Growth of in vitro Oncidesa plantlets cultured under cold cathode fluorescent lamps with super-elevated CO2 enrichment

Norikane, Atsushi; Silva, Jaime A. Teixeira da; Tanaka, Michio

doi:10.1093/aobpla/plt044

Cited by 9 publications

(3 citation statements)

References 30 publications

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“…''Merry'' (spath), Eucalyptus citriodora, Cymbidium spp. (boat orchid), and Oncidium hybrid (Oncidesa) (Tanaka et al 1992;Nagae et al 1996;Norikane et al 2010Norikane et al , 2013. However, as far as we know, rockwool cubes have not been used as supporting material in TIS.…”

Section: Resultsmentioning

confidence: 99%

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Vidal

Blanco

Cuenca

2015

Plant Cell Tiss Organ Cult

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A protocol for culturing chestnut axillary shoots by temporary immersion in liquid medium was developed. The influence of type of explant, support material, bioreactor, and immersion was investigated for five artificial hybrids and five natural hybrids of Asian and European chestnut selected for resistance to ink disease. The type of explant influenced shoot quality and proliferation rates, and basal explants with callus produced more and longer shoots than apical and nodal segments. Use of rockwool cubes as support material prevented hyperhydricity and allowed proliferation of explants in Murashige and Skoog medium with half-strength nitrates supplemented with 0.22 lM BA and 3 % sucrose, cultured both in plantform TM and RITA Ò vessels with three or six immersions per day and additional aeration of 1 min per hour in the case of plantform TM bioreactors. Basal explants cultured in plantform TM for 5 weeks produced long shoots suitable for rooting, whereas apical and nodal explants cultured in plantform TM or RITA Ò produced shorter shoots that were suitable for maintenance of stock. For most of the clones, similar or higher proliferation rates were observed when cultured in liquid medium than in semisolid medium, with the additional benefit of cost-reduction of the former system. Shoots developed in liquid medium were submitted to ex vitro root induction by dipping in indole-3-butyric acid, and acclimatized under greenhouse conditions. This is the first demonstration of the production of chestnut plantlets from shoots cultured in liquid medium, and the protocol presented here shows good potential for application in large-scale propagation.

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Section: Resultsmentioning

confidence: 99%

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Vidal

Blanco

Cuenca

2015

Plant Cell Tiss Organ Cult

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“…Aside from the auxin-related pathway, GO enrichment and KEGG pathway analyses indicated that the DEGs involved in shoot regeneration were also significantly enriched in photosynthetic and metabolic pathways. In fact, several investigators have reported that the photosynthetic rate markedly influenced shoot regeneration in orchids ( Norikane et al, 2010 , 2013 ). In addition, proteomic and metabolic studies of protocorm from Vanilla planifolia showed that initiation of the shoot regeneration process in Vanilla planifolia revealed a close interrelationship between this species and both photosynthesis and glycolysis ( Palama et al, 2010 ; Tan et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Hormonal Analyses Reveal that YUC-Mediated Auxin Biogenesis Is Involved in Shoot Regeneration from Rhizome in Cymbidium

et al. 2017

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Cymbidium, one of the most important orchid genera in horticulture, can be classified into epiphytic and terrestrial species. Generally, epiphytic Cymbidium seedlings can be easily propagated by tissue culture, but terrestrial seedlings are difficult to propagate. To date, the molecular mechanisms underlying the differences in the ease with which terrestrial and epiphytic cymbidiums can be propagated are largely unknown. Using RNA-sequencing, quantitative reverse transcription PCR and enzyme-linked immunosorbent assay, Cymbidium ‘Xiaofeng’ (CXF), which can be efficiently micropropagated, and terrestrial Cymbidium sinense ‘Qijianbaimo’ (CSQ), which has a low regeneration ability, were used to explore the molecular mechanisms underlying the micropropagation ability of Cymbidium species. To this end, 447 million clean short reads were generated, and 31,264 annotated unigenes were obtained from 10 cDNA libraries. A total of 1,290 differentially expressed genes (DEGs) were identified between CXF and CSQ during shoot induction. Gene ontology (GO) enrichment analysis indicated that the DEGs were significantly enriched in auxin pathway-related GO terms. Further analysis demonstrated that YUC and GH3 family genes, which play crucial roles in the regulation of auxin/IAA (indole-3-acetic acid) metabolism, acted quickly in response to shoot induction culture in vitro and were closely correlated with variation in shoot regeneration between CXF and CSQ. In addition, the study showed that IAA accumulated rapidly and significantly during shoot induction in CXF compared to that in CSQ; in contrast, no significant changes in other hormones were observed between CXF and CSQ. Furthermore, shoot regeneration in CXF was inhibited by a yucasin-auxin biosynthesis inhibitor, indicating that increased IAA level is required for high-frequency shoot regeneration in CXF. In conclusion, our study revealed that YUC-mediated auxin biogenesis is involved in shoot regeneration from rhizome in Cymbidium.

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“…3. Perlite has been used with Limonium latifolium and Solanum tuberosum (Xiao et al 2000), whereas rockwool cubes have been used with Spathiphyllum (Tanaka et al 1992), Eucalyptus citriodora (Nagae et al 1996), Cymbidium (Norikane et al 2010) and Oncidesa (Norikane et al 2013). Vermiculite has been used as a substrate for Eucalyptus camaldulensis (Kirdmanee et al 1995), Garcinia mangostana (Ermayanti et al 1999), Paulownia fortune and Samanea saman explants (Mosaleeyanon et al 2004), and glass beads have been used as a substrate for Rauwolfia serpentina (Goel et al 2007) and Hypericum perforatum (Goel et al 2009).…”

Section: Support Materialsmentioning

confidence: 99%

Micropropagation of axillary shoots of hybrid chestnut (Castanea sativa × C. crenata) in liquid medium in a continuous immersion system

Cuenca

Sánchez

Aldrey

et al. 2017

Plant Cell Tiss Organ Cult

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A protocol for culturing chestnut axillary shoots with the basal sections continuously immersed in liquid medium was developed and the influence of various parameters was assessed: explant type and size, support material, bioreactor size and aeration regime. Shoots excised from eight selected chestnut genotypes were successfully cultured in 10 L bioreactors in liquid Murashige and Skoog medium with half strength nitrates and supplemented with 0.05 mg/L N 6-benzyladenine and 30 g/L sucrose. Forced ventilation and the use of a support material were essential for producing healthy, non-hyperhydric shoots. High proliferation rates were achieved and good quality shoots ready for multiplication, rooting and acclimatization were obtained. Larger leaves showing higher levels of photosynthetic pigments were observed in shoots cultured in bioreactors, suggesting a certain degree of photoautotrophy in shoots cultured under forced ventilation. This is the first demonstration of the production of chestnut shoots in stationary liquid medium, indicating the feasibility of the method for large-scale propagation of the species.

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Growth of in vitro Oncidesa plantlets cultured under cold cathode fluorescent lamps with super-elevated CO2 enrichment

Cited by 9 publications

References 30 publications

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

A temporary immersion system for micropropagation of axillary shoots of hybrid chestnut

Transcriptomic and Hormonal Analyses Reveal that YUC-Mediated Auxin Biogenesis Is Involved in Shoot Regeneration from Rhizome in Cymbidium

Micropropagation of axillary shoots of hybrid chestnut (Castanea sativa × C. crenata) in liquid medium in a continuous immersion system

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