Growth of Tissue-Engineered Human Nasoseptal Cartilage in Simulated Microgravity

Falsafi, Sassan; Koch, R.

doi:10.1001/archotol.126.6.759

Cited by 28 publications

(28 citation statements)

References 24 publications

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“…Prior investigation has demonstrated that human nasal septal chondrocytes seeded into biodegradable scaffolds demonstrate poor construct structural integrity and promote fibrous rather than cartilaginous ECM deposition. 28,29,30 This is consistent with loss of the chondrogenic phenotype. It has been further noted that tissue…”

Section: Three-dimensional Culture Systemssupporting

confidence: 75%

5 Fundamentals of Tissue Engineering

Papel¹,

Frodel²,

Holt³

et al. 2016

Facial Plastic and Reconstructive Surgery

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Section: Three-dimensional Culture Systemssupporting

confidence: 75%

5 Fundamentals of Tissue Engineering

Papel¹,

Frodel²,

Holt³

et al. 2016

Facial Plastic and Reconstructive Surgery

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“…These scaffolds, usually consisting of poly‐l‐lactic acid, polyglycolic acid, or a copolymer, comprise a woven mesh that traps free chondrocytes. 20 Prior studies involving human nasal septal chondrocytes seeded into biodegradable scaffolds demonstrated a lack of structural integrity 1 and lacked adequate characterization of the differentiated chondrocyte phenotype. 3 In addition, a recent study indicates that polyglycolic acid scaffolds tend to hold human septal chondrocytes in a two‐dimensional rather than in a three‐dimensional array.…”

Section: Discussionmentioning

confidence: 99%

“…Previous research in tissue engineering of nasal septal cartilage has focused on the use of biodegradable scaffolds for neocartilage formation. 1–3 However, these studies describe neocartilage lacking structural integrity. In addition, neocartilage engineered from human septal cells passaged in monolayer loses chondrocyte characteristics.…”

Section: Introductionmentioning

confidence: 99%

Tissue‐Engineered Human Nasal Septal Cartilage Using the Alginate‐Recovered‐Chondrocyte Method

et al. 2004

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“…After decalcification with 5% EDTA-2Na solution for 5 days at 4°C, some specimens were soaked in 20% sucrose-containing PBS prior to embedding into OCT compound for cryostat sectioning (Micron, Heidelberg, Germany), and others were dehydrated chondrocyte differentiation and endochondral bone formation (8,9,23,24,27). More recently, novel strategies of tissue engineering have utilized microgravity (12,19) and hypergravity (17) as aids for optimizing cartilaginous tissue growth. Nowadays, mankind's enterprises in space are no longer science fiction, but significant biological, psychological and environmental problems are yet to be addressed (25).…”

Section: Methodsmentioning

confidence: 99%

Histological assessments on the abnormalities of mouse epiphyseal chondrocytes with short term centrifugal loading

et al. 2007

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We have examined the morphological changes in chondrocytes after exposure to experimental hypergravity. Tibial epiphyseal cartilages of 17-days-old mouse fetuses were exposed to centrifugation at 3G for 16 h mimicking hypergravitational environment (experimental group), or subjected to stationary cultures (control group). Centrifugation did not affect the sizes of epiphyseal cartilage, chondrocyte proliferation, type X collagen-positive hypertrophic zone, and the mRNA expressions of parathyroid hormone-related peptide and fibroblast growth factor receptor III. However, centrifuged chondrocytes showed abnormal morphology and aberrant spatial arrangements, resulting in disrupted chondrocytic columns. Through histochemical assessments, actin filaments were shown to distribute evenly along cell membranes of control proliferative chondrocytes, while chondrocytes subjected to centrifugal force developed a thicker layer of actin filaments. Transmission electron microscopic observations revealed spotty electron-dense materials underlying control chondrocytes' cell membranes, while experimental chondrocytes showed their thick layer. In the intracolumnar regions of the control cartilage, longitudinal electron-dense fibrils were associated with short cytoplasmic processes of normal chondrocytes, indicating assumed cell-tomatrix interactions. These extracellular fibrils were disrupted in the centrifuged samples. Summarizing, altered actin filaments associated with cell membranes, irregular cell shape and disappearance of intracolumnar extracellular fibrils suggest that hypergravity disturbs cell-to-matrix interactions in our cartilage model.The unique mechanical properties of the cartilage are conferred by its avascular composition and the complexity of its extracellular matrices including collagen fibrils, aggrecan, link proteins and so forth. Mesenchymal condensation, chondrocytic differentiation and subsequent matrix synthesis are genetically programmed, and regulated by microenvironmental influences such as soluble mediators, e.g., parathyroid hormone-related peptide (PTHrP) and fibroblast growth factor receptor III (FGFR3) (1, 2, 14). Currently, however, many investigators are paying attention to the fundamental role played by mechanical loading in chondrocyte regulation and cartilage dysfunction. Gravity, the force of attraction between all masses in the universe, can be understood as a source of loading upon chondrocytes and their surroundings. Altered gravity has been shown to affect

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Growth of Tissue-Engineered Human Nasoseptal Cartilage in Simulated Microgravity

Abstract: Once combined with polyglycolic acid scaffolds in the bioreactor cascades that allow efficient seeding and quiescent tissue growth, human septal chondrocytes become a valuable source of reproducible ex vivo cartilage regeneration in the laboratory.

Cited by 28 publications

References 24 publications

5 Fundamentals of Tissue Engineering

5 Fundamentals of Tissue Engineering

Tissue‐Engineered Human Nasal Septal Cartilage Using the Alginate‐Recovered‐Chondrocyte Method

Histological assessments on the abnormalities of mouse epiphyseal chondrocytes with short term centrifugal loading

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