Growth promotion in Arabidopsis thaliana by bacterial cyclodipeptides involves the TOR/S6K pathway activation

González-López, Omar; Palacios-Nava, Brenda Berenice; Peña-Uribe, César Arturo; Campos-Garcı́a, Jesús; López‐Bucio, José; García-Pineda, Ernesto

doi:10.1016/j.jplph.2020.153343

Cited by 10 publications

(5 citation statements)

References 29 publications

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“…The three control isolates from human origin used in this study, PAO1, ATCC10145 and ATCC9027, had the plant-beneficial traits comparable with the agricultural P. aeruginosa (Figures 5-9). Previous studies have shown that PAO1 improves seed germination and promotes root growth in Arabidopsis (Chahtane et al, 2018;González-López et al, 2021). This finding further argues for the evolutionary relatedness of the plant-and human-associated strains.…”

Section: P Aeruginosa Can Promote Plant Growthmentioning

confidence: 60%

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Rhizospheric and endophyticPseudomonas aeruginosain edible vegetable plants share molecular and metabolic traits with clinical isolates

Ambreetha

Marimuthu

Mathee

et al. 2022

Journal of Applied Microbiology

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Aim: Pseudomonas aeruginosa, a leading opportunistic pathogen causing hospitalacquired infections, is also commonly found in agricultural settings. However, there are minimal attempts to examine the molecular and functional attributes shared by agricultural and clinical strains of P. aeruginosa. This study investigates the presence of P. aeruginosa in edible vegetable plants (including salad vegetables) and analyses the evolutionary and metabolic relatedness of the agricultural and clinical strains. Methods and Results:Eighteen rhizospheric and endophytic P. aeruginosa strains were isolated from cucumber, tomato, eggplant, and chili directly from the farms.The identity of these strains was confirmed using biochemical and molecular assays. The genetic and metabolic traits of these plant-associated P. aeruginosa isolates were compared with clinical strains. DNA fingerprinting and 16S rDNA-based phylogenetic analyses revealed that the plant-and human-associated strains are evolutionarily related. Both agricultural and clinical isolates possessed plant-beneficial properties, including mineral solubilization to release essential nutrients (phosphorous, potassium, and zinc), ammonification, and the ability to release extracellular pyocyanin, siderophore, and indole-3 acetic acid. Conclusion: These findings suggest that rhizospheric and endophytic P. aeruginosa strains are genetically and functionally analogous to the clinical isolates. In addition, the genotypic and phenotypic traits do not correlate with plant sources or ecosystems. Significance and Impact of the Study:This study reconfirms that edible plants are the potential source for human and animal transmission of P. aeruginosa.

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Section: P Aeruginosa Can Promote Plant Growthmentioning

confidence: 60%

“…aeruginosa (Figures 5–9). Previous studies have shown that PAO1 improves seed germination and promotes root growth in Arabidopsis (Chahtane et al, 2018; González‐López et al, 2021). This finding further argues for the evolutionary relatedness of the plant‐ and human‐associated strains.…”

Section: Discussionmentioning

confidence: 96%

Rhizospheric and endophyticPseudomonas aeruginosain edible vegetable plants share molecular and metabolic traits with clinical isolates

Ambreetha

Marimuthu

Mathee

et al. 2022

Journal of Applied Microbiology

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“…Uptake of the dipeptide Gly-Sar can mediate mTOR activity in leukemia stem cells ( Naka et al, 2015 ). Interestingly, several bacterial Pro-containing cyclodipeptides were proven to activate the TOR signaling pathway increasing growth in plants ( Corona-Sánchez et al, 2019 ; González-López et al, 2021 ), including cyclodipeptides versions of Pro-Phe, which oscillated only in raptor1b ( Supplementary Figures 2 , 3 ) and Pro-Val, whose level was increased in the mutant compared to Col-0 under LD ( Figure 3 ; Supplementary Figure 4 ). Among the 124 proteins identified as putative Pro-Gln interactors, we retrieved four related to the TOR pathway, besides several enzymes involved in C metabolism, such as glycolysis, gluconeogenesis, TCA cycle, and PPP ( Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Interestingly, the feeding of leukemia stem cells with specific dipeptides affects mTOR activity and nutrient signaling ( Naka et al, 2015 ). Moreover, cyclodipeptides released by bacteria activated the TOR pathway and increased growth in plants ( Corona-Sánchez et al, 2019 ; González-López et al, 2021 ). Recent system-wide characterization of the protein-protein-metabolite complexes in the model plant Arabidopsis thaliana provided evidence supporting the role of proteinogenic dipeptides in the regulation of enzymatic activities and C flux distribution ( Veyel et al, 2017 , 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Proteogenic Dipeptides Are Characterized by Diel Fluctuations and Target of Rapamycin Complex-Signaling Dependency in the Model Plant Arabidopsis thaliana

