Growth performance, nucleic acids, leptin and adiponectin and their receptor gene expression were significantly affected by feeding different lipid supplementation in GIFT tilapia juveniles

Abstract

The experiment was to investigate the effect of different dietary lipid levels on the growth performance, nucleic acids, leptin and adiponectin as well as their receptor gene expression in juvenile genetically improved farmed tilapia (GIFT, Oreochromis niloticus). Six groups of the juveniles with 40 days of age in triplicate were fed for 90 days using 6 iso‐nitrogen (34% dietary protein) diets. The 6 diets were control diet contained 0.35% of lipid, without lipid supplementation, and the other diets were added different doses of fish oil to make the different dietary lipid levels of 3.35%, 6.35%, 9.35%, 12.35% and 15.35% based on per kg of dried feed. The samples of whole fish and major tissues/organs were randomly taken at 0 and 90 days for determination, respectively. The main results were as follows: compared with the control diet, the diets with different lipid supplementation improved significantly (p < .05) the specific growth rate (SGR), crude fat, nucleic acids, adiponectin receptors 1 and 2 (AdipoRs 1 & 2) gene expression and serum leptin level in juvenile GIFT tilapia. However, the diets with different lipid supplementation reduced significantly (p < .05) the feed conversion ratio (FCR), hepatosomatic index (HSI), crude protein, leptin receptor (LepR) gene expression and serum adiponectin level. There was no significant effect (p > .05) on the survival rate (SR) in the juveniles fed the diets with different lipid supplementation. In conclusion, the diets with different lipid supplementation could affect significantly the SGR, FCR, HIS, nucleic acids, crude fat and protein, leptin and adiponectin levels and their receptor gene expression of the juveniles. The optimal dietary lipid level was 10.52% for SGR and 10.61% for FCR based on the second‐order polynomial regression analysis between dietary lipid levels against the SGR and FCR of the juveniles, respectively, in the experiment.

Growth performance, antioxidant indexes, and the expression of genes were considerably promoted by dietary supplementation of alanyl‐glutamine and vitamin E in juvenile marine medaka in seawater acidification by carbon dioxide

“…Alanyl‐glutamine dipeptide (Yoon et al, 2020; Yu et al, 2016; Zhang et al, 2022) and VE (Noguchi, 2019; Zhou et al, 2013) were reported to improve fish growth. The same results were obtained in marine juvenile cobia ( Rachycentron canadum ) (Ding et al, 2017, 2018) and freshwater farmed tilapia (GIFT, Oreochromis niloticus ) (Ding et al, 2021; Xu, Zheng, et al, 2020). (2) Alanyl‐glutamine dipeptide and/or VE might improve the antioxidant indexes of fish.…”

“…The RNA/DNA ratio could accurately evaluate the short‐term and long‐term growth status of fish (Grimm et al, 2015; Gupta et al, 2015). The ratio of RNA/DNA is an important indicator of growth, well‐being, and nutritional condition in sea fish (Ding et al, 2009; Xu et al, 2009) and freshwater fish (Ding et al, 2021).…”

Effect of replacing a portion of fish meal with unfermented and/or fermented soybean meal on the growth performance, antioxidant and immunity capacity and related gene expression in juvenile Coho Salmon, Oncorhynchus kisutch

“…The expression of antioxidant‐related genes in the livers of the fish was determined following the method of Ding et al (2021). Briefly, 500 ng of total RNA extracted from samples was reverse‐transcribed individually into cDNA using the Steady Pure Universal RNA Extraction Kit and Evo M‐MLV reverse transcription kit (Accurate Biology Bio‐technology Engineering Ltd., Changsha, China).…”

The Effect of Dietary Lipid Supplementation on the Serum Biochemistry, Antioxidant Responses, Initial Immunity, and mTOR Pathway of Juvenile Tilapia (Oreochromis niloticus)

“…Lipid, as one of the most essential nutrients for fish, plays a crucial role in their growth, development, and metabolism [1]. Lipid not only provides energy for fish but also serves as an important component of their bodies [2]. However, both excessive and insufficient levels of dietary lipid supplementation can have inhibitory effects on growth, development, and disease resistance [3].…”

“…Genetic improvement-farmed tilapia (GIFT, Oreochromis niloticus) is an important commercially cultured species worldwide and known for its strong disease resistance, fast growth rate, high nutritional value, and low-cost feed ingredients [2]. Considering the omnivorous nature and economic significance of GIFT tilapia, this study selected GIFT tilapia as the experimental fish.…”