Growth of Selected Arboviruses in Serum-Free Suspension Cell Cultures

Tribble, Jr.; Henry, RJ; Nagle, Jr.; Soper, S C

doi:10.21236/ad0448487

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“…20 have previously shown that serum and glutamine were not required for replication of Venezuelan equine encephalomyelitis virus in cells adapted to chemically defined medium. Recently, Nagle and B r o~n~l~~~ showed that Epstein-Barr virus could proliferate in cells grown in chemically defined medium.…”

Section: Discussionmentioning

confidence: 99%

Growth and immunogenicity of foot‐and‐mouth disease virus in baby hamster kidney cells adapted to and continuously grown in a serum‐free chemically defined media

Tomei

Issel

1975

Biotech & Bioengineering

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SummaryThe production of fooband-mouth disease (FMD) virus in baby hamster kidney (BHK) suspension cells grown in serum-free media for subsequent use in vaccines was attempted because of the limited availability of serum in quantities sufficient for propagation of large amounts of cells, as well as the possible presence of mycoplaama, viral contaminants, and interfering antibodies in sera.Suspension cultures (50 to 600 ml) of BHK-21 cells adapted to and continually passed in a glutamine-free autoclavable, chemically defined medium ( B H K S system) were infected with all seven types of FMD virus. Cells were infected at multiplicities of infection (MOI) ranging from 10-l to lo-' plaque-forming units per cell(PFU/cell). The time course of infectious virus release and the amount of complement-fixing (CF) antigen produced were then followed. Peak harvest infectivities of approximately PFU/ml were obtained from 12 to 24 hr after inoculation, depending on input MOI, and were apparently independent of cell concentration over the range 1.5 to 4.0 million cells/ml; the C F endpoint dilutions increased from 1:12 a t the lower cell concentrations to 1:48 at the highest cell concentration. Monovalent and trivalent vaccines have been produced using viruses from the B H K S system, inactivated with acetylethyleneimine and emulsified in oil, and the results of tests in steers and guinea pigs are presented.

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Section: Discussionmentioning

confidence: 99%