Growth phase‐dependent changes in the subcellular localization of pre‐B‐cell colony‐enhancing factor¹

Abstract

A cDNA encoding the homolog of the human pre-Bcell colony-enhancing factor (PBEF), a cytokine-like secreted protein, was isolated from a rat cDNA library. This protein existed in both the cytoplasm and nucleus of the cells, and the amount was higher in the cytoplasm than in the nucleus of proliferating PC-12 and Swiss 3T3 cells but higher in the nucleus than in the cytoplasm of the PC-12 cells treated with nerve growth factor and the 3T3 cells grown to a con£uent state. Thus, the so-called PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle.

Pre–B cell colony‐enhancing factor/visfatin, a new marker of inflammation in rheumatoid arthritis with proinflammatory and matrix‐degrading activities

“…Overexpression of PBEF in human vascular SMCs induced enhanced survival by its regulatory effect on NADdependent deacetylase activity. Moreover, it has been shown that subcellular localization of PBEF is dependent on the cell cycle, suggesting a role for PBEF in cell cycle regulation (31). Using immunohistochemical analysis, we observed nuclear and cytoplasmic expression of PBEF.…”

The circulating PBEF/NAMPT/visfatin level is associated with a beneficial blood lipid profile

“…Although PBEF has been regarded as an adipose tissue-related hormone, studies show that PBEF is ubiquitously expressed in the body. Among different human tissues, the maximum gene expression was found in the liver and peripheral blood leukocytes [6,22]. A large-scale analysis of the human transcriptome indicated that the PBEF gene is highly expressed in whole blood, over 100 times of the median abundance, while the expression in adipocytes is just about 2 times of the median abundance (GEO series GSE1133) [23].…”

Regulation of Poly(ADP-ribose) Polymerase-1-dependent Gene Expression through Promoter-directed Recruitment of a Nuclear NAD+ Synthase

“…NMN is further converted into NAD ϩ by nicotinamide mononucleotide adenylyltransferase (NMNAT). The mammalian nicotinamide phosphoribosyltransferase enzyme is essential for early embryonic development (6) and has been found in both the cytosol and nucleus (7,8).…”