of somatic embryogenesis in C. pepo D. However, somatic embryos are difficult to induce and have low efficiency (Gonsalves et al 1995;Kintzios et al 2002;Urbanek et al 2004;Leljak-Levanic et al 2016). Compared with somatic embryo induction, organ regeneration occurs at a relatively high frequency and over a short experimental period.…”