et al. 2021

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As autotrophic organisms, plants capture light energy to convert carbon dioxide into ATP, nicotinamide adenine dinucleotide phosphate (NADPH), and sugars, which are essential for the biosynthesis of building blocks, storage, and growth. At night, metabolism and growth can be sustained by mobilizing carbon (C) reserves. In response to changing environmental conditions, such as light-dark cycles, the small-molecule regulation of enzymatic activities is critical for reprogramming cellular metabolism. We have recently demonstrated that proteogenic dipeptides, protein degradation products, act as metabolic switches at the interface of proteostasis and central metabolism in both plants and yeast. Dipeptides accumulate in response to the environmental changes and act via direct binding and regulation of critical enzymatic activities, enabling C flux distribution. Here, we provide evidence pointing to the involvement of dipeptides in the metabolic rewiring characteristics for the day-night cycle in plants. Specifically, we measured the abundance of 13 amino acids and 179 dipeptides over short- (SD) and long-day (LD) diel cycles, each with different light intensities. Of the measured dipeptides, 38 and eight were characterized by day-night oscillation in SD and LD, respectively, reaching maximum accumulation at the end of the day and then gradually falling in the night. Not only the number of dipeptides, but also the amplitude of the oscillation was higher in SD compared with LD conditions. Notably, rhythmic dipeptides were enriched in the glucogenic amino acids that can be converted into glucose. Considering the known role of Target of Rapamycin (TOR) signaling in regulating both autophagy and metabolism, we subsequently investigated whether diurnal fluctuations of dipeptides levels are dependent on the TOR Complex (TORC). The Raptor1b mutant (raptor1b), known for the substantial reduction of TOR kinase activity, was characterized by the augmented accumulation of dipeptides, which is especially pronounced under LD conditions. We were particularly intrigued by the group of 16 dipeptides, which, based on their oscillation under SD conditions and accumulation in raptor1b, can be associated with limited C availability or photoperiod. By mining existing protein-metabolite interaction data, we delineated putative protein interactors for a representative dipeptide Pro-Gln. The obtained list included enzymes of C and amino acid metabolism, which are also linked to the TORC-mediated metabolic network. Based on the obtained results, we speculate that the diurnal accumulation of dipeptides contributes to its metabolic adaptation in response to changes in C availability. We hypothesize that dipeptides would act as alternative respiratory substrates and by directly modulating the activity of the focal enzymes.

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Pseudomonas aeruginosa LasI-dependent plant growth promotion requires the host nitrate transceptor AtNRT1.1/CHL1 and the nitrate reductases NIA1 and NIA2

López-Bucio,

Ortiz-Castro,

Magaña-Dueñas

et al. 2023

Planta

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Growth promotion in Arabidopsis thaliana by bacterial cyclodipeptides involves the TOR/S6K pathway activation

Cited by 10 publications

References 29 publications

Rhizospheric and endophyticPseudomonas aeruginosain edible vegetable plants share molecular and metabolic traits with clinical isolates

Rhizospheric and endophyticPseudomonas aeruginosain edible vegetable plants share molecular and metabolic traits with clinical isolates

Proteogenic Dipeptides Are Characterized by Diel Fluctuations and Target of Rapamycin Complex-Signaling Dependency in the Model Plant Arabidopsis thaliana

Pseudomonas aeruginosa LasI-dependent plant growth promotion requires the host nitrate transceptor AtNRT1.1/CHL1 and the nitrate reductases NIA1 and NIA2

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