Growth of human tumor xenografts in nude mice and mice with severe combined immunodeficiency (SCID)

Abstract

Twenty-three fresh tumor specimens obtained at surgery and 5 serially transferable human tumor xenografts were implanted subcutaneously into nude mice and mice with severe combined immunodeficiency (SCID) to compare the take rates of the fresh surgical specimens and the growth rates of the transferable strains. The overall take rates were 65% for the SCID mice and 60% for the nude mice, without any significant difference, although colon carcinoma seemed to have higher acceptance in the SCID mice with a take rate of 6/8. All the serially transferable strains were successfully accepted in the SCID mice, their growth rates being essentially identical to those in the nude mice. These results indicate that the SCID mouse can be used as a human tumor xenograft-mouse system as well as the nude mouse.

Cancer/testis antigen SSX2 enhances invasiveness in MCF-7 cells by repressing ERα signaling

“…However, there was no obvious change in the protein level of the mesenchymal markers such as vimentin (data not shown). MCF-7 cell tumors grow poorly without estradiol treatment in SCID mice (28,29). Our results show that SSX2 favors the growth of MCF-7 xenografts since the growth rate of tumors was roughly four times higher for SSX2 over-expressing cells when compared with the controls (i.e., 81.8 vs. 20%, respectively).…”

BRAK/CXCL14 expression in oral carcinoma cells completely suppresses tumor cell xenografts in SCID mouse

“…Accumulating evidence indicates that cancers grow from cancer stem cells (24) and that tumors progress by interacting with the tumor microenvironment, which is composed of stroma, inflammatory cells and recruited vasculature (3). The nude mouse lacks a thymus, which is essential for the production of T-cells; and SCID mouse is deficient in both B-cells and T-cells, and thus easily accepts tissues from other species (8). In fact, the growth of human tumor xenografts is the same or much greater in the SCID mouse than in nude mouse (11,15).…”

Establishment and characterization of patient-derived tumor xenograft using gastroscopic biopsies in gastric cancer.

“…PDTX models from various tumors have been established, including gastric cancer 22,23,25,26 , which was mostly derived from surgical tumor tissues. In China, the majority of gastric cancer patients are diagnosed as advanced gastric cancer (AGC) and are not candidates for surgery 27 .…”

“…PDTX models from various tumors have been established, such as colorectal cancer 17,18 , breast cancer 15,19 , nonsmall cell lung carcinoma 9,20 , and renal cell carcinoma 21 . PDTX models of gastric cancer using surgical tissues are reported occasionally [22][23][24] ; however, patients with AGC are the most suitable population to evaluate the efficacy of new drugs, and the major method to acquire tumor samples for AGC is gastroscopic biopsy, especially for paired samples before and after chemotherapy. …”