Growth performance, nutrient digestibility and digestive enzyme activity in Asian seabass,Lates calcariferjuveniles fed diets supplemented with cellulolytic and amylolytic gut bacteria isolated from brackishwater fish

Abstract

Asian seabass, Lates calcarifer juveniles were fed for 30 days to evaluate the efficacy of cellulolytic (Bacillus sp. DDKRC1.) and amylolytic (Bacillus subtilis DDKRC5.) bacteria supplemented diets. Four experimental groups were maintained in triplicate of which group I was the control group and received no bacterial supplementation. Groups II, III and IV were fed diet supplemented with B. subtilis DDKRC5. (14.25 9 10 7 CFU/mL), Bacillus sp. DDKRC1. (2.94 9 10 7 CFU/mL) and mixture of both the microbes (1:1 ratio), respectively, @ 1% (v/w). After 30 days of feeding, fish reared as group IV showed significantly higher (P < 0.01) weight gain (141.42%), survival (91.50%), protein efficiency ratio (1.18) and significantly lower (P < 0.01) FCR (2.15) than that of other groups.Nutrient digestibility parameters were significantly (P < 0.01) higher in group IV followed by groups III, II and I. Digestive cellulase, amylase and protease activities in the gastrointestinal tract were also significantly (P < 0.01) higher in group IV. Amylolytic and cellulolytic microbial count in the gastrointestinal (GI) tract were significantly (P < 0.01) higher in groups II, III and IV as compared with that of control. It can be concluded that feed supplemented with mixture of B. subtilis DDKRC5. and Bacillus sp. DDKRC1. showed better performance in Asian seabass juveniles.

Carbon sources affect water quality and haemato‐biochemical responses ofLabeo rohitain zero‐water exchange biofloc system

“…Several studies are in agreement that bioflocs act as supplemental food source to cultured organisms (Ahmad, Babitha Rani, Verma, & Maqsood, ; Burford, Thompson, McIntosh, Bauman, & Pearson, ; Kuhn, Boardman, Craig, Flick, & McLean, ). Nutrient digestibility is positively correlated with digestive enzyme activity (De, Ghoshal, Ananda Raja, & Kumar, ). Higher activities of digestive enzymes in biofloc treatment groups suggest improved growth performances and enhanced utilization of macromolecules.…”

Evaluation of growth, feed utilization efficiency and immune parameters in tiger shrimp (Penaeus monodon) fed diets supplemented with or diet fermented with gut bacteriumBacillussp. DDKRC1. isolated from gut of Asian seabass (Lates calcarifer)

“…Furthermore, the bacterial supplemented group had significantly higher ( p < .01) PER, improved nutrient digestibility and significantly lower ( p < .01) FCR compared with control. Improved survival and growth were reported after supplementation of probiotics in different shrimp and fish species like Indian white shrimp, Fenneropenaeus indicus (Ziaei‐Nejad et al., ), American white shrimp, Litopenaeus vannamei (Wang, ), Asian seabass (De et al., ), common carp (Wang & Xu, ), rohu (Ghosh, Sen & Ray, ), Japanese flounder (Taoka et al., ), gilthead seabream (Suzer et al., ) and Pursean sturgeon and beluga (Askarian, Kousha, Salma & Ringø, ). However, Shariff, Yosoff, Vevaraja and Srinivasa Rao () found that treatment of commercial Bacillus probiotics in L .…”

“…DDKRC1.‐supplemented shrimp (group II) as compared to shrimp fed control diet and fermented diet (group III). Increased beneficial bacterial population in gut of the bacterial supplemented group leads to increased digestive enzyme (amylase and protease) and degradation enzyme (cellulase) activity due to extracellular secretion in the intestinal tract, better nutrient digestibility and nutrient absorption (Al‐Dohail, Hashim & Aliyu‐Paiko, ; De et al., ), which in turn contributed to the improved weight gain and improved FCR ( p < .01) in P. monodon . Nutrient digestibility of shrimp fed with live Bacillus sp.…”

“…Probiotic agents exert a beneficial effect through enzymatic assistance to digestion and absorption of nutrients in gastrointestinal tract of host (Erasmus, Cook & Coyne, ; Krishnaprakash, Saravanan, Murugesan & Rajagopal, ; Sahu, Swarnakumar, Sivakumar, Thangaradjou & Kannan, ), competition for adhesion sites and resistance to colonization (Balcázar et al., ; Verschuere, Rombaut, Sorgeloos & Verstraete, ), competition for essential nutrients, reduction in gut pH, production of antagonistic compounds against pathogens (De, ; Kesarcodi‐Watson, Kaspar, Lategan & Gibson, ; Ringø & Gatesoupe, ; Verschuere et al., ), enhancement of the immune response and disease resistance (Balcázar et al., ; Rengpipat, Runkpratanporn, Piyatiratitivorakul & Menasaveta, ; Sahu et al., ). In addition, supplementation of beneficial bacteria improve enzyme activity feed digestibility and feed utilization, health and performance in aquatic animals (Balcázar et al., ; De, Ghoshal, Ananda Raja & Kumar, ; Kesarcodi‐Watson et al., ; Ringø & Gatesoupe, ). Bacillus has been widely used as potential probiotics (Ziaei‐Nejad, ), since they secrete a variety of antimicrobial compounds and exoenzymes (Moriarty, , ).…”

Digestibility and digestive enzyme activity in Labeo fimbriatus (Bloch, 1795) fed periphyton grown on sugarcane bagasse

“…In other words, nutrient digestibility is positively correlated with digestive enzyme activity (Hidalgo et al, 1999;De et al, 2015). Among proteases estimated in the present study, activity of total protease and carboxypeptidase B were higher (p<0.05) in fish fed periphyton, while the activity of others showed no difference between fish fed with periphyton and pelleted feed (p>0.05) (Fig.…”