2016
DOI: 10.1074/jbc.m116.757450
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Growth Phase-dependent Variation of RNase BN/Z Affects Small RNAs

Abstract: Edited by Charles SamuelRNase BN, the RNase Z family member in E. coli, can participate in the processing of tRNA precursors. However, this function only becomes apparent when other processing enzymes are absent, raising the question of its primary physiological role. Here, we show that RNase BN itself is subject to growth phasedependent regulation, because both rbn mRNA and RNase BN protein are at their highest levels in early exponential phase, but then decrease dramatically and are essentially absent in sta… Show more

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Cited by 19 publications
(22 citation statements)
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“…RNase Z is widely distributed among bacteria (Condon and Putzer, 2002 ). In E. coli RNase Z plays a significant role in mRNA decay (Perwez and Kushner, 2006 ), and controls the levels of 6S RNA, a stable sRNA, and an important transcription regulator that acts by binding to the sigma 70-containing holoenzyme of RNA polymerase (Chen et al, 2016 ).…”
Section: Resultsmentioning
confidence: 99%
“…RNase Z is widely distributed among bacteria (Condon and Putzer, 2002 ). In E. coli RNase Z plays a significant role in mRNA decay (Perwez and Kushner, 2006 ), and controls the levels of 6S RNA, a stable sRNA, and an important transcription regulator that acts by binding to the sigma 70-containing holoenzyme of RNA polymerase (Chen et al, 2016 ).…”
Section: Resultsmentioning
confidence: 99%
“…6S RNA is processed from a larger transcript in E. coli by mechanisms involving RNase BN or the endoribonucleases RNase G and RNase E (Chen, Dutta, & Deutscher, ; Kim & Lee, ) and further trimmed by exoribonucleases RNase T and RNase PH (Li, Pandit, & Deutscher, ). B. burgdorferi has a limited repertoire of ribonucleases, compared to E. coli and B. subtilis , and lacks genes encoding RNase G and RNase E; the only predicted endoribonuclease homologs are RNase III, RNase M5, RNase P, RNase Y, RNase Z, YbeY and RNase HII (Anacker, Drecktrah, LeCoultre, Lybecker, & Samuels, ; Archambault, Borchert, Bergeron, Snow, & Schlax, ; Fraser et al, ).…”
Section: Resultsmentioning
confidence: 99%
“…This is only the second identification of an sRNA-protein interaction in B. burgdorferi (Lybecker, Abel, Feig, & Samuels, 2010;Lybecker & Samuels, 2017). 6S RNA is processed from a larger transcript in E. coli by mechanisms involving RNase BN or the endoribonucleases RNase G and RNase E (Chen, Dutta, & Deutscher, 2016;Kim & Lee, 2004) and further trimmed by exoribonucleases RNase T and RNase PH (Li, Pandit, & Deutscher, 1998 (Anacker, Drecktrah, LeCoultre, Lybecker, & Samuels, 2018;Archambault, Borchert, Bergeron, Snow, & Schlax, 2013;Fraser et al, 1997). The size of Bb6S RNA was assayed by Northern blot in individual RNase mutant strains to investigate Bb6S RNA processing.…”
Section: Re Sultsmentioning
confidence: 99%
“…PGN_0388 is an anti‐oxidant thiol peroxidase, thus the conditional essentiality of an oxidative stress gene is established. The PGN_0770 product is RNase Z, a 6S RNA‐/σ70‐related transcriptional regulator, at least in Escherichia coli (Chen, Dutta, & Deutscher, ). The gene targets of PGN_0770 in P. gingivalis , and their relevance to cigarette smoke, have yet to be ascertained.…”
Section: Discussionmentioning
confidence: 99%