Growth of<i>Cyclotella meneghiniana</i>Kutz. II. Growth and cell composition under different growth rates with different limiting nutrient

Shafik, Hesham M.; Herodek, Sándor; Présing, Mátyås; Vörös, Lajos; Balogh, Katalin

doi:10.1051/limn/1997021

Cited by 18 publications

(15 citation statements)

References 30 publications

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“…The optimum nitrogen and phosphorus mass ratio (N:P ratio) for M. aeruginosa, which was defined as the ratio between the minimum cell quota of nitrate and phosphorus, has been reported as 3.8 (Rhee and Gotham 1980). On the other hand, Shafik et al (1997) indicated that the optimum N:P ratio for Cyclotella meneghiniana was 5.8. From these reports, the N:P ratio of 3.8 and 5.8 were assumed to be an optimum for M. aeruginosa and Cyclotella sp., respectively, and are illustrated in Fig.…”

Section: Resultsmentioning

confidence: 99%

Competition between the cyanobacterium Microcystis aeruginosa and the diatom Cyclotella sp. under nitrogen-limited condition caused by dilution in eutrophic lake

2011

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The improvement of water quality in Lake Tega, Japan, has been carried out by dilution, causing the shift of dominant species from Microcystis aeruginosa to Cyclotella sp. in summer. The disappearance of Microcystis blooms would be related to dilution, but the detail effect has not been understood yet. In this study, the effect of nitrate concentration on the competition between M. aeruginosa and Cyclotella sp. was investigated through the singlespecies and the competitive culture experiments. The single-species culture experiment indicated that the half saturation constants for M. aeruginosa and Cyclotella sp. were 0.016 and 0.234 mg N L −1 , representing that M. aeruginosa would possess a higher affinity to nitrate. On the other hand, the maximum growth rate for Cyclotella sp. was obtained as 0.418 day −1 , which did not represent a significant difference with 0.366 day −1 obtained for M. aeruginosa. The competitive culture experiment revealed that Cyclotella sp. completely dominated over M. aeruginosa at the nitrate concentrations of 0.5 and 2.5 mg N L −1 . The dominance of Cyclotella sp. could be attributed to the difference in the abilities of nitrate storage as well as nitrate uptake. One of the possibilities for the disappearance of Microcystis blooms caused by dilution as observed in Lake Tega could be due to the decrease in nitrate concentration, and the lower N:P ratio seemed not to relate to Microcystis blooms.

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Section: Resultsmentioning

confidence: 99%

Competition between the cyanobacterium Microcystis aeruginosa and the diatom Cyclotella sp. under nitrogen-limited condition caused by dilution in eutrophic lake

2011

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“…Limpens and Berendse (2003) found that the strength of homeostasis may change as a long-term adaptation to site fertility. Furthermore, data from algae suggest that even growth rate itself could be associated with stoichiometric homeostasis (Elrifi and Turpin 1985;Shafik et al 1997;Persson et al 2010), as the relatively constrained biochemical allocations required under fast growth might constrain biomass N:P within a narrow range under situations that permit high growth rates. Similar H values of species within a given functional group and significant differences of H among functional groups suggest that life …”

Section: Discussionmentioning

confidence: 99%

Stoichiometric homeostasis of vascular plants in the Inner Mongolia grassland

et al. 2011

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Stoichiometric homeostasis, the degree to which an organism maintains its C:N:P ratios around a given species- or stage-specific value despite variation in the relative availabilities of elements in its resource supplies, is a key parameter in ecological stoichiometry. However, its regulation and role in affecting organismal and ecosystem processes is still poorly understood in vascular plants. We performed a sand culture experiment and a field nitrogen (N) and phosphorus (P) addition experiment to evaluate the strength of N, P and N:P homeostasis in higher plants in the Inner Mongolia grassland. Our results showed that homeostatic regulation coefficients (H) of vascular plants ranged from 1.93 to 14.5. H varied according to plant species, aboveground and belowground compartments, plant developmental stage, and overall plant nutrient content and N:P ratio. H for belowground and for foliage were inversely related, while H increased with plant developmental stage. H for N (H(N)) was consistently greater than H for P (H(P)) while H for N:P (H(N:P)) was consistently greater than H(N) and H(P). Furthermore, species with greater N and P contents and lower N:P were less homeostatic, suggesting that more homeostatic plants are more conservative nutrient users. The results demonstrate that H of plants encompasses a considerable range but is stronger than that of algae and fungi and weaker than that of animals. This is the first comprehensive evaluation of factors influencing stoichiometric homeostasis in vascular plants.

