Growth of human umbilical-cord blood in longterm haemopoietic cultures

Hows, Jill; Marsh, J. C. W.; Luft, Thomas; Coutinho, L. H.; Testa, Nydia G; Dexter, T. M.; Bradley, B. A.

doi:10.1016/0140-6736(92)90396-k

Cited by 198 publications

(122 citation statements)

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“…tor cells at similar or higher frequency than that of BM. 1,[7][8][9]12 Therefore, UCB, which is normally discarded, has been evaluated as a source of progenitor cells that can be used for the treatment of diseases potentially curable by bone marrow transplantation (BMT). [2][3][4] Major concerns for wider transplant application of UBC have been related to the low total number of progenitor cells obtained by a single collection.…”

Section: Figurementioning

confidence: 99%

“…Therefore, many attempts are ongoing better to understand the biologic and immunologic characteristics of UCB progenitor cells and to evaluate potential for their ex vivo expansion. [7][8][9]11 The structural and functional integrity of the hematopoietic system is maintained by a relatively small population of stem cells that undergo self-renewal. 26,27 Unfortunately, in vitro assays are not available for identifying pluripotential stem cells because of immense heterogeneity within the stem and progenitor cell compartments.…”

Section: Figurementioning

confidence: 99%

“…[3][4][5][6] However, the major concern for future transplant application in adult patients has been related to the low number of progenitor cells that could be obtained by a single UCB collection. Several studies suggested that UCB contains a higher proportion of hematopoietic progenitor cells as compared to adult BM [7][8][9] and therefore the lower number of nucleated cells, present in a single collection, might be compensated by a higher proportion of primitive cells. Since the proliferation of hematopoietic cells is dependent on the presence of hematopoietic cytoCorrespondence: C Almici, Cattedra di Ematologia, Università di Parma, Via Gramsci 14, I-43100 Parma, Italy; Fax: 39 521 292765 Received 17 October 1996; accepted 3 September 1997 kines, 10 the ex vivo expansion potential of UCB has been examined.…”

Section: Introductionmentioning

confidence: 99%

“…Since the proliferation of hematopoietic cells is dependent on the presence of hematopoietic cytoCorrespondence: C Almici, Cattedra di Ematologia, Università di Parma, Via Gramsci 14, I-43100 Parma, Italy; Fax: 39 521 292765 Received 17 October 1996; accepted 3 September 1997 kines, 10 the ex vivo expansion potential of UCB has been examined. 8,9,11 Recently, highly purified CD34 + CD45RA low -CD71 low UCB cells have been reported to possess a much higher ex vivo proliferative potential in comparison to adult BM cells. 12 Moreover, CD34 + CD38 low stem cells purified from BM, UCB and fetal liver have been demonstrated to differ in replicative capacity as determined by the length of their telomeric DNA.…”

Section: Introductionmentioning

confidence: 99%

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Biologic and phenotypic analysis of early hematopoietic progenitor cells in umbilical cord blood

et al. 1997

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Umbilical cord blood (UCB) is an attractive potential alternative to bone marrow (BM) as a source of hematopoietic progenitor cells since the number of progenitors in UCB is similar or even greater than that in normal BM. It was the aim of the present study to analyze the degree of immaturity of UCB progenitor cells. UCB mononuclear (MNC) and/or CD34 + cells were tested for surface antigen phenotype, expression of cytokines receptor, effect of stem cell factor (SCF) on colony growth, resistance to mafosfamide and replating potential. We have found that 34.9 ± 3.4% and 77.9 ± 2.6% of UCB CD34 + cells did not express CD38 and CD45RA antigens, respectively, suggesting that UCB contains a high proportion of immature progenitor cells. By means of three-color analysis, the receptor for SCF was detected on the majority of the CD34 + HLA-DR + subpopulation; in fact, 81.8% ± 4.3% of CD34 + HLA-DR + cells were defined as SCF low and 8.1 ± 1.5% as SCF high . Colony growth of MNC and CD34 + cells was enhanced by the addition of SCF to methylcellulose mixture, resulting in a statistically significant increase in CFU-GM and CFU-GEMM but not in BFU-E numbers. UCB progenitor cells showed a higher resistance to mafosfamide treatment, in comparison to BM; the addition of SCF to the culture medium resulted in a statistically significant increase in mafosfamide concentration required to inhibit 95% of colony growth (P р 0.05). Moreover, as shown by single colony transfer assays, the presence of SCF in primary cultures promoted a significantly higher replating potential for both untreated (42 ± 3.3% vs 21 ± 4.6%, P р 0.018) and mafosfamide-treated samples (62 ± 5.6% vs 44 ± 6.1%, P р 0.018). In conclusion, UCB is a source of progenitor cells with immature characteristics in terms of surface antigen expression, distribution of SCF receptor, resistance to mafosfamide and replating potential. Therefore, UCB progenitor cells represent an ideal candidate population for experimental programs involving gene transfer and ex vivo stem cell expansion.

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Section: Figurementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biologic and phenotypic analysis of early hematopoietic progenitor cells in umbilical cord blood

et al. 1997

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“…It was reported that fresh UCB-derived mononuclear cells (MNCs) seeded on flasks without supportive stromal layers did not form stroma in long-term culture conditions [12]. Wexler et al [13] concluded that neither fresh UCB nor peripheral blood with stem cell mobilization contained MSCs.…”

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confidence: 99%