Growth of Listeria monocytogenes is promoted at low temperature when exogenous unsaturated fatty acids are incorporated in its membrane

Touche, Cécile; Hamchaoui, Sarah; Quilleré, Aurore; Darsonval, Maud; Dubois‐Brissonnet, Florence

doi:10.1016/j.fm.2022.104170

Cited by 9 publications

(7 citation statements)

References 39 publications

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“…The previously reported QAC MIC values vary significantly between studies due to differences in the biocide and/or inoculum concentrations, variations in media and assay conditions (27,28,29,54,55). For instance, L. monocytogenes exhibits different physiological traits at lower temperatures (e.g., 15°C) as compared to 37°C, such as the different types of motility and having different membrane fatty acid composition (43). An important aspect of our large-scale study is the consistency and reliability of the phenotypic data obtained using the same antimicrobial susceptibility method and conditions, which allowed us to establish a cut-off for tolerance based on the presence/absence of known genetic determinants.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Large-scale phenotypic and genomic characterization ofListeria monocytogenessusceptibility to quaternary ammonium compounds

Ivanova,

Kragh,

Szarvas

et al. 2023

Preprint

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Listeria monocytogenesis a significant concern for the food industry due to its ability to persist in the food processing environment. Decreased susceptibility to disinfectants is one of the factors that contribute to the persistence ofL. monocytogenes. The objective of this study was to explore the diversity ofL. monocytogenessusceptibility to quaternary ammonium compounds (QACs) using 1,671L. monocytogenesisolates. This was used to determine the phenotype-genotype concordance and characterize genomes of the QAC sensitive and tolerant isolates for stress resistance, virulence and plasmid replicon genes. Distribution of QAC tolerance genes among 37,897 publicly availableL. monocytogenesgenomes were also examined. The minimum inhibitory concentration to QACs was determined by the broth microdilution method and non-sequenced isolates (n=1,244) were whole genome sequenced. Genotype-phenotype concordance was 99% for benzalkonium chloride, DDAC and a commercial QAC based sanitizer. Prevalence of QAC tolerance genes was 23% and 28% in ourL. monocytogenescollection and in the global dataset, respectively.qacHwas the most prevalent gene in our collection (61%), with 19% prevalence in the global dataset. Notably,bcrABCwas most common (72%) globally, while 25% in our collection. Prevalence ofemrCandemrEwas comparable in both datasets, 7% and 2%, respectively. Replicon genes, indicative of plasmid harborage, were detected in 44% of the isolates and associated with the QAC tolerant phenotype. The presented analysis is based on the biggestL. monocytogenescollection in diversity and quantity for characterization of theL. monocytogenesQAC tolerance at both phenotypic and genomic levels.IMPORTANCEContamination ofListeria monocytogeneswithin the food processing environment is of concern to the food industry due to challenges in eradicating the pathogen once it becomes persistent in the environment. Genetic markers associated with increased tolerance to disinfectants have been identified, which alongside factors favor the persistence ofL. monocytogenesin the production environment. By employing a comprehensive large-scale phenotypic testing and genomic analysis our study significantly enhances the understanding of the prevalence of quaternary ammonium compound (QAC) tolerantL. monocytogenesand the genetic determinants associated with the increased tolerance. Furthermore, we report on the prevalence of QAC tolerance genes among 37,897 publicly availableL. monocytogenessequences and their distribution within clonal complexes, isolation sources and geographical locations. As the propagation of QAC tolerance showed not be evenly distributed globally this highlights that understanding the development ofL. monocytogenesdisinfectant tolerance can be monitored using publicly available WGS data.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Large-scale phenotypic and genomic characterization ofListeria monocytogenessusceptibility to quaternary ammonium compounds

Ivanova,

Kragh,

Szarvas

et al. 2023

Preprint

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“…It appears that homeostasis systems do not allow a complete unbalancing of the lipid profile. This is supported by feeding experiments in which different organisms can metabolize a range of fatty acids without damage [ 69 , 70 , 71 , 72 ]. Nevertheless, changes in the composition of the lipid profile can be observed.…”

Section: Discussionmentioning

confidence: 99%

Uptake and Metabolic Conversion of Exogenous Phosphatidylcholines Depending on Their Acyl Chain Structure in Arabidopsis thaliana

