Growth performance and RNA/DNA ratio of noble crayfish (<i>Astacus astacus</i>) and narrow‐clawed crayfish (<i>Pontastacus leptodactylus</i>) fed fish waste diets

Roessler, Tanja Yvonne; Wirtz, Andrea; Slater, Matthew James; Henjes, Joachim

doi:10.1111/are.14655

Cited by 5 publications

(3 citation statements)

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“…Studies have shown that during hibernation, the body's metabolism and transcriptional activity are completely suppressed [7,12,36]. DNA content was not affected by cell processes at different stages, but RNA content varied with the rate of protein synthesis [37], which was consistent with the results of this experiment. Histones are closely associated with and perform an important role in gene transcription and translation [38].…”

Section: Discussionsupporting

confidence: 90%

Induction of Hibernation and Changes in Physiological and Metabolic Indices in Pelodiscus sinensis

Lin

You

et al. 2023

Biology

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Pelodiscus sinensis (P. sinensis) is a commonly cultivated turtle species with a habit of hibernation. To study the changes in histone expression and methylation of P. sinensis during hibernation induction, a model was established by artificial induction. Physiological and metabolic indices were measured, and the expression and localization of histone (H1, H2A, H2B, H3, and H4) and methylation-related genes (ASH2L, KMT2A, KMT2E, KDM1A, KDM1B, and KDM5A) were measured by quantitative PCR, immunohistochemistry, and Western blot analysis. The results indicated that the metabolism, antioxidation index, and relative expression of histone methyltransferase were significantly decreased (p < 0.05), whereas the activity and expression of histone demethyltransferase were significantly increased (p < 0.05). Although our results showed significant changes in physiological and gene expression after hibernation induction, we could not confirm that P. sinensis entered deep hibernation. Therefore, for the state after cooling-induced hibernation, cold torpor might be a more accurate description. The results indicate that the P. sinensis can enter cold torpor through artificial induction, and the expression of histones may promote gene transcription. Unlike histones expressed under normal conditions, histone methylation may activate gene transcription during hibernation induction. Western blot analysis revealed that the ASH2L and KDM5A proteins were differentially expressed in the testis at different months (p < 0.05), which may perform a role in regulating gene transcription. The immunohistochemical localization of ASH2L and KDM5A in spermatogonia and spermatozoa suggests that ASH2L and KDM5A may perform a role in mitosis and meiosis. In conclusion, this study is the first to report changes in histone-related genes in reptiles, which provides insight for further studies on the physiological metabolism and histone methylation regulation of P. sinensis during the hibernation induction and hibernation period.

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Section: Discussionsupporting

confidence: 90%

Induction of Hibernation and Changes in Physiological and Metabolic Indices in Pelodiscus sinensis

Lin

You

et al. 2023

Biology

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“…In addition, co‐farming of crayfish could exist with other fish species as the fish food faeces could react as feed for crayfish with some additional supplements. The above examples constitute a part of a cyclical economy (Roessler et al, 2020). Another aspect of the by‐product's utilization is the aquaponics systems where aquaculture wastewater—which contains unused feed and faeces—can operate as plant sources.…”

Section: Freshwater Crayfish Aquaculturementioning

confidence: 99%

Biology, distribution, conservation status and stocking perspective of freshwater crayfish in Greece: An updated review

Alvanou

Papadopoulos

Lattos

et al. 2022

Aquaculture Research

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Freshwater crayfish are among the most important aquatic inhabitants in terms of ecosystem integrity retention. Although Astacus astacus and Pontastacus leptodactylus are characterized by the IUCN as vulnerable and least concern, respectively, Greek official data and local authorities report them as highly endangered or threatened at local levels. Therefore, all freshwater crayfishing activities from the main producing areas in Greece have been forbidden for 1 year since August 2021. Since aquaculture in Greece remains primarily focused on marine freshwater fish, freshwater crayfish aquaculture establishment is expected to provide a promising alternative for regional economic development and a solution to unemployment. Under this prism, the present study represents a comprehensive review of the most important features related to biology, distribution, aquaculture methods, economic importance and research progress considering both stocking perspectives and conservation strategies of those two crayfish species. Recent measurements by local authorities for the protection of the freshwater crayfish populations are described and evaluated. Freshwater crayfish aquaculture is expected to be beneficial for Greece, a country that covers all requirements for this sector. The updated information regarding these two species, summarizing the existing literature towards aquaculture conditions, poses suggestions and presents future perspectives on this field.

