Growth profiling, kinetics and substrate utilization of low-cost dairy waste for production of β-cryptoxanthin by
            <i>Kocuria marina</i>
            DAGII

Mitra, Ruchira; Dutta, Debjani

doi:10.1098/rsos.172318

Cited by 17 publications

(11 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A logistic kinetic model that stimulates growth was suggested by Mitra and Dutta 47 and was adopted to determine the biomass growth ( R. oryzae ) in this study. According to Ajala et al ., 48 the model is characterized by the biomass growth inhibition, independent of substrate and product, which is a common phenomenon encountered in batch culture.…”

Section: Methodsmentioning

confidence: 99%

“…The equation shows that the rate of product formation is directly and linearly proportional to growth rate and instantaneous biomass concentration 47 . However, integrating Eqn (9) using the appropriate boundary conditions is represented as Eqn (10):

\int_{0}^{P} d P = \propto \int_{0 - Δ t}^{t - Δ t} \frac{d X}{d t} d t + β true \int_{0 - Δ t}^{t - Δ t} X d t

According to Mitra and Dutta, 47 the term ‘Δ t ’ represented delay in the LA production with respect to biomass growth.…”

Section: Methodsmentioning

confidence: 99%

\int_{0}^{P} d P = \propto \int_{0 - Δ t}^{t - Δ t} \frac{d X}{d t} d t + β true \int_{0 - Δ t}^{t - Δ t} X d t

According to Mitra and Dutta, 47 the term ‘Δ t ’ represented delay in the LA production with respect to biomass growth. Substituting Eqn (5) into Eqn (10) gives a non‐linear relationship between product (P) and time (t) as shown in Eqn (11):

\begin{matrix} P = α X_{o} [\frac{e^{μ_{\max} t}}{1 - (\frac{X_{o}}{X_{m}}) (1 - e^{μ_{\max} t})}] \\ + β \frac{X_{m}}{μ_{\max}} \ln [1 - \frac{X_{o}}{X_{m}} (1 - e^{μ_{\max} t})] \end{matrix}

The plot of P against t in a non‐linear curve was used to evaluate the parameters of α and β .…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Lactic acid production: Utilization of yam peel hydrolysate as a substrate using Rhizopus orysae in kinetic studies

Ajala

Onoriemu

et al. 2021

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

The prospect of utilizing yam peel hydrolysate (YPH) as a carbon source for lactic acid (LA) production using Rhizopus orysae was investigated in batch surface and submerged fermentation processes. The kinetics of biomass growth, substrate utilization and LA production were studied using models of modified Luedeking–Piret, logistic and simple Luedeking–Piret equations, respectively, for the surface and submerged processes. The YPH containing 80 g L−1 of reducing sugars (glucose, xylose, ribose and others) was obtained by acid hydrolysis (H2SO4) of 100 g L−1 of yam peel and used for fermentation. The LA yield from the YPH was 80.03% and 75.63% for the surface and submerged fermentation, respectively. These values were higher than those of synthetic glucose syrup (SGS), which were 77.36% and 38.96% for surface and submerged fermentation, respectively. The models were satisfactory for the fermentation processes with high accuracy of R2 ((0.89 to 0.96), (0.95 to 0.99) and (0.86 to 0.99)) for biomass growth, substrate (SGS/YPH) utilization and LA production, respectively. The models also elucidated that the fermentation process was growth associated (α) instead of non‐growth associated (β) because the value of α/β was greater than 1. This justified the other kinetic parameters obtained for the growth phase. Therefore, Rhizopus oryzae effectively converted YPH to LA with a high yield and purity. © 2021 Society of Industrial Chemistry and John Wiley & Sons Ltd

show abstract

Section: Methodsmentioning

confidence: 99%

\int_{0}^{P} d P = \propto \int_{0 - Δ t}^{t - Δ t} \frac{d X}{d t} d t + β true \int_{0 - Δ t}^{t - Δ t} X d t

According to Mitra and Dutta, 47 the term ‘Δ t ’ represented delay in the LA production with respect to biomass growth.…”

Section: Methodsmentioning

confidence: 99%

\int_{0}^{P} d P = \propto \int_{0 - Δ t}^{t - Δ t} \frac{d X}{d t} d t + β true \int_{0 - Δ t}^{t - Δ t} X d t

\begin{matrix} P = α X_{o} [\frac{e^{μ_{\max} t}}{1 - (\frac{X_{o}}{X_{m}}) (1 - e^{μ_{\max} t})}] \\ + β \frac{X_{m}}{μ_{\max}} \ln [1 - \frac{X_{o}}{X_{m}} (1 - e^{μ_{\max} t})] \end{matrix}

