Growth of Tissue-Engineered Human Nasoseptal Cartilage in Simulated Microgravity

Abstract

Once combined with polyglycolic acid scaffolds in the bioreactor cascades that allow efficient seeding and quiescent tissue growth, human septal chondrocytes become a valuable source of reproducible ex vivo cartilage regeneration in the laboratory.

5 Fundamentals of Tissue Engineering

“…Prior investigation has demonstrated that human nasal septal chondrocytes seeded into biodegradable scaffolds demonstrate poor construct structural integrity and promote fibrous rather than cartilaginous ECM deposition. 28,29,30 This is consistent with loss of the chondrogenic phenotype. It has been further noted that tissue…”

Comparison of Scaffolds and Culture Conditions for Tissue Engineering of the Knee Meniscus

“…It has previously been shown that adult cells have a lower synthetic ability than those harvested from young animals. 30,31 Although the modulus of agarose was higher than that of PGA at all measured times, this is most likely due to the material properties and nonbiodegradability of agarose rather than to ECM production by our cells, given the cells in agarose exhibited reduced synthesis compared with PGA. This decrease in the modulus of PGA is consistent with a biodegradable polymer.…”

Cartilage Tissue Engineering Using Cryogenic Chondrocytes

“…The undetectable cutoff point was presumed to be 10 5 cells. The cryogenic cells showed a decaying seeding kinetic comparable with that of fresh chondrocytes 12.…”

“…Their ability to proliferate, differentiate, and form extracellular matrix does not change with time. Histologically, they look similar to cartilage grown from fresh cells, 12 and they have a lag period of 14 days in monolayer culture. The rate of dynamic seeding is similar in cryogenic and fresh cells.…”