Growth on Sabouraud's agar of dermatophytes obtained by conventional scraping and by stripping with vinyl tape

Knudsen, E. A.

doi:10.1111/j.1365-2133.1974.tb06380.x

Cited by 10 publications

(2 citation statements)

References 2 publications

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“…Moreover, lack of keratin, which is considered as a nutrient source of dermatophytes, and a dry environment, can also precipitate fungal death. Scientific data, however, show that there is no substance in the adhesive tape that can inhibit fungal viability 7,8) .…”

Section: Discussionmentioning

confidence: 99%

The Use of Adhesive Tapes to Transfer Skin-scrapings for Sequential Laboratory Diagnosis of Dermatophytosis

Pattanaprichakul

Leeyaphan

Rujitharanawong

et al. 2016

Medical Mycology Journal

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Background: Dermatophytosis usually causes a skin problem, which requires both clinical findings and laboratory investigations for diagnosis. Whereas, fungal culture is considered as the gold standard procedure, it is more difficult to perform compared to potassium hydroxide（KOH）examination. Objective: To evaluate the efficacy of KOH and fungal culture examinations from skin-scraping specimens, which were kept in transparent adhesive tapes at different time intervals. Methods: Skinscraping specimens were collected from clinically suspected cases by conventional technique using scalpel blades and kept using transparent adhesive tapes. KOH（10%）preparation and fungal cultures were then performed by highly experienced technicians at different time intervals: day 0（the day of specimen collection, which was set as the standard reference point） , day 3 or 4, day 7, day 14, and day 28. Thereafter, the yield for detection of fungal elements by KOH examination and positive fungal cultures from specimens stored in adhesive tape was determined at different time intervals and compared to the results from the standard reference point. Results: The sensitivity of KOH examinations from 162 patients at different time intervals showed no significant difference when compared to the standard reference point at day 0. In contrast, the sensitivity of fungal culture progressively decreased as the storage period was prolonged. Conclusions: KOH examination of skin-scrapings from the adhesive tape storage technique yield good results even when specimens have been kept up to one month. On the other hand, the sensitivity of fungal cultures gradually declines as storage time is extended.

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Section: Discussionmentioning

confidence: 99%

The Use of Adhesive Tapes to Transfer Skin-scrapings for Sequential Laboratory Diagnosis of Dermatophytosis

Pattanaprichakul

Leeyaphan

Rujitharanawong

et al. 2016

Medical Mycology Journal

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“…Finally, it might be useful to grow cultures from horny material obtained with the modified skin surface biopsy, by placing polyester tape directly on Sabouraud's dextrose agar. This has been done by Knudsen (1974Knudsen ( , 1975 from stratum corneum strippings with vinyl tape. He obtained a significantly greater number of positive cultures by this technique than by conventional scraping.…”

Section: Discussionmentioning

confidence: 99%

A modified technique (using polyester tape) of skin surface biopsy.

Lachapelle¹,

Gouverneur²,

Boulet³

et al. 1977

Br J Dermatol

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A technical modification of skin surface biopsy has been introduced by using plastic tape instead of glass as holder, mainly to investigate mycological infections of skin folds. Among various brands of plastic sheets, a polyester film (Melinex O UCB-SIDAC) has been demonstrated as the most suitable. A direct microscopic comparison has been made between our modified technique and conventional scraping as procedures for collecting material from interdigital spaces in 30 patients with bilateral athlete's foot. It has been shown that the skin surface biopsy gives a slightly greater number of positive results (presence of dermatophytes or Candida species) than the conventional scraping technique, although the difference between both techniques is not statistically significant at the 0-05 level (0-05 less than P less than 0-10). Some advantages of the modified skin surface biopsy are emphasized.

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A study of dermatophytoses in Bangkok (Thailand)

Imwidthaya

Thianprasit

1988

Mycopathologia

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A total of 719 cases of dermatophytoses was studied in the Division of Dermatology, Department of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand, during January to December 1986. Infection caused by fungus comprises 12%, while the incidence of dermatophytoses was 5.5% of skin disorders. The age group commonly infected was 12-21 years old (mean age of 18 years); 40% was female and 47.5% was male. In the female patients the common prevalent infections were tinea corporis 29%, tinea cruris 23%, tinea pedis 16%, while in male patients it was 28%, 39% and 14% respectively. Only 4 species of dermatophytes were isolated. The prevailing ones were: Trichophyton rubrum 66%, T. mentagrophytes 15%, Epidermophyton floccorum 13% and Microsporum gypseum 6%.

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Growth on Sabouraud's agar of dermatophytes obtained by conventional scraping and by stripping with vinyl tape

Cited by 10 publications

References 2 publications

The Use of Adhesive Tapes to Transfer Skin-scrapings for Sequential Laboratory Diagnosis of Dermatophytosis

The Use of Adhesive Tapes to Transfer Skin-scrapings for Sequential Laboratory Diagnosis of Dermatophytosis

A modified technique (using polyester tape) of skin surface biopsy.

A study of dermatophytoses in Bangkok (Thailand)

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