The plot of P against t in a non‐linear curve was used to evaluate the parameters of α and β .…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Lactic acid production: Utilization of yam peel hydrolysate as a substrate using Rhizopus orysae in kinetic studies

Ajala

Onoriemu

et al. 2021

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

show abstract

“…The microorganism Kocuria marina DAGII was isolated from soil during routine screening of pigment producing microorganisms in the department of Biotechnology, NIT Durgapur [11]. This bacteria produces β-cryptoxanthin (β-CRX).…”

Section: Microorganism Cultivation Medium and Pigment Extractionmentioning

confidence: 99%

Role of β-Cryptoxanthin as an Antioxidant and Its Ability to Bind with Transferrin

Ghosh¹,

Hazra²,

Dutta³

2019

IJBBB

Self Cite

View full text Add to dashboard Cite

The potential nutraceutical benefits of carotenoids, which are versatile bioactive compounds, have been of great interest recently for its applications as dietary supplements. Carotenoids are red-orange coloured pigments which absorb light in the wavelength region of 400-550 nm. They are produced by plants, bacteria, algae and fungi and are abundantly distributed in the nature. In this study, our focus is on beta-cryptoxanthin (β-CRX), a yellow colored pro-vitamin A xanthophyll which is extracted from a previously isolated bacterium Kocuria marina DAGII grown in Brain Heart Infusion and sub-cultured in low cost dairy waste like whey and incubated at 25°C and 150 rpm for 5 days. The extracted beta-cryptoxanthin showed good radical scavenging activity and played a role in inhibition of lipid oxidation.Transferrin is a glycoprotein which plays a significant role in the mobilisation of iron in the body. It has two receptors TfR1 and TfR2 amongst which TfR1 binds to the iron-loaded transferrin. In cases of secondary hemochromatosis, HFE protein competes with transferrin to bind to TfR1 which leads to iron built up which is detrimental to the human body. Beta-cryptoxanthin was found to bind to Transferrin with a binding energy of -8.2 kcal/mol.

show abstract

“…(Venugopalan et al, 2013) showed a significant ability to produce canthaxanthin (CX) as their secondary metabolite. CX is an orange-red ketocarotenoid with significant applications in food and feed industry owing to its strong antioxidant property and anticancer, anti-inflammatory and immunomodulatory activities (Mitra et al, 2018). D. maris NITD is a gram-positive, aerobic, non-motile bacterium that has been established as an efficient CX producer (Goswami et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Carotenoid accumulation in Dietzia maris NITD protects from macromolecular damage

Bera¹

2020

bta

View full text Add to dashboard Cite

Over the past decades, nutraceuticals have attracted considerable interest because of their potential nutritional, safety and therapeutic effects. Carotenoid molecules are potent nutraceuticals with an attractive colour and have been shown to play crucial roles in the immune system and susceptibility of organisms to certain diseases. Reactive oxygen species (ROS) are considered to be a major threat to cellular components and lead to protein oxidation, lipid peroxidation and DNA damage. In the present study, canthaxanthin (CX), an orange-red keto-carotenoid, was extracted from Dietzia maris NITD. Carbonyl content measurement showed that the presence of CX can prevent protein oxidation in D. maris NITD. The inhibition of protein oxidation in vitro revealed a high correlation coefficient of the extracted CX with positive controls. Both the linoleic acid model (IC 50 = 932.48 μg/ml) and the goat liver model experiments showed that CX can also inhibit lipid peroxidation in vitro. CX from D. maris NITD was also proved to be effective in maintaining the observable intactness of a model DNA (pBR322). The present study indicated that bacterial CX can be a useful bioactive compound to prevent oxidative damage incurred by cellular macromolecules in terms of pharmaceutical and nutraceutical aspects.

show abstract

Growth profiling, kinetics and substrate utilization of low-cost dairy waste for production of β-cryptoxanthin by Kocuria marina DAGII

Cited by 17 publications

References 51 publications

Lactic acid production: Utilization of yam peel hydrolysate as a substrate using Rhizopus orysae in kinetic studies

Lactic acid production: Utilization of yam peel hydrolysate as a substrate using Rhizopus orysae in kinetic studies

Role of β-Cryptoxanthin as an Antioxidant and Its Ability to Bind with Transferrin

Carotenoid accumulation in Dietzia maris NITD protects from macromolecular damage

Contact Info

Product

Resources

About