Growth of Pseudomonas and Bacillus biofilms on pretreated polypropylene surface

Arkatkar, Ambika; Juwarkar, Asha A.; Bhaduri, Sumit; Uppara, Parasu Veera; Doble, Mukesh

doi:10.1016/j.ibiod.2010.06.002

Cited by 182 publications

(83 citation statements)

References 33 publications

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“…Log phase seed culture (1% of overnight grown culture containing 107 cells) was added to these flasks and they were kept under shaking at 180 rpm at 35-37°C (Scigenics Biotech, Chennai, India). Sea water containing polymer without the microorganism was maintained as positive control and organism without the polymer served as a negative control [2,31].…”

Section: In Vitro Biodegradation Studiesmentioning

confidence: 99%

Biodegradation of Starch Blended High Density Polyethylene using Marine Bacteria Associated with Biofilm Formation and its Isolation Characterization

Muthukumar¹

2014

J Microb Biochem Technol

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The biofouling and biodegradation of starch blended high density polyethylene (HDPE) was studied by immersing it in Bay of Bengal (India) for a period of six months. A positive correlation was observed amongst the various constituents in the biofilm. FTIR spectrum showed the formation of C-O stretching band and decrease in ester and keto carbonyl bands indicating biodegradation. 17% weight loss was observed, while the polymer surface turned hydrophilic. Twenty two bacterial strains were isolated from the biofilm and biochemically characterized. 16sRNA sequence analysis was performed for three strains. In vitro biodegradation of the polymer exposed to sunlight for 150 days incubated with the isolated pure strain (Exiguobacterium) and combination of two strains (Exiguobacterium and B. subtilis) for 75 days, showed a gravimetric weight loss of 4.7 and 12.1% respectively indicating synergy between the two organisms. The current study indicated, the isolated microorganisms could degrade starch blended HDPE.

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Section: In Vitro Biodegradation Studiesmentioning

confidence: 99%

Biodegradation of Starch Blended High Density Polyethylene using Marine Bacteria Associated with Biofilm Formation and its Isolation Characterization

Muthukumar¹

2014

J Microb Biochem Technol

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“…Peaks around 1720-1730 and 1640 cm -1 regions may be due to groups of carboxylic of ester and aldheyde or ketone or even to the presence of double bonds 47 . According to Arkatkar et al (2010) 48 , the formation of the keto carbonyl and the ester carbonyl groups indicate oxidation of the polymer. Formation of carbonyl groups (1723 cm -1 ) by means of sample oxidation in the landfill stimulates microorganisms to initiate the biodegrading process.…”

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confidence: 99%

“…According to Arkatkar (2010) 48 , the ability of a microorganism to utilize any substrate depends on its growth and its adherence to form a biofilm.…”

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confidence: 99%

Degradation study of polypropylene (PP) and bioriented polypropylene (BOPP) in the environment

et al. 2011

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Polymers are vastly employed for numerous purposes in different industrial segments and generate soaring quantities of discarding in the environment. This research analyzed the degradability/biodegradability of polypropylene films (PP) and Bioriented polypropylene (BOPP) polymers after 11 months interred in the São Giácomo landfill in Caxias do Sul. Comparing the buried PP film to a sample of virgin PP, two peaks of degrading activity appeared at the TG curve as well as structure modification typified by occurrence of new absorption bands at FTIR, which can be credited to changes in crystallinity. Thermal analysis carried out on the buried PP and BOPP showed decreases in the percentage of crystallinity due to chain scission. The major reduction was observed in the PP, since its crystallinity is a consequence of polymerization instead of chain orientation processes, as in BOPP. Cracks and erosion of the polymer surface were detected in both PP and BOPP, indicating degrading processes by microorganisms.

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“…However, its brittleness and low heat distortion temperature limit the application of neat PLLA [17][18][19]. Some of the previous studies also reported that Pseudomonas stutzeri bacterium shows potential to form biofilm, i.e., attachment of microorganisms to polymer surface, on the surface of PP [20,21].…”

Section: Introductionmentioning

confidence: 99%

Physico-mechanical characterization and biodegradability behavior of polypropylene/poly(L-lactide) polymer blends

Jain

Madhu

Bhunia

et al. 2014

Journal of Polymer Engineering

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Partially biodegradable polymer films from the blends of polypropylene (PP) and poly(L-lactide) (PLLA) were prepared in an internal mixer by melt blending technique, with and without compatibilizer, maleic anhydride grafted polypropylene (MAPP), followed by compression molding. With regard to tensile properties, 80/20 (PP/ PLLA) and 80/20/6 (PP/PLLA/MAPP) were found as the optimum blends with best combination of the ingredients. Therefore, the blend samples, namely, PP80 (80% PP+20% PLLA) and PP80C6 (80% PP+20% PLLA+6 phr MAPP) were selected as 'optimized' blends and further characterized for their physical, chemical, morphological, and thermal properties. X-ray diffraction studies showed that neat PP and PP80C6 had the same crystallite size indicating compatibility between PP and PLLA due to MAPP. Fourier transform infrared spectroscopy and scanning electron microsopy investigations revealed that the two polymers were completely immiscible in absence of the compatibilizer. Bacterial biodegradation of the samples was performed by exposure to Pseudomonas stutzeri for 60 days and measured in terms of weight loss, optical density, and thermal stability of the samples before and after degradation. The results showed that 80/20 (PP/ PLLA) blends undergo considerable degradation. Reduction in thermal stability of the film samples was also observed through thermogravimetric analysis, which was useful in accelerating their biodegradation.

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Growth of Pseudomonas and Bacillus biofilms on pretreated polypropylene surface

Cited by 182 publications

References 33 publications

Biodegradation of Starch Blended High Density Polyethylene using Marine Bacteria Associated with Biofilm Formation and its Isolation Characterization

Biodegradation of Starch Blended High Density Polyethylene using Marine Bacteria Associated with Biofilm Formation and its Isolation Characterization

Degradation study of polypropylene (PP) and bioriented polypropylene (BOPP) in the environment

Physico-mechanical characterization and biodegradability behavior of polypropylene/poly(L-lactide) polymer blends

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