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“…Concerning lipid content, the optimal growth has been observed in a diet lipid content among 10%–13%. Additionally, many by-products and waste ingredients had been added to crayfish diets such as olive mill wastewater ( Parrillo et al, 2017 ), pikeperch faeces ( Roessler et al, 2020 ) and shrimp waste meal ( Bahadir Koca et al, 2011 ) exhibiting ambiguous results on growth and health of crayfish. More specifically, although olive mill wastewater and 10% shrimp waste mill substitution had positive effects on final weight and weight gain, pikeperch faeces failed to increase the growth performance.…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary substitution of fishmeal by black soldier fly (Hermetia illucens) meal on growth performance, whole-body chemical composition, and fatty acid profile of Pontastacus leptodactylus juveniles

et al. 2023

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Freshwater crayfish are considered as aquatic products of high quality and high nutritional value. The increasing demand has led to populations reduction in several locations throughout their range. Thus, the development of appropriate rearing conditions is considered necessary, among which, optimization of their diet is a basic part. Towards this direction, in the present study, a 98-day feeding trial was carried out to evaluate the impact of dietary fishmeal substitution by Hermetia illucens meal on Pontastacus leptodactylus juveniles kept under laboratory conditions. Insect meals represent an environmentally friendly alternative solution, considered as a high-value feed source, rich in nutrients such as protein and fat. Three dietary regimens were utilized with a fishmeal-based without Hermetia meal (HM) defined as the control diet (HM0), and two diets, the first with 50% (HM50) and the second with 100% (HM100) of fishmeal substitution by HM, respectively. Growth performance, whole-body composition, and fatty acid profiles of individuals were studied in the different treatments. At the end of the feeding trial, statistically significant differences were observed in the mean survival rate (SR), specific growth rate (SGR), feed conversion ratio (FCR) and weight gain (WG) values. More specifically, animals fed with HM-based diets had higher mean SR, while the control group performed better regarding FCR and SGR. The HM inclusion in the diet significantly altered the whole-body chemical composition of the crayfish signifying a different metabolic utilization compared to fishmeal (FM). The fatty acid analysis revealed that 16:0 (palmitic acid) was the predominant saturated fatty acid (SFA), 18:1ω9 (oleic acid) was found to be the main monounsaturated fatty acid (MUFA), while 18:2ω6 (linoleic acid) represented the major polyunsaturated fatty acid (PUFA) followed by C20:3 cis ω3 (cis-11-14-17-eicosatrienoate) and C22:6 cis ω3 (cis-4,7,10,13,16,19-Docosahexaenoic) fatty acids. The inclusion of dietary HM significantly reduced the contents of ∑SFAs, ∑PUFAs and ∑ω6 fatty acids, as well as those of C22:6 cis ω3 and increased the ω6/ω3 and hypocholesterolemic to hypercholesterolemic ratios in the body. In parallel with improvements in balanced diets and in culture conditions that need to be optimised for rearing of freshwater crayfish, our study provides new data that enlighten the suitability of insect meals in the nutrition of P. leptodactylus.

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Growth performance and RNA/DNA ratio of noble crayfish (Astacus astacus) and narrow‐clawed crayfish (Pontastacus leptodactylus) fed fish waste diets

Cited by 5 publications

References 35 publications

Induction of Hibernation and Changes in Physiological and Metabolic Indices in Pelodiscus sinensis

Induction of Hibernation and Changes in Physiological and Metabolic Indices in Pelodiscus sinensis

Biology, distribution, conservation status and stocking perspective of freshwater crayfish in Greece: An updated review

Effects of dietary substitution of fishmeal by black soldier fly (Hermetia illucens) meal on growth performance, whole-body chemical composition, and fatty acid profile of Pontastacus leptodactylus juveniles

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