Growth of Rhodopseudomonas capsulata under anaerobic dark conditions with dimethyl sulfoxide

Yen, Huei-Che; Marrs, Barry L.

doi:10.1016/0003-9861(77)90246-6

Cited by 149 publications

(65 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Included are three distinct bacteriochlorophyll (Bchl) a pigment-protein complexes: the photochemical reaction center (RC) (36) and the two light-harvesting complexes, designated the B800-850 and B-875 spectral complexes (7,9). When grown anaerobically in the dark, in the presence of alternative electron acceptors, the gratuitous synthesis of the ICM occurs (53). Lee and Kaplan (28) provided the first demonstration that a relatively simple switching mechanism must be involved in the repression of photosynthesis gene expression by oxygen in the genus Rhodobacter when they isolated the spontaneous mutant T 1a , which accumulates photosynthetic membranes in the presence of high oxygen.…”

mentioning

confidence: 99%

Oxygen-insensitive synthesis of the photosynthetic membranes of Rhodobacter sphaeroides: a mutant histidine kinase

Eraso¹,

Kaplan²

1995

J Bacteriol

121

204

View full text Add to dashboard Cite

Two new loci, prrB and prrC, involved in the positive regulation of photosynthesis gene expression in response to anaerobiosis, have been identified in Rhodobacter sphaeroides. prrB encodes a sensor histidine kinase that is responsive to the removal of oxygen and functions through the response regulator PrrA. Inactivation of prrB results in a substantial reduction of photosynthetic spectral complexes as well as in the inability of cells to grow photosynthetically at low to medium light intensities. Together, prrB and prrA provide the major signal involved in synthesis of the specialized intracytoplasmic membrane (ICM), harboring components essential to the light reactions of photosynthesis. Previously, J. K. Lee and S. Kaplan (J. Bacteriol. 174:1158-1171, 1992) identified a mutant which resulted in high-level expression of the puc operon, encoding the apoproteins giving rise to the B800-850 spectral complex, in the presence of oxygen as well as in the synthesis of the ICM under conditions of high oxygenation. This mutation is shown to reside in prrB, resulting in a leucine-to-proline change at position 78 in mutant PrrB (PRRB78). Measurements of mRNA levels in cells containing the prrB78 mutation support the idea that prrB is a global regulator of photosynthesis gene expression. Two additional mutants, PRRB1 and PRRB2, which make two truncated forms of the PrrB protein, possess substantially reduced amounts of spectral complexes. Although the precise role of prrC remains to be determined, evidence suggests that it too is involved in the regulatory cascade involving prrB and prrA. The genetic organization of the photosynthesis response regulatory (PRR) region is discussed.Rhodobacter sphaeroides is a purple, nonsulfur photoheterotrophic bacterium whose mode of growth depends on the concentration of oxygen in the surrounding environment (49). At high oxygen concentrations, R. sphaeroides grows chemoheterotrophically, and morphologically it resembles a typical gram-negative bacterium (3). Under low oxygen or anaerobiosis, the photosynthetic membrane system, designated the intracytoplasmic membrane (ICM), is induced; the ICM fills the interior of the cell, and its abundance is inversely related to the incident light intensity (25). The ICM contains all of the structural and functional components of the photosynthetic apparatus. Included are three distinct bacteriochlorophyll (Bchl) a pigment-protein complexes: the photochemical reaction center (RC) (36) and the two light-harvesting complexes, designated the B800-850 and B-875 spectral complexes (7, 9). When grown anaerobically in the dark, in the presence of alternative electron acceptors, the gratuitous synthesis of the ICM occurs (53).Lee and Kaplan (28) provided the first demonstration that a relatively simple switching mechanism must be involved in the repression of photosynthesis gene expression by oxygen in the genus Rhodobacter when they isolated the spontaneous mutant T 1a , which accumulates photosynthetic membranes in the presence of high oxygen. Subsequen...

show abstract

mentioning

confidence: 99%

Oxygen-insensitive synthesis of the photosynthetic membranes of Rhodobacter sphaeroides: a mutant histidine kinase

Eraso¹,

Kaplan²

1995

J Bacteriol

121

204

View full text Add to dashboard Cite

show abstract

“…Strains were grown on malate-minimal RCV medium (Weaver et al, 1975) in 250 ml side-arm flasks at 30 "C or in RCV supplemented with 50 mM-DMSO, 0.6% glucose and 0.5% pyruvate (RCV+). Neither glucose nor pyruvate supports fermentative growth of R. capsulatus, but they greatly enhance dark anaerobic growth when DMSO is added to RCV medium (Yen & Marrs, 1977). Overnight dark inocula were sparged with N2/02/C02 (78 : 20 : 2, by vol).…”

