Growth of Gold Nanoparticles in Human Cells

Anshup,; Venkataraman, J. Sai; Subramaniam, Chandramouli; Kumar, Ramesh; Priya, Suma; Kumar, T. Ranjith; Omkumar, Ramakrishnapillai V.; John, and Annie; Pradeep, Thalappil

doi:10.1021/la0519249

Cited by 163 publications

(102 citation statements)

References 21 publications

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“…Biosynthesis of nanoparticles is known to occur in a number of different organisms 28 , and existing reports of longer-term (non light-based) reduction of gold ion solution inside cells 12,13 have been proposed to depend on cellular enzymes or processes. These processes occurring in cells should raise the overall background level of particles, but occur over longer time scales than the photoreduction results presented here, allowing us to confine the reduction to a laser focus region (Figs 2a and 3a).…”

Section: Resultsmentioning

confidence: 99%

“…However, there are several limitations with these methods; bare nanoparticles do not readily penetrate cellular membranes, and even once inside the cell, they cannot move freely. A promising alternative has been to grow gold nanoparticles within bacteria [8][9][10][11] or human cells by infusion of a low-density chloroaurate solution 12,13 , albeit without the means to control location.…”

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confidence: 99%

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Laser-targeted photofabrication of gold nanoparticles inside cells

et al. 2014

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Nanoparticle manipulation is of increasing interest, since they can report single moleculelevel measurements of the cellular environment. Until now, however, intracellular nanoparticle locations have been essentially uncontrollable. Here we show that by infusing a gold ion solution, focused laser light-induced photoreduction allows in situ fabrication of gold nanoparticles at precise locations. The resulting particles are pure gold nanocrystals, distributed throughout the laser focus at sizes ranging from 2 to 20 nm, and remain in place even after removing the gold solution. We demonstrate the spatial control by scanning a laser beam to write characters in gold inside a cell. Plasmonically enhanced molecular signals could be detected from nanoparticles, allowing their use as nano-chemical probes at targeted locations inside the cell, with intracellular molecular feedback. Such light-based control of the intracellular particle generation reaction also offers avenues for in situ plasmonic device creation in organic targets, and may eventually link optical and electron microscopy.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Laser-targeted photofabrication of gold nanoparticles inside cells

et al. 2014

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“…Examples of this include Au NPs grown within cells by incorporating the nanoparticle precursor solution during incubation [329,330], and delivery of Ag NPs by electroporation [331].…”

Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

254

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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“…To visualize nanoparticle (NP) interactions with cells, tissues, and living systems, many optical techniques have been employed, including differential interference contrast (DIC) microscopy 1 and evanescent field-based approaches, such as total internal reflection (TIR) or near-field scanning optical microscopy (NSOM) 2,3 . However, these are high-end analytical approaches, out of reach of most non-specialist labs 4 .…”

Section: Introductionmentioning

confidence: 99%

Identification of Metal Oxide Nanoparticles in Histological Samples by Enhanced Darkfield Microscopy and Hyperspectral Mapping

Roth

Peña

Neu‐Baker

et al. 2015

JoVE

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Nanomaterials are increasingly prevalent throughout industry, manufacturing, and biomedical research. The need for tools and techniques that aid in the identification, localization, and characterization of nanoscale materials in biological samples is on the rise. Currently available methods, such as electron microscopy, tend to be resource-intensive, making their use prohibitive for much of the research community. Enhanced darkfield microscopy complemented with a hyperspectral imaging system may provide a solution to this bottleneck by enabling rapid and less expensive characterization of nanoparticles in histological samples. This method allows for high-contrast nanoscale imaging as well as nanomaterial identification. For this technique, histological tissue samples are prepared as they would be for light-based microscopy. First, positive control samples are analyzed to generate the reference spectra that will enable the detection of a material of interest in the sample. Negative controls without the material of interest are also analyzed in order to improve specificity (reduce false positives). Samples can then be imaged and analyzed using methods and software for hyperspectral microscopy or matched against these reference spectra in order to provide maps of the location of materials of interest in a sample. The technique is particularly well-suited for materials with highly unique reflectance spectra, such as noble metals, but is also applicable to other materials, such as semi-metallic oxides. This technique provides information that is difficult to acquire from histological samples without the use of electron microscopy techniques, which may provide higher sensitivity and resolution, but are vastly more resource-intensive and time-consuming than light microscopy.

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Growth of Gold Nanoparticles in Human Cells

Cited by 163 publications

References 21 publications

Laser-targeted photofabrication of gold nanoparticles inside cells

Laser-targeted photofabrication of gold nanoparticles inside cells

Raman spectroscopy: techniques and applications in the life sciences

Identification of Metal Oxide Nanoparticles in Histological Samples by Enhanced Darkfield Microscopy and Hyperspectral Mapping

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