Growth of Paracoccidioides brasiliensis yeast phase in a chemically defined culture medium

Restrepo, Ángela; Jiménez, Beatriz Elena

doi:10.1128/jcm.12.2.279-281.1980

Cited by 202 publications

(72 citation statements)

References 10 publications

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“…Subsequently, cells were scraped from plates and washed with distilled water. The cells (0.75 g) were transferred to 25 mL McVeigh & Morton (MMcM) liquid minimal medium [49] and incubated at 36°C. The media either contained glucose (0.11 m), potassium acetate (0.28 m), glycolic acid (0.28 m) or ethanol (0.43 m) as carbon sources.…”

Section: Antibody Productionmentioning

confidence: 99%

Phosphorylation is the major mechanism regulating isocitrate lyase activity in Paracoccidioides brasiliensis yeast cells

et al. 2011

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The glyoxylate cycle plays an essential role for anaplerosis of oxaloacetate during growth of microorganisms on carbon sources such as acetate or fatty acids and has been shown to contribute to virulence of several pathogens. Here, we investigated the transcriptional and post‐translational regulation of the glyoxylate cycle key enzyme isocitrate lyase (PbICL) in the human pathogenic fungus Paracoccidioides brasiliensis. Although sequence analyses on fungal isocitrate lyases revealed a high phylogenetic conservation, their regulation seems to differ significantly. Closely related Aspergillus species regulate the glyoxylate cycle at the transcriptional level, whereas Pbicl was constitutively expressed in yeast cells. However, only low PbICL activity was detected when cells were grown in the presence of glucose. Two‐dimensional gel analyses with subsequent antibody hybridization revealed constitutive production of PbICL, but low PbICL activity on glucose coincided with extensive protein phosphorylation. Since an in vitro dephosphorylation of PbICL from glucose grown cells strongly increased ICL activity and resembled the phosphorylation pattern of highly active acetate grown cells, post‐translational modification seems the main mechanism regulating PbICL activity in yeast cells. In agreement, a transfer of yeast cells from glucose to acetate medium increased PbICL activity without requirement of de novo protein synthesis. Thus, inactivation of PbICL by phosphorylation is reversible, denoting a new strategy for the rapid adaptation to changing environmental conditions.

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Section: Antibody Productionmentioning

confidence: 99%

Phosphorylation is the major mechanism regulating isocitrate lyase activity in Paracoccidioides brasiliensis yeast cells

et al. 2011

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“…8 For the testing proposed by NCCLS, the isolates were tested by a broth macrodilution technique with RPMI medium containing L-glutamine, in the absence of sodium bicarbonate and buffered to pH 7.0 with 0.165 mol l )1 morpholinopropanesulfonic acid (Sigma). For Shadomy et al procedure, 9 twofold serial dilutions of each drug stock solution were made using McVeigh & Morton synthetic medium (MVM) 10 as the diluent. In both cases, the final drug concentrations ranged from 0.031 to 64 lg ml )1 .…”

Section: Determination Of Micmentioning

confidence: 99%

Antifungal susceptibility of clinical and environmental isolates of Cryptococcus neoformans to four antifungal drugs determined by two techniques

Moraes

Prímola

Hamdan

2003

Mycoses

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A total of 64 Cryptococcus neoformans strains, including clinical and environmental Brazilian isolates var. neoformans and var. gattii, were tested for susceptibility to amphotericin B, 5-flucytosine, fluconazole and itraconazole. The tests were performed according to the recommendations of National Committee of Clinical Laboratory Standards and the method of macrodilution in liquid medium of Shadomy et al. [Manual de Microbiologia Clínica, 4th ed. Buenos Aires: Editorial Medica Panamericana, 1987: 1229-38]. For most drugs there was a significant difference between the readings taken at 24 and 48 h with both methods. When the minimum inhibitory concentrations obtained by the two techniques were compared, significant differences were observed for amphotericin B and fluconazole. Overall, differences in drug susceptibility with respect to the origin of the isolates or the variety of the fungus were not observed. As an exception, the gattii variety exhibited a high resistance rate to amphotericin B when the technique of Shadomy et al. was applied, a fact possibly related to the greater difficulty for treatment of the disease caused by this fungal variety.

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“…Several mono-and disaccharides can be taken up (D-glucose, D-fructose, D-galactose, D-mannose, D-sorbitol, D-mannitol, D-inositol, a-trehalose, sucrose), except L-sorbose, L-fucose and L-lactose. In order to validate the transcriptome data, two isolates of P. brasiliensis, Pb01 and Pb18, were grown in McVeigh-Morton minimum medium with adaptations [58] and containing different carbon sources, including ethanol and glycogen [54]. The experiment confirmed the data of carbon sources uptake obtained in the transcriptome analysis, except for L-lactose, which is also consumed by P. brasiliensis [54].…”

Section: Mycelium-exclusive Genesmentioning

confidence: 52%

Functional genome of the human pathogenic fungusParacoccidioides brasiliensis

Felipe

Torres

Maranhão

et al. 2005

FEMS Immunology &Amp; Medical Microbiology

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Paracoccidioides brasiliensis is a dimorphic and thermo-regulated fungus which is the causative agent of paracoccidioidomycosis, an endemic disease widespread in Latin America. Pathogenicity is assumed to be a consequence of the cellular differentiation process that this fungus undergoes from mycelium to yeast cells during human infection. In an effort to elucidate the molecular mechanisms involved in this process a network of Brazilian laboratories carried out a transcriptome project for both cell types. This review focuses on the data analysis yielding a comprehensive view of the fungal metabolism and the molecular adaptations during dimorphism in P. brasiliensis from analysis of 6022 groups, related to expressed genes, which were generated from both mycelium and yeast phases.

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Growth of Paracoccidioides brasiliensis yeast phase in a chemically defined culture medium

Abstract: A slight modification of the chemically defined medium of McVeigh and Morton resulted in an excellent substratum for the cultivation of Paracoccidioides brasiliensis yeast phase.

Cited by 202 publications

References 10 publications

Phosphorylation is the major mechanism regulating isocitrate lyase activity in Paracoccidioides brasiliensis yeast cells

Phosphorylation is the major mechanism regulating isocitrate lyase activity in Paracoccidioides brasiliensis yeast cells

Antifungal susceptibility of clinical and environmental isolates of Cryptococcus neoformans to four antifungal drugs determined by two techniques

Functional genome of the human pathogenic fungusParacoccidioides brasiliensis

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