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“…2 and 3) is reproducible when sufficient agents are used to represent the population . Oliver et al (1981); h Typical value (range), Thomann and Mueller (1987), 20°C, p. 420, l MAX , p. 428; i Tilman and Kilham (1976), l MAX , V net,MAX ; j Shafik et al (1997a), l MAX ; k Shafik et al (1997b), l MAX ; l Sommer (1998), p. 79;m Shuter (1978); n Converted using q C = 5.7-300 fmol C lm -3 (Shafik et al 1997b); o Converted using m = 0.78-4.3 pmol C cell -1 (our data); p Typical, Chapra (1997), p. 614; q Converted using m AVE = 2 ln(2) m 0 (Hellweger and Kianirad 2007) or h:d AVE = ln(2) h:d D ; r Iyengar and Subrahmanyan (1944); s 19 marine and estuarine diatoms, Mizuno (1991); t Cell size split fraction, Synechochoccus elongatus, Mihalcescu et al (2004); u For simulations with biological variability states only. See Table 2.…”

Section: Parameter Valuesmentioning

confidence: 99%

“…See Table 2. Applied to parameters common to Simulations S4, M1 and M2 (l P,MAX , q 0 , l R , k W ); y P-limited culture, Shafik et al (1997a), l D ; z Converted using l D = X Dead /X Alive 9 D (Brussaard et al 1997); A P-limited culture, Shafik et al (1997b), l D ; B Hellweger and Kianirad (2007); C Range of values summarized by Hellweger et al (2003), V net,MAX ; D For simulations with surface area-based uptake only. See Table 2; E A AVE = 250 lm 2 cell -1 , m AVE = 2.6 pmol C cell -1 , based on our data; F Morel (1987); G For simulations with P-dependent uptake only.…”

Section: Parameter Valuesmentioning

confidence: 99%

“…Literature values for C. meneghiniana cell size are generally in the range of 2-20 pmol C cell -1 (Jorgensen 1964; Oliver 1981; converted using 8.6 fmol C Table 2) lm -3 , see ''Methods''), although much larger cells have been observed (290 pmol C cell -1 ; Shafik et al 1997b). The average size of cells in the Charles River is 2.6 pmol C cell -1 , which is on the low side, but within the literature range.…”

Section: Observed Heterogeneity In Cell Sizementioning

confidence: 99%

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Microscale patchiness leads to large and important intraspecific internal nutrient heterogeneity in phytoplankton

et al. 2011

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Phytoplankton stoichiometry or nutrient content has been shown to vary in a number of dimensions (species, condition, time, space), but the heterogeneity within a species at a given time and location, and the underlying mechanisms and importance have not been explored. There are a number of mechanisms that can create intraspecific heterogeneity, and theory suggests it can affect the population growth rate. We studied heterogeneity in P content of the freshwater diatom Cyclotella meneghiniana in the Charles River in Boston. Single-cell observations using synchrotron-based X-ray fluorescence show that the nutrient status varies from P-starved to P-replete. We simulate individual cells using an agent-based model that accounts for a number of mechanisms that can create heterogeneity, including surface area-based uptake, mortality differentiation, stochastic biological variability in states and behavior, macroscale mixing, and microscale nutrient patch encounter. By performing a number of simulations with various mechanisms turned on/off and comparing to data, we conclude that the heterogeneity is mostly due to microscale patchiness (85%). We explore the importance of accounting for heterogeneity in models by performing a simulation with the growth rate based on the population-average internal nutrient, as is done in conventional populationlevel models. This shows that ignoring heterogeneity increases the population growth rate by a factor of 1.47. To account for different heterogeneity in the laboratory and field, population-level ecosystem models should reduce maximum growth rates. The magnitude of this correction depends on local conditions, and in our case, it is a factor of 0.72.

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Growth ofCyclotella meneghinianaKutz. II. Growth and cell composition under different growth rates with different limiting nutrient

Cited by 18 publications

References 30 publications

Competition between the cyanobacterium Microcystis aeruginosa and the diatom Cyclotella sp. under nitrogen-limited condition caused by dilution in eutrophic lake

Competition between the cyanobacterium Microcystis aeruginosa and the diatom Cyclotella sp. under nitrogen-limited condition caused by dilution in eutrophic lake

Stoichiometric homeostasis of vascular plants in the Inner Mongolia grassland

Microscale patchiness leads to large and important intraspecific internal nutrient heterogeneity in phytoplankton

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