Growth-promoting bacteria double eicosapentaenoic acid yield in microalgae

Liu, Bingli; Eltanahy, Eladl; Liu, Hongwei; Chua, Elvis T.; Thomas‐Hall, Skye R.; Wass, Taylor J.; Pan, Kehou; Schenk, Peer M.

doi:10.1016/j.biortech.2020.123916

Cited by 47 publications

(19 citation statements)

References 45 publications

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“…Different incubation groups (modified f/2 medium without microalgae, microalgae, 3.0 μm filtered solution, and 0.2 μm filtered solution) were set to explore the potential effects of culture medium, microalgae, associated bacteria, and extracellular substances on 199 Hg 2+ reduction, respectively. The bacteria associated with algae can be divided into algae-attached and free-living bacteria. − Microalgae culture was filtered through a 3.0 μm membrane filter to obtain the microalgae with attached bacteria in the retentate. The filtrate was further passed through a 0.2 μm membrane filter to retain the free-living bacteria.…”

Section: Methodsmentioning

confidence: 99%

Dark Reduction of Mercury by Microalgae-Associated Aerobic Bacteria in Marine Environments

Zhang

Liu

et al. 2021

Environ. Sci. Technol.

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Redox transformation of mercury (Hg) is critical for Hg exchange at the air−sea interface and it can also affect the methylation of Hg in marine environments. However, the contributions of microalgae and aerobic bacteria in oxic seawater to Hg 2+ reduction are largely unknown. Here, we studied the reduction of Hg 2+ mediated by microalgae and aerobic bacteria in surface marine water and microalgae cultures under dark and sunlight conditions. The comparable reduction rates of Hg 2+ with and without light suggest that dark reduction by biological processes is as important as photochemical reduction in the tested surface marine water and microalgae cultures. The contributions of microalgae, associated free-living aerobic bacteria, and extracellular substances to dark reduction were distinguished and quantified in 7 model microalgae cultures, demonstrating that the associated aerobic bacteria are directly involved in dark Hg 2+ reduction. The aerobic bacteria in the microalgae cultures were isolated and a rapid dark reduction of Hg 2+ followed by a decrease of Hg 0 was observed. The reduction of Hg 2+ and re-oxidation of Hg 0 were demonstrated in aerobic bacteria Alteromonas spp. using double isotope tracing ( 199 Hg 2+ and 201 Hg 0 ). These findings highlight the importance of algae-associated aerobic bacteria in Hg transformation in oxic marine water.

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Section: Methodsmentioning

confidence: 99%

Dark Reduction of Mercury by Microalgae-Associated Aerobic Bacteria in Marine Environments

Zhang

Liu

et al. 2021

Environ. Sci. Technol.

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“…The EPA content in BHT-treated cultures also increased to 0.233 g g −1 algal biomass. The co-cultivation of N. oceanica with microalgae-associated probiotic bacteria led to a 2.5-fold increase in the EPA content, along with a 1.56-fold increase in biomass (Liu et al, 2020).…”

Section: Microalgal Strain Improvementmentioning

confidence: 99%

Sustainable production of eicosapentaenoic acid-rich oil from microalgae: Towards an algal biorefinery

Sivakumar

Sachin

Priyadarshini

et al. 2022

Journal of Applied Microbiology

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Utilization of sustainable natural resources such as microalgae has been considered for the production of biofuels, aquaculture feed, high‐value bioactives such as omega‐3 fatty acids, carotenoids, etc. Eicosapentaenoic acid (EPA) is an omega‐3 fatty acid present in fish oil, which is of physiological importance to both humans and fish. Marine microalgae are sustainable sources of lipid rich in EPA, and different species have been explored for the production of EPA as a single product. There has been a rising interest in the concept of a multi‐product biorefinery, focusing on the maximum valorization of the algal biomass. Targeting one or more value‐added compounds in a biorefinery scenario can improve the commercial viability of low‐value products such as triglycerides for biofuel. This approach has been viewed by technologists and experts as a sustainable and economically feasible possibility for the large‐scale production of microalgae for its potential applications in biodiesel and jet fuel production, nutraceuticals, animal and aquaculture feeds, etc. In this review paper, we describe the recent developments in the production of high‐value EPA‐rich oil from microalgae, emphasizing the upstream and downstream bioprocess techniques, and the advantages of considering an EPA‐rich oil‐based biorefinery.

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“…After cultivation, biomass is usually harvested by various types of ultrafiltration steps, centrifugation, and electrocoagulation, which are not economically advantageous [108,139,140,142,143,146]. Pelletization and flocculation are usually used as low-cost downstream processing methods that reduce the cost of separating microalgae from the media.…”

Section: Downstream Processing and Analysis Of Algae And Bacteria Co-...mentioning

confidence: 99%

Biotechnological Enhancement of Probiotics through Co-Cultivation with Algae: Future or a Trend?

et al. 2022

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The diversity of algal species is a rich source of many different bioactive metabolites. The compounds extracted from algal biomass have various beneficial effects on health. Recently, co-culture systems between microalgae and bacteria have emerged as an interesting solution that can reduce the high contamination risk associated with axenic cultures and, consequently, increase biomass yield and synthesis of active compounds. Probiotic microorganisms also have numerous positive effects on various aspects of health and represent potent co-culture partners. Most studies consider algae as prebiotics that serve as enhancers of probiotics performance. However, the extreme diversity of algal organisms and their ability to produce a plethora of metabolites are leading to new experimental designs in which these organisms are cultivated together to derive maximum benefit from their synergistic interactions. The future success of these studies depends on the precise experimental design of these complex systems. In the last decade, the development of high-throughput approaches has enabled a deeper understanding of global changes in response to interspecies interactions. Several studies have shown that the addition of algae, along with probiotics, can influence the microbiota, and improve gut health and overall yield in fish, shrimp, and mussels aquaculture. In the future, such findings can be further explored and implemented for use as dietary supplements for humans.

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Growth-promoting bacteria double eicosapentaenoic acid yield in microalgae

Cited by 47 publications

References 45 publications

Dark Reduction of Mercury by Microalgae-Associated Aerobic Bacteria in Marine Environments

Dark Reduction of Mercury by Microalgae-Associated Aerobic Bacteria in Marine Environments

Sustainable production of eicosapentaenoic acid-rich oil from microalgae: Towards an algal biorefinery

Biotechnological Enhancement of Probiotics through Co-Cultivation with Algae: Future or a Trend?

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