Kotlova,

Senik,

Pozhvanov

et al. 2023

IJMS

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Fungi and plants are not only capable of synthesizing the entire spectrum of lipids de novo but also possess a well-developed system that allows them to assimilate exogenous lipids. However, the role of structure in the ability of lipids to be absorbed and metabolized has not yet been characterized in detail. In the present work, targeted lipidomics of phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs), in parallel with morphological phenotyping, allowed for the identification of differences in the effects of PC molecular species introduced into the growth medium, in particular, typical bacterial saturated (14:0/14:0, 16:0/16:0), monounsaturated (16:0/18:1), and typical for fungi and plants polyunsaturated (16:0/18:2, 18:2/18:2) species, on Arabidopsis thaliana. For comparison, the influence of an artificially synthesized (1,2-di-(3-(3-hexylcyclopentyl)-propanoate)-sn-glycero-3-phosphatidylcholine, which is close in structure to archaeal lipids, was studied. The phenotype deviations stimulated by exogenous lipids included changes in the length and morphology of both the roots and leaves of seedlings. According to lipidomics data, the main trends in response to exogenous lipid exposure were an increase in the proportion of endogenic 18:1/18:1 PC and 18:1_18:2 PC molecular species and a decrease in the relative content of species with C18:3, such as 18:3/18:3 PC and/or 16:0_18:3 PC, 16:1_18:3 PE. The obtained data indicate that exogenous lipid molecules affect plant morphology not only due to their physical properties, which are manifested during incorporation into the membrane, but also due to the participation of exogenous lipid molecules in the metabolism of plant cells. The results obtained open the way to the use of PCs of different structures as cellular regulators.

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“…Bacteria were harvested by centrifugation (3000 × g , 5 min), flash-frozen in liquid nitrogen and stored at −80 °C until further processing. Extraction and methylation of fatty acids (FA) were carried out directly on bacterial pellets as described (Touche et al, 2023). Whole-cell FA were first saponified and esterified by methanolic NaOH (1 mL of 3.75 M NaOH in 50% (v/v) methanol for 30 min at 100 °C) followed by methanolic HCl (addition of 2 mL of 3.25 M HCl in 45% (v/v) methanol solution and incubation for 10 min at 80°C).…”

Section: Methodsmentioning

confidence: 99%

Diving into bacterial dormancy: emergence of osmotically stable wall-less forms in an aquatic environment

Carvalho,

Carreaux,

Sartori-Rupp

et al. 2023

Preprint

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Bacteria can respond to environmental stresses by entering a dormant state, called viable but non-culturable (VBNC) state, in which they no longer grow in routine culture media. VBNC pathogens pose thus a significant risk for human and animal health as they are not detected by standard growth-based techniques and can “wake up” back into a vegetative and virulent state. Although hundreds of species were reported to become VBNC in response to different stresses, the molecular mechanisms governing this phenotypic switch remain largely elusive.Here, we characterized the VBNC state transition process in the Gram-positive pathogenListeria monocytogenesin response to nutritional deprivation. By combining fluorescence microscopy, cryo-electron tomography and analytical biochemistry, we found that starvation in mineral water drivesL. monocytogenesinto a VBNC state via a mechanism of cell wall (CW) shedding that generates osmotically stable CW-deficient (CWD) coccoid forms. This phenomenon occurs in multipleL. monocytogenesstrains and in otherListeriaspecies, suggesting it may be a stress-adapting process transversal to theListeriagenus. Transcriptomic and gene-targeted approaches revealed the stress response regulator SigB and the autolysin NamA as major moderators of CW loss and VBNC state transition. Finally, we show that this CWD dormant state is transient as VBNCListeriarevert back to a walled, vegetative and virulent state after passage in embryonated eggs.Our findings provide unprecedented detail on the mechanisms governing the transition to a VBNC state, and reveal that dormant CWD bacterial forms can naturally arise in aquatic environments without osmotic stabilization. This may represent an alternative strategy for bacterial survival in oligotrophic conditions, which can potentially generate public health-threatening reservoirs of undetectable pathogens.

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Growth of Listeria monocytogenes is promoted at low temperature when exogenous unsaturated fatty acids are incorporated in its membrane

Cited by 9 publications

References 39 publications

Large-scale phenotypic and genomic characterization ofListeria monocytogenessusceptibility to quaternary ammonium compounds

Large-scale phenotypic and genomic characterization ofListeria monocytogenessusceptibility to quaternary ammonium compounds

Uptake and Metabolic Conversion of Exogenous Phosphatidylcholines Depending on Their Acyl Chain Structure in Arabidopsis thaliana

Diving into bacterial dormancy: emergence of osmotically stable wall-less forms in an aquatic environment

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