Growth of Ralstonia eutropha on inhibitory concentrations of phenol: diminished growth can be attributed to hydrophobic perturbation of phenol hydroxylase activity

Use of Statistically-Based Design of Experiment to Study Olive-Oil Mill Wastewater Biodegradation by Ralstonia Eutropha

“…Phenol localization in the lipid fraction of the cell membrane changes the membrane fluidity, and in this way phenol exerts its toxic effect on cell growth. Changes in the cell membrane integrity are also related to the inhibitory effect of phenol on the phenol hydroxylase enzyme, which is putatively associated with the cell membrane [21]. The phenolic tolerance of R. eutropha in the present work confirmed the successful performance of the adaptation process.…”

Production of polyhydroxyalkanoates from dephenolised and fermented olive mill wastewaters by employing a pure culture of Cupriavidus necator

“…Taken together, those evidences demonstrated an inhibitory effect due to the complex matrix of the employed substrate. Among others, two groups of chemicals could have exerted inhibitory effects, namely: polyphenols, which can modify the membrane fluidity [37,38], and VFAs, since they can interfere with the proton gradient mechanism [39]. Importantly, the experiment carried out with VFA solutions allowed to exclude VFAs as the main responsible of the former observed phenomenon: indeed, PHAs accumulation was observed under all conditions, including when Sim VFA OMW Acid represented 75% and 100% of the accumulation medium, while corresponding OMW Acid amounts did not allowed significant PHA productions.…”

Modeling of batch phenol biodegradation in internal loop airlift bioreactor with gas recirculation by Candida tropicalis

“…Liquid cultures were grown in the mineral salt medium supplemented with 500 mg/L phenol at 30 • C in a rotary shaker at 200 rpm. Phenol was filtersterilized through membranes (pore size of 0.2 m) and added to the medium before inoculation (Kibret et al, 2000;Léonard and Lindley, 1999). For each batch phenol biodegradation experiment, after washing with sterilized deionized water and sterilized mineral salt medium, 11.3 L mineral salt medium containing the initial phenol concentration of 2000 mg/L and cell concentration of 50 mg/L was added into the bioreactor.…”