Growth of Microthrix parvicella in nutrient removal activated sludge plants: studies of in situ physiology

Andreasen, Kjær; Nielsen, Per Halkjær

doi:10.1016/s0043-1354(99)00319-x

Cited by 83 publications

(82 citation statements)

References 23 publications

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“…Hence, our results support previous suggestions indicating that "Ca. Microthrix parvicella" is able to take up oleic acid under anaerobic conditions and form storage compounds (e.g., lipids) without initiating balanced growth (4,28). The stored oleic acid or oleic acid derivatives are then able to support cell growth if oxygen becomes available (4,28).…”

Section: Discussionmentioning

confidence: 99%

“…"Ca. Microthrix parvicella" is common in activated sludge wastewater treatment systems, where it causes serious foam problems (4,7). Unfortunately, the organism is hard to grow and the physiology is poorly understood which has made it difficult to establish efficient control measures.…”

mentioning

confidence: 99%

“…Different in situ techniques including the traditional MAR approach has shown that "Ca. Microthrix parvicella" grows mainly (or only) on long-chain fatty acids such as oleic acid (4,28). Furthermore, "Ca.…”

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confidence: 99%

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Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of ¹⁴ CO ₂

Hesselsøe

Nielsen

Roslev

et al. 2005

Appl Environ Microbiol

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14 CO 2 assimilation. This is a metabolic feature that has not been described previously for filamentous bacteria. Such information could not have been derived by using the traditional MAR procedure, whereas the new HetCO 2 -MAR approach differentiates better between substrate uptake and substrate metabolism that result in growth. The HetCO 2 -MAR results were supported by stable isotope analysis of 13 C-labeled phospholipid fatty acids from activated sludge incubated under aerobic and anaerobic conditions in the presence of 13 CO 2 . In conclusion, the novel HetCO 2 -MAR approach expands the possibility for studies of the ecophysiology of uncultivated microorganisms.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of ¹⁴ CO ₂

Hesselsøe

Nielsen

Roslev

et al. 2005

Appl Environ Microbiol

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“…Also, potential use of other electron acceptors was tested by studying uptake of the same organic substrates with nitrate or nitrite present as electron acceptor or under strict anaerobic conditions (no oxygen, nitrate or nitrite present). A pre-incubation step of 2 h was included with unlabelled organic substrate (2 mM) to ensure that only bacteria able to take up large quantities of the substrate under these conditions (for storage or growth) would be MAR-positive (Andreasen & Nielsen, 2000). For analysis of potential uptake for all probe-defined Bacteroidetes, a minimum of 30 filaments of each probe-defined population was investigated in each incubation.…”

Section: Methodsmentioning

confidence: 99%

Identity, abundance and ecophysiology of filamentous bacteria belonging to the Bacteroidetes present in activated sludge plants

Kragelund

Levantesi

Borger³

et al. 2008

Microbiology

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Filamentous members of the Bacteroidetes are commonly observed in activated sludge samples originating from both municipal and industrial wastewater treatment plants (WWTP), where they occasionally can cause bulking. Several oligonucleotide 16S rRNA-targeted probes were designed to target filaments with a needle-like appearance similar to Haliscomenobacter hydrossis. The design of these probes was based on an isolate and a sequence obtained from a micromanipulated filament. The abundance of filamentous Bacteroidetes was determined in 126 industrial samples applying already published and the newly developed probes. Small populations were found in 62 % of the WWTP investigated. However, only relatively few WWTP (13 %) contained large populations of filamentous Bacteroidetes potentially responsible for bulking incidences. The identity of the most abundant filamentous Bacteroidetes with H. hydrossis morphology could be detected by probes CFB719, SAP-309 and the newly designed probe HHY-654. A comprehensive study on the ecophysiology of probe-defined Bacteroidetes populations was conducted on Danish and Czech samples. The studies revealed that they were specialized bacteria involved in degradation of sugars, e.g. glucose and N-acetylglucosamine, and may participate in the conversion of lipopolysaccharides and peptidoglycan liberated by decaying cells. Many surface-associated exo-enzymes were excreted, e.g. chitinase, glucuronidase, esterase and phosphatase, supporting conversion of polysaccharides and possibly other released cell components. The role of filamentous bacteria with a H. hydrossis-like morphology in the activated sludge ecosystem is discussed.

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“…Additional in situ based validation is also essential to understand their ecology as organisms are known to be more specialized than their genome annotation data or behavior in axenic culture would suggest (for example, Kindaichi et al, 2013;McIlroy et al, 2015a). Combining genomic-based investigations and in situ analyses proved to be powerful tools in determining the ecology of 'Candidatus M. parvicella' (Andreasen and Nielsen, 2000;McIlroy et al, 2013;Muller et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge

et al. 2016

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Overgrowth of filamentous bacteria in activated sludge wastewater treatment plants (WWTPs) leads to impaired sludge settleability, a condition known as bulking, which is a common operational problem worldwide. Filaments with the Eikelboom 0092 morphotype are commonly associated with such bulking episodes. Members of the uncultured B45 phylotype, which is embraced within the phylum Chloroflexi, were recently shown to exhibit this morphology. Although these organisms are among the most abundant populations recorded in activated sludge processes, nothing is known about their metabolic characteristics. In this study, a genome sequence, representing the B45 phylotype, was retrieved from a metagenome generated from an activated sludge WWTP. The genome consisted of two chromosomes and one plasmid, which were 4.0, 1.0 and 0.04 Mbps in size, respectively. A metabolic model was constructed for this organism, based on annotation of its genome, showing its ability to generate energy by respiration, utilizing oxygen, nitrite or nitrous oxide as electron acceptors, or by fermentation of sugars. The ability of B45 members to ferment sugars under anaerobic conditions was validated in situ with microautoradiography-fluorescence in situ hybridization. The provisional name of 'Candidatus Promineofilum breve' is proposed for this species. This study represents the first detailed information on an uncultured genus of filamentous organisms from activated sludge.

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Growth of Microthrix parvicella in nutrient removal activated sludge plants: studies of in situ physiology

Cited by 83 publications

References 23 publications

Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of ¹⁴ CO ₂

Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of ¹⁴ CO ₂

Identity, abundance and ecophysiology of filamentous bacteria belonging to the Bacteroidetes present in activated sludge plants

Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge

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Growth of Microthrix parvicella in nutrient removal activated sludge plants: studies of in situ physiology

Cited by 83 publications

References 23 publications

Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of 14 CO 2

Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of 14 CO 2

Identity, abundance and ecophysiology of filamentous bacteria belonging to the Bacteroidetes present in activated sludge plants

Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge

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Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of ¹⁴ CO ₂

Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of ¹⁴ CO ₂