Section: Methodsmentioning

confidence: 99%

“…It grows photosynthetically in an illuminated environment only when oxygen tension drops below 3% (Oelze & Drews, 1972). Furthermore, in the dark, R. capsulatus is capable of anaerobic growth on nonfermentable sources of carbon when the medium is supplemented by either dimethylsulphoxide (DMSO) or trimethylamine-N-oxide (TMAO) (Yen & Marrs, 1977 ;Cox et al, 1980;Madigan etal., 1980;Schultz & Weaver, Abbreviations : BChl, bacteriochlorophyll; LH, Light-harvesting complex; PS, photosynthetic; RC, reaction-center; RCV medium, DLmalate as the carbon source, ammonium sulphate as the nitrogen source, phosphate buffer, thiamine hydrochloride, and mineral salts; RCV+ medium, RCV supplemented with 50 mM-DMSO, 0.6% glucose and 0.5% pyruvate.…”

Section: Introductionmentioning

confidence: 99%

“…These alternative terminal oxidants stimulate the growth of non-photosynthetic cultures and allow the gratuitous formation of an intracytoplasmic photosynthetic membrane (Yen & Marrs, 1977). Thus it is possible to propagate nonphotosynthetic mutants in conditions in which they can express components of the photosynthetic apparatus, but where photosynthetic growth is not required.…”

Section: Introductionmentioning

confidence: 99%

“…The pattern of photosynthetic gene expression during microaerophilic respiratory growth with an alternative terminal oxidant has not been previously studied. The observation that an intracytoplasmic membrane developes during dark anaerobic growth (Yen & Marrs, 1977) implies that genes for the photosynthetic polypeptide components of the integral membrane BChl-protein complexes are expressed to some extent. We wished to determine whether bacterial cultures maintained oxygen regulation of photosynthetic gene expression in the presence of an alternative terminal oxidant.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Photosynthetic genes in Rhodobacter capsulatus can be regulated by oxygen during dark respiratory growth with dimethylsulphoxide

Leach

Armstrong

Hearst

1991

Journal of General Microbiology

View full text Add to dashboard Cite

In the purple non-sulphur bacterium Rhodobacter capsulatus, genes encoding structural polypeptides of the lightharvesting (LH) and reaction center (RC) complexes incorporated into an intracytoplasmic photosynthetic membrane are induced upon lowering the oxygen tension in the media of aerobically growing cultures. When cultures are grown microaerophilically in the dark, an intracytoplasmic photosynthetic membrane develops gratuitously if a terminal oxidant, such as dimethylsulphoxide (DMSO) is present in the medium. The purpose of the present study was to determine whether in these conditions, photosynthetic genes are completely derepressed or whether they are still inducible in response to a lowering of oxygen tension. Oxygen induction of mRNA for thepuf and puc operons was compared in dark aerobic cultures (20% 0,) shifted to low oxygen conditions (3% 0,) allowing growth microaerophilically with or without DMSO as an accessory terminal oxidant. The extent of the induction was similar in both growth conditions, 6 to 12-fold forpufA mRNA and at least 400-fold forpucBmRNA which encode the light-harvesting I (LHIa) and light-harvesting I1 (LHIIP) polypeptides, respectively. The puf and puc operons were also induced by low oxygen tension in a mutant strain blocked in an early step of bacteriochlorophyll (BChl) synthesis, suggesting that the presence of BChl may not be a prerequisite for the normal oxygen regulation of the genes encoding the structural polypeptides of the photosynthetic apparatus.

show abstract

Rhodobacter

Imhoff¹

2015

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

Rho.do.bac' ter . Gr. n. rhodon the rose; M.L. masc. n. bacter equivalent of Gr. neut. n. bakterion a rod; M.L. masc. n. Rhodobacter red rod. Proteobacteria / Alphaproteobacteria / Rhodobacterales / Rhodobacteraceae / Rhodobacter Cells are ovoid or rod‐shaped , motile by polar flagella or non‐motile, divide by binary fission or budding, may produce capsules and slime, and may form chains of cells. Gram negative ; belong to the Alphaproteobacteria . Phototrophically grown cells form vesicular or lamellar internal photosynthetic membranes . The color of phototrophic cultures is yellow‐green to yellow‐brown, while aerobic cultures are pink to red. Photosynthetic pigments are bacteriochlorophyll a ( esterified with phytol ) and carotenoids of the spheroidene series . Ubiquinone 10 is the major quinone . Major fatty acids are C 18 and C 16 saturated and monounsaturated fatty acids with C 18 : 1 as predominant component. The mol % G + C of the DNA is : 64.4–73.2. Type species : Rhodobacter capsulatus (Molisch 1907) Imhoff, Trüper and Pfennig 1984, 342 ( Rhodonostoc capsulatum Molisch 1907, 23; Rhodopseudomonas capsulata (Molisch 1907) van Niel 1944, 92.)

show abstract

Growth of Rhodopseudomonas capsulata under anaerobic dark conditions with dimethyl sulfoxide

Cited by 149 publications

References 13 publications

Oxygen-insensitive synthesis of the photosynthetic membranes of Rhodobacter sphaeroides: a mutant histidine kinase

Oxygen-insensitive synthesis of the photosynthetic membranes of Rhodobacter sphaeroides: a mutant histidine kinase

Photosynthetic genes in Rhodobacter capsulatus can be regulated by oxygen during dark respiratory growth with dimethylsulphoxide

Rhodobacter

Contact Info

Product

Resources

About