Growth of Extremely Thermophilic Archaebacteria under Elevated Hyperbaric Conditions<sup>a</sup>

Sturm, F. J.; Parameswaran, A. K.; Provan, C. N.; Kelly, Robert M.

doi:10.1111/j.1749-6632.1987.tb23809.x

Cited by 4 publications

(1 citation statement)

References 6 publications

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“…Though the addition of yeast extract to growth media does not influence growth significantly (except at the higher growth temperatures, i.e., 105°C [49]), it does have a profound effect on the amount of sulfide produced during growth [48,49]. occulturn is a strict chemolithotroph, dependent on COz as its carbon source.…”

Section: Bacteria From Submarine Geothermal Sitesmentioning

confidence: 99%

Extremely Thermophilic Archaebacteria: Biological and Engineering Considerations

Kelly

Deming

1988

Biotechnology Progress

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~ Interdisciplinary interactions between molecular biologists, microbial ecologists, and biochemical engineers portend weU for the biotechnological exploitation of novel, extremely thermophdic methanogens and sulfur-metabolizing archaebacteriu.[ntroduction The field of biotechnology presently relies heavily on the use of genetic engineering to make the production of a variety of biological products feasme in both a technical ind economic sense. However, there is growing interest in determining if "wild-type'' microorganisms that function under extreme conditions (i.e., high temperature, high pressure, low pH, etc.) or their products can be put directly to effective biotechnological use. The exploitation of these novel microorganisms need not preclude the use of recombinant DNA technology; on the contrary, the more we learn about the "natural" functions of bacteria from extreme environments, the greater the possibilities for genetic engineering. Among the extreme environments in which microorganisms have been found to prosper, few have generated as much scientific interest in recent years as those characterized by high temperatures [ I ] . Following reports of evidence that bacterial growth and activity may occur under deep-sea pressures at temperatures to at least 250°C [ 2 4 ] (well in excess of conventional limits) efforts by several groups of researchers [S7] to acquire more samples from submarine hydrothermal vents, hot springs, and other areas of geothermal activity in search of novel extremely thermophilic microorganisms have intensified. Though these efforts have not yet yielded confirmation of the original report [3], not quelled the controversy (pro [8] and con [9-111) that it engendered, there has been notable progress toward the isolation of new thermophiles that #Correspondence should be address to Robert M . Kelly Yody W. Deming is presently at the School of Oceanography llniversity of Washington, WB-10, Seattle, WA 98195. grow at higher and higher temperatures. In particular, Stetter and colleagues [5, 6, 12, 131 have reported and

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Section: Bacteria From Submarine Geothermal Sitesmentioning

confidence: 99%

Extremely Thermophilic Archaebacteria: Biological and Engineering Considerations

Kelly

Deming

1988

Biotechnology Progress

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Effect of hyperbaric oxygen and carbon dioxide on heterotrophic growth of the extreme thermophile Sulfolobus acidocaldarius

Kelly

1988

Biotech & Bioengineering

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Recent interest in extremely thermophilic archaebacteria comes, in part, from a variety of potential biotechnological applications that have been proposed. These range from highly thermostable enzymes to possible novel biotransformations. Suljolobus acidocaldarius, for example, one of the most studied of the extreme thermophiles, has already been identified as playing a role in coal desulfurization and ore leaching processes. '3, Relatively little has been done thus far, however, to examine the engineering considerations associated with the growth of extremely thermophilic bacteria. Since utilization of several nutrients present as sparingly soluble dissolved gases is often characteristic of these microorganisms, gas-liquid mass transfer and sensitivity to dissolved gas tensions are important factors to be examined.It has been suggested that the use of elevated partial pressures of nutrient gases might provide a means to overcome solubility limitations on mass transfer at the temperatures at which extreme thermophiles g r o~.~,~ It is well known that, for the growth of aerobic microorganisms, the transfer of oxygen from the gas phase to the liquid phase is often rate limiting, especially when biomass concentrations and oxygen demand are high. For aerobic extreme thermophiles, although the mass transfer coefficient in a given system would be expected to increase at high temp e r a t~r e ,~ mass transfer rates may not increase because of the decrease in oxygen solubility. It is not clear the degree to which oxygen (or other gas) mass transfer limitations exist for microorganisms growing at elevated temperatures. Also, there is almost no information available that quantifies the sensitivity of extreme thermophiles to dissolved gas tension^.^'^ In this study, the aerobic, extremely thermophilic archaebacterium, Suljolobus acidocaldarius, was cultured heterotrophically in an autoclave bioreactor under elevated partial pressures of CO, and air with hyperbaric helium used to obtain the desired incubation pressure. Of interest was the effect of elevated nutrient gas partial pressures on growth

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Sulfur gas tolerance and toxicity of co-utilizing and methanogenic bacteria

Vega

Klasson

Kimmel

et al. 1990

Appl Biochem Biotechnol

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Growth of Extremely Thermophilic Archaebacteria under Elevated Hyperbaric Conditions^a

Cited by 4 publications

References 6 publications

Extremely Thermophilic Archaebacteria: Biological and Engineering Considerations

Extremely Thermophilic Archaebacteria: Biological and Engineering Considerations

Effect of hyperbaric oxygen and carbon dioxide on heterotrophic growth of the extreme thermophile Sulfolobus acidocaldarius

Sulfur gas tolerance and toxicity of co-utilizing and methanogenic bacteria

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Growth of Extremely Thermophilic Archaebacteria under Elevated Hyperbaric Conditionsa

Cited by 4 publications

References 6 publications

Extremely Thermophilic Archaebacteria: Biological and Engineering Considerations

Extremely Thermophilic Archaebacteria: Biological and Engineering Considerations

Effect of hyperbaric oxygen and carbon dioxide on heterotrophic growth of the extreme thermophile Sulfolobus acidocaldarius

Sulfur gas tolerance and toxicity of co-utilizing and methanogenic bacteria

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Growth of Extremely Thermophilic Archaebacteria under Elevated Hyperbaric Conditions^a