Growth Performance and Intestinal Microbiota Diversity in Pacific White Shrimp Litopenaeus vannamei Fed with a Probiotic Bacterium, Honey Prebiotic, and Synbiotic

Hasyimi, Wahid; Widanarni, Widanarni; Yuhana, Munti

doi:10.1007/s00284-020-02117-w

Cited by 37 publications

(28 citation statements)

References 32 publications

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“…The dominant bacterial group in shrimps fed the four treatments was the phylum Proteobacteria, which has been reported to be highly abundant in both healthy or diseased shrimp (Dai et al, 2018;, and also in shrimp fed probiotics and synbiotics included in the diet (Hasyimi et al, 2020). Differences in the Proteobacteria abundance due to the diet effect may also relate to shrimp size as observed by Dai et al (2018), who found a temporal variation in shrimp gut microbiota composition (between 8 to 9 g).…”

Section: Resultsmentioning

confidence: 74%

“…The increase of species richness in intestinal microbiota in L. vannamei has been also reported with the use of synbiotic composed of galactooligosaccharide and L. plantarum (Huynh et al, 2019), Bacillus sp. probiotic, honey prebiotic or a mix of them (synbiotic) (Hasyimi et al, 2020), suggesting that synbiotics in feed may modulate intestinal bacterial community.…”

Section: Resultsmentioning

confidence: 99%

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Effect of dietary prebiotic inulin and probiotic Bacillus subtilis and Lactobacillus sp., on the intestinal microbiota of white shrimp Litopenaeus vannamei

Rodríguez¹

2021

BIOTECNIA

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Juvenile Litopenaeus vannamei (1.05 ± 0.1 g) were fed during 4-week with four experimental diets: control (Ctrl), inulin as prebiotic (5 g kg-1) (Pre), Bacillus subtilis and Lactobacillus sp. as probiotic (1 x 105 CFU g-1) (Pro), and a mix of inulin + B. subtilis and Lactobacillus sp. (5 g kg-1 + 1 x 105 CFU g-1) (Syn). Shrimps fed Syn diet showed significantly better feed utilization and higher growth than those in control diet (P < 0.05). The probiotic employed induced higher intestinal bacterial richness, whereas inulin in feed induced higher bacterial diversity in shrimp intestine. The most dominant bacterial phylum in the shrimp intestine among treatments was Proteobacteria with abundance ranging between 80 and 84 %. Prebiotic diet (Pre) increased relative abundance of Firmicutes in shrimp intestine (2 %) compared to the rest of the treatments (0.6 %). Nevertheless, when probiotics were included in the feed (Pro and Syn), a reduction between 3 and 13 % in the relative abundance of Vibrio sp. in shrimp intestine was observed respect to the control treatment, which represent an advantage to control potential pathogens of this genus.

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Section: Resultsmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Effect of dietary prebiotic inulin and probiotic Bacillus subtilis and Lactobacillus sp., on the intestinal microbiota of white shrimp Litopenaeus vannamei

Rodríguez¹

2021

BIOTECNIA

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“…The use of prebiotics has been shown to profoundly impact the diversity and richness of the microbiota of L. vannamei . Hasyimi et al 24 reported that using honey as a prebiotic caused an increase in diversity and richness in the intestines of L. vannamei and had a favorable impact on its growth. These results contrast with those reported by Zhou et al 49 , who observed that inulin causes a decrease in the richness and diversity of the microbiota.…”

Section: Discussionmentioning

confidence: 99%

“…Different approaches have modulated shrimp microbiota through diet, improving growth, disease resistance, and productivity. One strategy has been diet supplementation with probiotics, which exert their activity in the shrimp’s digestive tract by inhibiting the proliferation of pathogens, stimulating the immune response, promoting shrimp growth, survival, and nutrient absorption 24 ,] 25 ,] 26 ,] 27 . The second strategy has been through diet supplementation with prebiotics, mainly using polysaccharides such as inulin, amylose, or fructooligosaccharides (FOS), promoting growth, survival, and positive immunological performance, also reducing the relative abundance of potential pathogens 11 , 28 – 30 .…”

Section: Introductionmentioning

confidence: 99%

Agavin induces beneficial microbes in the shrimp microbiota under farming conditions

