Growth rates of Actinomyces viscosus and Streptococcus mutans during early colonization of tooth surfaces in gnotobiotic rats

Beckers, H. J.; Hoeven, J.S. van der

doi:10.1128/iai.35.2.583-587.1982

Cited by 64 publications

(18 citation statements)

References 23 publications

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“…Less interest has been shown in the growth of the oral microflora and the possible role of saliva as a substrate, yet the rapid accumulation of bacteria on tooth surfaces (21), even in the absence of diet (1), indicates that a continuous supply of nutrients supports the growth of the oral microflora. Possible sources of nutrients for bacteria in the mouth include the host diet, saliva, crevicular fluid, and desquamated epithelial cells.…”

mentioning

confidence: 99%

Growth of oral Streptococcus species and Actinomyces viscosus in human saliva

Jong¹,

Hoeven²,

Os³

et al. 1984

Appl Environ Microbiol

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Microorganisms in dental plaque live in constant association with saliva. The role of saliva in the adherence of bacteria to the teeth and the antibacterial properties of saliva have been well investigated; less interest has been shown in the possible role of saliva as a substrate for oral microorganisms. In this study it was shown that saliva can serve as a growth medium for oral Streptococcus spp. and Actionomyces viscosus. The cell production of these organisms on saliva was carbohydrate limited. The doubling times for growth on glucose-supplemented saliva (4 to 5 mmol/liter) ranged from 1.6 to 4.0 h. The availability of carbohydrate sources for the oral microflora is discussed in relation to microbial growth in the oral cavity.

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mentioning

confidence: 99%

Growth of oral Streptococcus species and Actinomyces viscosus in human saliva

Jong¹,

Hoeven²,

Os³

et al. 1984

Appl Environ Microbiol

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“…No evidence is available for decreased attachment or increased release of cells. The initial adherence of S. mutans in conventional rats is even higher (70 CFU/106 inoculated cells, 2 h after inoculation) than its adherence in gnotobiotic rats fed a sucrose diet (3 CFU/106 inoculated cells) or starved (16 CFU/106 inoculated cells) (1). Otherwise, there is considerable evidence that sucrose promotes the accumulation of S. mutans on the tooth surface (4, 10, 21), so increased release of cells in the presence of sucrose is unlikely.…”

Section: Resultsmentioning

confidence: 93%

“…During early colonization these latter processes are likely to be of little importance and the population increase will be mainly determined by cell division. At a later stage, during transition to or in steady-state situation, the processes of cell release, cell death, and settling of cells will be of more importance, and calculated doubling times, based on the determination of colony-forming units (CFU) of the population, reflect only the net result of all the involved processes (1).…”

mentioning

confidence: 99%

Effect of microbial interaction on the colonization rate of Actinomyces viscosus or Streptococcus mutans in the dental plaque of rats

Beckers¹,

Hoeven²

1982

Infect Immun

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The resident oral microflora of conventional Osborne-Mendel rats was challenged with Actinomyces viscosus or Streptococcus mutans strains. The adherence of the inoculated organism to the tooth surface and the subsequent growth were studied by means of viable counts determination. The initial growth rate of S. mutans in conventional rats was lower than in mono-associated gnotobiotic rats (doubling time, td = 5 h versus td = 1.1 h). The delayed start of growth and the low initial growth rate indicated that a competitive interaction between S. mutans and the resident microflora occurred. The initial growth rate of A. viscosus in conventional rats (td = 3.1 h) was approximately the same as that in gnotobiotic rats (td = 2.8 h). The start of growth of A. viscosus was only slightly delayed compared with the start in gnotobiotic rats. These results suggest a neutralistic relationship between A. viscosus and the resident microflora. A. viscosus reached a stationary level about 7 days after inoculation, whereas the S. mutans strains did not reach stationary levels until 2 weeks after inoculation.

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“…The molars were ground in a sterile mortar containing 0.~ ml of 0.85% (w/v) NaCI. After the mortar was rinsed with an additional 0.5 ml of 0.85% NaC1, the suspension was transferred to a sterile tube and dispersed for 30 s with a Kontes E/MC sonifier [18]. Suitable dilutions of the samples were plated onto TSY agar.…”

Section: Samplesmentioning

confidence: 99%

Effect of utilization of substrates on the composition of dental plaque

Hoeven

Jong

Rogers

1985

FEMS Microbiology Letters

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The microbiota in the mouth is subjected to substrate limitations. In this study we have evaluated the role of competition for carbon and energy substrates on the proportions of 2 microbial species in a simplified plaque ecosystem in gnotobiotic rats. Germ‐free rats were inoculated with a combination of Streptococcus sanguis and Streptococcus mutans, or with a combination of Streptococcus milleri and S. mutans. The available carbon and energy sources were varied through the host's diet. 3 Experimental diets were tested: (i) a basal diet low in soluble carbohydrates; (ii) an arginine‐supplemented diet; (iii) a sucrose‐supplemented diet. Arginine is used for growth by S. sanguis and S. milleri, but not by S. mutans. Sucrose is rapidly fermented by all 3 species. The total number of viable organisms on the dentition increased when arginine or sucrose were supplied in the diet. With the arginine‐supplemented diet, S. sanguis and S. milleri increased while S. mutans decreased. With the sucrose‐supplemented diet, S. mutans increased while S. sanguis and S. milleri decreased. These results were explained by assuming that the organism with the highest growth rate on the supplementary substrate competes most favourably. Changes in the environmental pH, due to breakdown of sucrose and arginine, might also have affected the competition between the streptococci. In addition, production of extracellular glucans from sucrose could be a competitive advantage for S. mutans.

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Growth rates of Actinomyces viscosus and Streptococcus mutans during early colonization of tooth surfaces in gnotobiotic rats

Cited by 64 publications

References 23 publications

Growth of oral Streptococcus species and Actinomyces viscosus in human saliva

Growth of oral Streptococcus species and Actinomyces viscosus in human saliva

Effect of microbial interaction on the colonization rate of Actinomyces viscosus or Streptococcus mutans in the dental plaque of rats

Effect of utilization of substrates on the composition of dental plaque

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