Ochoa‐Romo

Cornejo‐Granados

López-Zavala

et al. 2022

Sci Rep

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Prebiotics and probiotics have shown a number of beneficial impacts preventing diseases in cultured shrimps. Complex soluble carbohydrates are considered ideal for fostering microbiota biodiversity by fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPS). Here we evaluated the growth performance and microbiota composition of the white shrimp Litopenaeus vannamei after dietary intervention using agavin as a FODMAP prebiotic under farming conditions. Adult L. vannamei were raised at a shrimp farm and the effect of agavin supplemented at 2% (AG2) or 10% (AG10) levels were compared to an agavin-free basal diet (BD). After 28 days-trial, the feed conversion ratio, total feed ingested, and protein efficiency ratio was significantly improved on animals fed with AG2. At the same time, no effect on growth performance was observed in AG10. Surprisingly, after sequencing the V3–V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD. The beta diversity analysis showed a clear significant microbiota clustering by agavin diets only in the hepatopancreas, suggesting that agavin supplementation had a more substantial deterministic effect on the microbiota of hepatopancreas than on the intestine. We analyzed the literature to search beneficial microbes for shrimp’s health and found sequences for 42 species in our 16S data, being significantly increased Lactobacillus pentosus, Pseudomonas putida and Pseudomonas synxantha in the hepatopancreas of the AG10 and Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the hepatopancreas of the AG2, both compared to BD. Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas. In addition, we also sequenced the DNA of agavin and found 9 of the 42 beneficial microbes. From those, Lactobacillus lactis and Lactobacillus delbrueckii were found in shrimps fed with agavin (both AG2 and AG10), and Lysinibacillus fusiformis in AG10 and they were absent the BD diet, suggesting these three species could be introduced with the agavin to the diet. Our work provides evidence that agavin supplementation is associated with an increase of beneficial microbes for the shrimp microbiota at farming conditions. Our study provides the first evidence that a shrimp prebiotic may selectively modify the microbiota in an organ-dependent effect.

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“…The majority of studies dealing with synbiotics are conducted in humans, but over the past few years, increasing research has been focused on farmed aquatic animals ( Hasan et al, 2019 ; Dawood et al, 2020 ). There is evidence to suggest that synbiotics influence the microbial ecology of the intestines of fish and play a role in causing beneficial effects on the health and growth traits of farmed fish, e.g., by preventing the negative effects imposed by infection as well as environmental stress, and by elevating the activities of the digestive enzymes, which eventually contribute to improved feed utilization and growth performances ( Huynh et al, 2017 ; Kumar et al, 2018 ; Mohammadian et al, 2019 ; Hasyimi et al, 2020 ; Moustafa et al, 2020 ; Kong et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19α Gene Expression in Zebrafish Danio rerio

et al. 2021

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In this study, we tested the compatibility of two extracts from the plant Jerusalem artichokes and button mushrooms with two different Lactobacillus probiotics (Lactobacillus acidophilus; La and Lactobacillus delbrueckii subsp. Bulgaricus; Lb) to develop a synbiotic formulation to improve the growth, survival, and reproductive performances of farmed fishes. Initially, we employed in vitro approach to monitor the growth of the probiotic lactobacilli in the presence of the different doses of the plant-based prebiotics, with the aim of selecting interesting combination(s) for further verification under in vivo conditions using zebrafish as a model. Results from the in vitro screening assay in the broth showed that both the probiotic species showed a preference for 50% mushroom extract as a source of prebiotic. A synbiotic formulation, developed with the selected combination of L. acidophilus, L. bulgaricus, and 50% mushroom extract, showed a positive influence on the growth and reproductive performances of the zebrafish. Our findings also imply that the improvement in the reproductive indices was associated with the upregulation of a cyp19a gene. Overall results suggest that a combination of L. acidophilus, L. bulgaricus, and mushroom extract can be considered as a potential synbiotic for the successful production of aquaculture species.

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Growth Performance and Intestinal Microbiota Diversity in Pacific White Shrimp Litopenaeus vannamei Fed with a Probiotic Bacterium, Honey Prebiotic, and Synbiotic

Cited by 37 publications

References 32 publications

Effect of dietary prebiotic inulin and probiotic Bacillus subtilis and Lactobacillus sp., on the intestinal microbiota of white shrimp Litopenaeus vannamei

Effect of dietary prebiotic inulin and probiotic Bacillus subtilis and Lactobacillus sp., on the intestinal microbiota of white shrimp Litopenaeus vannamei

Agavin induces beneficial microbes in the shrimp microbiota under farming conditions

In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19α Gene Expression in Zebrafish Danio rerio

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